Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   EGV94_RS26195 Genome accession   NZ_CP033685
Coordinates   5482647..5483186 (-) Length   179 a.a.
NCBI ID   WP_003141352.1    Uniprot ID   A0A0H2ZFW8
Organism   Pseudomonas aeruginosa strain H26023     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5477647..5488186
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGV94_RS26175 (EGV94_26120) - 5478333..5480588 (-) 2256 WP_003134886.1 DUF1631 domain-containing protein -
  EGV94_RS26180 (EGV94_26125) nadC 5480874..5481722 (+) 849 WP_003141348.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EGV94_RS26190 (EGV94_26135) - 5481885..5482583 (-) 699 WP_016254081.1 hypothetical protein -
  EGV94_RS26195 (EGV94_26140) pilE 5482647..5483186 (-) 540 WP_003141352.1 pilin Machinery gene
  EGV94_RS26200 (EGV94_26145) pilB 5483415..5485118 (+) 1704 WP_003141354.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EGV94_RS26205 (EGV94_26150) pilC 5485122..5486339 (+) 1218 WP_016254080.1 type II secretion system F family protein Machinery gene
  EGV94_RS26210 (EGV94_26155) pilD 5486342..5487214 (+) 873 WP_003141356.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  EGV94_RS26215 (EGV94_26160) coaE 5487211..5487822 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  EGV94_RS26220 (EGV94_26165) yacG 5487819..5488019 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 18083.45 Da        Isoelectric Point: 5.0804

>NTDB_id=284260 EGV94_RS26195 WP_003141352.1 5482647..5483186(-) (pilE) [Pseudomonas aeruginosa strain H26023]
MKAQKGFTLIELMIVVAIIGILAAVALPAYQDYTIRARVTEGVGLAASAKTLIGDSSATAGELAASARVWNAQAGNAGAT
SKYVTSVRIAEATGEITVTFNATNVGNIPADSTLVFTPYVQNAAGAPTQLGASYAAGVTGSIDWGCASESNAVSSGPDRN
MPALTAGTLPARFAPSECR

Nucleotide


Download         Length: 540 bp        

>NTDB_id=284260 EGV94_RS26195 WP_003141352.1 5482647..5483186(-) (pilE) [Pseudomonas aeruginosa strain H26023]
ATGAAAGCTCAGAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATTCTTGCTGCCGTCGCTTT
GCCGGCATATCAGGATTACACCATTCGTGCTCGCGTGACAGAGGGGGTTGGTCTGGCTGCCAGCGCCAAGACGCTTATTG
GCGATAGCTCTGCCACTGCCGGTGAGCTAGCCGCTTCGGCAAGGGTCTGGAATGCTCAAGCCGGTAACGCCGGTGCTACC
AGTAAGTATGTGACCTCTGTGCGAATTGCAGAGGCGACTGGTGAAATCACTGTTACTTTCAATGCCACAAACGTGGGTAA
TATTCCGGCTGACTCTACCCTGGTATTTACTCCCTATGTGCAGAATGCTGCCGGTGCCCCGACTCAATTGGGTGCCAGTT
ATGCTGCCGGTGTGACTGGCTCTATTGACTGGGGTTGTGCATCGGAATCCAATGCGGTTTCCAGTGGTCCCGACCGCAAT
ATGCCTGCCCTGACTGCAGGTACCCTGCCGGCTCGTTTCGCTCCGAGCGAATGCCGCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(36-179)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZFW8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

42.64

100

0.469
  pilA Ralstonia pseudosolanacearum GMI1000

43.243

100

0.447
  pilA2 Legionella pneumophila str. Paris

41.808

98.883

0.413
  pilA2 Legionella pneumophila strain ERS1305867

41.243

98.883

0.408
  comP Acinetobacter baylyi ADP1

38.333

100

0.385
  pilE Neisseria gonorrhoeae strain FA1090

35.714

100

0.363