Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   pm9n_RS00435 Genome accession   NZ_CP028927
Coordinates   110436..110870 (+) Length   144 a.a.
NCBI ID   WP_005741096.1    Uniprot ID   -
Organism   Pasteurella multocida strain 9N     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 105436..115870
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  pm9n_RS00405 (pm9n_00405) tadA 105547..106047 (-) 501 WP_005755875.1 tRNA adenosine(34) deaminase TadA -
  pm9n_RS00410 (pm9n_00410) - 106062..106913 (-) 852 WP_010906470.1 thymidylate synthase -
  pm9n_RS00415 (pm9n_00415) lgt 106910..107722 (-) 813 WP_010906471.1 prolipoprotein diacylglyceryl transferase -
  pm9n_RS00420 (pm9n_00420) - 107732..108526 (-) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  pm9n_RS00425 (pm9n_00425) rppH 108529..109125 (-) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  pm9n_RS00430 (pm9n_00430) ampD 109710..110276 (-) 567 WP_005751074.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  pm9n_RS00435 (pm9n_00435) pilA 110436..110870 (+) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  pm9n_RS00440 (pm9n_00440) pilB 110870..112255 (+) 1386 WP_010906472.1 GspE/PulE family protein Machinery gene
  pm9n_RS00445 (pm9n_00445) - 112255..113466 (+) 1212 WP_005722560.1 type II secretion system F family protein -
  pm9n_RS00450 (pm9n_00450) - 113466..114188 (+) 723 WP_010906474.1 prepilin peptidase -
  pm9n_RS00455 (pm9n_00455) coaE 114203..114823 (+) 621 WP_010906475.1 dephospho-CoA kinase -
  pm9n_RS00460 (pm9n_00460) yacG 114834..115037 (+) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  pm9n_RS00465 (pm9n_00465) - 115037..115306 (+) 270 WP_005722568.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15058.48 Da        Isoelectric Point: 9.1281

>NTDB_id=288623 pm9n_RS00435 WP_005741096.1 110436..110870(+) (pilA) [Pasteurella multocida strain 9N]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=288623 pm9n_RS00435 WP_005741096.1 110436..110870(+) (pilA) [Pasteurella multocida strain 9N]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCTGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACAGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG
CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTTAATTGTGGTGCTC
AATCTGACATTTTCCCAGCAGGATTTTGCGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.194

100

0.632
  pilA Haemophilus influenzae Rd KW20

61.111

100

0.611
  pilA Glaesserella parasuis strain SC1401

52.349

100

0.542
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.806

100

0.368
  pilA Vibrio cholerae strain A1552

36.806

100

0.368
  pilA Vibrio cholerae C6706

36.806

100

0.368

Multiple sequence alignment