Detailed information NTG0285033

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: DA378_RS18750
• Genome accession: NZ_CP028440
• Coordinates: 3759398..3760012 (+)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain J7-1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3754398..3765012

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DA378_RS18715	aroE	3755085..3755927 (+)	843	WP_003152856.1	shikimate dehydrogenase	-
DA378_RS18720	yhbY	3755921..3756211 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
DA378_RS18725	-	3756222..3756791 (+)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
DA378_RS18730	yqeK	3756781..3757362 (+)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
DA378_RS18735	rsfS	3757359..3757715 (+)	357	WP_003152864.1	ribosome silencing factor	-
DA378_RS18740	-	3757712..3758449 (+)	738	WP_003152865.1	class I SAM-dependent methyltransferase	-
DA378_RS18745	comER	3758518..3759339 (-)	822	WP_003152866.1	late competence protein ComER	-
DA378_RS18750	comEA	3759398..3760012 (+)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
DA378_RS18755	-	3760079..3760648 (+)	570	WP_003152868.1	ComE operon protein 2	-
DA378_RS18760	comEC	3760649..3763000 (+)	2352	WP_003152869.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
DA378_RS18765	-	3763019..3763153 (-)	135	WP_003152870.1	YqzM family protein	-
DA378_RS19565	-	3763194..3763348 (+)	155	Protein_3610	hypothetical protein	-
DA378_RS18770	holA	3763388..3764429 (+)	1042	Protein_3611	DNA polymerase III subunit delta	-
DA378_RS18775	rpsT	3764446..3764712 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=285033 DA378_RS18750 WP_003152867.1 3759398..3760012(+) (comEA) [Bacillus velezensis strain J7-1]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=285033 DA378_RS18750 WP_003152867.1 3759398..3760012(+) (comEA) [Bacillus velezensis strain J7-1]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363