Detailed information NTG0285085

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: DA379_RS10120
• Genome accession: NZ_CP028441
• Coordinates: 2085518..2086132 (-)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain 131-4
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2080518..2091132

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DA379_RS10095	rpsT	2080818..2081084 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
DA379_RS10100	holA	2081101..2082142 (-)	1042	Protein_1975	DNA polymerase III subunit delta	-
DA379_RS19535	-	2082182..2082336 (-)	155	Protein_1976	hypothetical protein	-
DA379_RS10105	-	2082377..2082511 (+)	135	WP_003152870.1	YqzM family protein	-
DA379_RS10110	comEC	2082530..2084881 (-)	2352	WP_003152869.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
DA379_RS10115	-	2084882..2085451 (-)	570	WP_003152868.1	ComE operon protein 2	-
DA379_RS10120	comEA	2085518..2086132 (-)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
DA379_RS10125	comER	2086191..2087012 (+)	822	WP_003152866.1	late competence protein ComER	-
DA379_RS10130	-	2087081..2087818 (-)	738	WP_003152865.1	class I SAM-dependent methyltransferase	-
DA379_RS10135	rsfS	2087815..2088171 (-)	357	WP_003152864.1	ribosome silencing factor	-
DA379_RS10140	yqeK	2088168..2088749 (-)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
DA379_RS10145	-	2088739..2089308 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
DA379_RS10150	yhbY	2089319..2089609 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
DA379_RS10155	aroE	2089603..2090445 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=285085 DA379_RS10120 WP_003152867.1 2085518..2086132(-) (comEA) [Bacillus velezensis strain 131-4]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=285085 DA379_RS10120 WP_003152867.1 2085518..2086132(-) (comEA) [Bacillus velezensis strain 131-4]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363