Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   DA377_RS18790 Genome accession   NZ_CP028439
Coordinates   3751577..3752014 (+) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain 8-2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3746577..3757014
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DA377_RS18775 - 3749435..3749797 (-) 363 WP_003156585.1 helix-turn-helix domain-containing protein -
  DA377_RS18780 hxlA 3750029..3750664 (+) 636 WP_003156586.1 3-hexulose-6-phosphate synthase -
  DA377_RS18785 hxlB 3750661..3751218 (+) 558 WP_003156587.1 6-phospho-3-hexuloisomerase -
  DA377_RS18790 nucA/comI 3751577..3752014 (+) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  DA377_RS18795 nin/comJ 3752036..3752434 (+) 399 WP_003156589.1 competence protein ComJ Regulator
  DA377_RS18800 - 3752475..3752693 (-) 219 Protein_3624 family 1 glycosylhydrolase -
  DA377_RS20010 - 3752692..3752970 (-) 279 WP_107890409.1 family 1 glycosylhydrolase -
  DA377_RS18810 - 3752912..3753232 (-) 321 WP_003156590.1 YckD family protein -
  DA377_RS18815 - 3753304..3753738 (-) 435 WP_003156592.1 RDD family protein -
  DA377_RS18820 - 3753753..3754889 (-) 1137 WP_003156594.1 zinc-dependent alcohol dehydrogenase -
  DA377_RS18825 - 3754954..3756156 (-) 1203 WP_003156595.1 GTP-binding protein -
  DA377_RS19880 - 3756295..3756431 (+) 137 Protein_3630 molybdopterin dinucleotide binding domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=284966 DA377_RS18790 WP_003156588.1 3751577..3752014(+) (nucA/comI) [Bacillus velezensis strain 8-2]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=284966 DA377_RS18790 WP_003156588.1 3751577..3752014(+) (nucA/comI) [Bacillus velezensis strain 8-2]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment