Detailed information NTG0284924

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: DA377_RS08220
• Genome accession: NZ_CP028439
• Coordinates: 1555037..1555651 (+)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain 8-2
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1550037..1560651

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DA377_RS08185	aroE	1550724..1551566 (+)	843	WP_003152856.1	shikimate dehydrogenase	-
DA377_RS08190	yhbY	1551560..1551850 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
DA377_RS08195	-	1551861..1552430 (+)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
DA377_RS08200	yqeK	1552420..1553001 (+)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
DA377_RS08205	rsfS	1552998..1553354 (+)	357	WP_003152864.1	ribosome silencing factor	-
DA377_RS08210	-	1553351..1554088 (+)	738	WP_003152865.1	class I SAM-dependent methyltransferase	-
DA377_RS08215	comER	1554157..1554978 (-)	822	WP_003152866.1	late competence protein ComER	-
DA377_RS08220	comEA	1555037..1555651 (+)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
DA377_RS08225	-	1555718..1556287 (+)	570	WP_003152868.1	ComE operon protein 2	-
DA377_RS08230	comEC	1556288..1558639 (+)	2352	WP_003152869.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
DA377_RS08235	-	1558658..1558792 (-)	135	WP_003152870.1	YqzM family protein	-
DA377_RS19530	-	1558833..1558987 (+)	155	Protein_1555	hypothetical protein	-
DA377_RS08240	holA	1559027..1560068 (+)	1042	Protein_1556	DNA polymerase III subunit delta	-
DA377_RS08245	rpsT	1560085..1560351 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=284924 DA377_RS08220 WP_003152867.1 1555037..1555651(+) (comEA) [Bacillus velezensis strain 8-2]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=284924 DA377_RS08220 WP_003152867.1 1555037..1555651(+) (comEA) [Bacillus velezensis strain 8-2]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363