Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   Sa5Y_RS01380 Genome accession   NZ_CP028892
Coordinates   280478..281494 (+) Length   338 a.a.
NCBI ID   WP_094392825.1    Uniprot ID   -
Organism   Vibrio cholerae strain Sa5Y     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 275478..286494
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Sa5Y_RS01365 (Sa5Y_VC00269) - 276019..276750 (-) 732 WP_000495226.1 glutathione peroxidase -
  Sa5Y_RS01370 (Sa5Y_VC00270) oxyR 276890..277783 (+) 894 WP_094392824.1 DNA-binding transcriptional regulator OxyR -
  Sa5Y_RS01375 (Sa5Y_VC00271) - 277858..280335 (-) 2478 WP_000668742.1 penicillin-binding protein 1A -
  Sa5Y_RS01380 (Sa5Y_VC00272) pilM 280478..281494 (+) 1017 WP_094392825.1 type IV pilus assembly protein PilM Machinery gene
  Sa5Y_RS01385 (Sa5Y_VC00273) pilN 281484..282068 (+) 585 WP_000902746.1 PilN domain-containing protein Machinery gene
  Sa5Y_RS01390 (Sa5Y_VC00274) pilO 282061..282651 (+) 591 WP_162298793.1 type 4a pilus biogenesis protein PilO Machinery gene
  Sa5Y_RS01395 (Sa5Y_VC00275) pilP 282641..283159 (+) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  Sa5Y_RS01400 (Sa5Y_VC00276) pilQ 283185..284900 (+) 1716 WP_162298794.1 type IV pilus secretin PilQ family protein Machinery gene
  Sa5Y_RS01405 (Sa5Y_VC00277) aroK 285087..285611 (+) 525 WP_000818616.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37734.46 Da        Isoelectric Point: 7.3328

>NTDB_id=288201 Sa5Y_RS01380 WP_094392825.1 280478..281494(+) (pilM) [Vibrio cholerae strain Sa5Y]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELNLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=288201 Sa5Y_RS01380 WP_094392825.1 280478..281494(+) (pilM) [Vibrio cholerae strain Sa5Y]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGTGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAACCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCT
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACACAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
TTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCACTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGTAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-221)

Type IV pilus assembly protein PilM; (PilM_2)

(241-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

99.704

100

0.997
  pilM Vibrio campbellii strain DS40M4

48.225

100

0.482

Multiple sequence alignment