Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   C9I28_RS07405 Genome accession   NZ_CP028324
Coordinates   1662632..1664320 (-) Length   562 a.a.
NCBI ID   WP_229415941.1    Uniprot ID   -
Organism   Pseudoduganella armeniaca strain ZMN-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1657632..1669320
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C9I28_RS07390 (C9I28_07390) - 1658159..1659997 (+) 1839 WP_107140922.1 alpha-amylase family glycosyl hydrolase -
  C9I28_RS28530 - 1660046..1660297 (+) 252 WP_229415939.1 hypothetical protein -
  C9I28_RS07395 (C9I28_07395) - 1660195..1661400 (+) 1206 WP_229415940.1 MFS transporter -
  C9I28_RS07400 (C9I28_07400) pilC 1661407..1662621 (-) 1215 WP_107140923.1 type II secretion system F family protein Machinery gene
  C9I28_RS07405 (C9I28_07405) pilF 1662632..1664320 (-) 1689 WP_229415941.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C9I28_RS07410 (C9I28_07410) - 1664452..1665723 (-) 1272 WP_107140925.1 HlyC/CorC family transporter -
  C9I28_RS07415 (C9I28_07415) - 1665910..1667136 (+) 1227 WP_107140926.1 glutamate carboxypeptidase -
  C9I28_RS07420 (C9I28_07420) uraH 1667121..1667474 (-) 354 WP_107140927.1 hydroxyisourate hydrolase -
  C9I28_RS07425 (C9I28_07425) - 1667565..1669091 (+) 1527 WP_229415942.1 carboxylesterase/lipase family protein -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 61035.67 Da        Isoelectric Point: 6.2548

>NTDB_id=284112 C9I28_RS07405 WP_229415941.1 1662632..1664320(-) (pilF) [Pseudoduganella armeniaca strain ZMN-3]
MSGLARALMQAGRLTPPQADALQKKSHADKLPFIDVLLASGAVNSRDLALFCAETFAYPMLDLHAFAVAALPQKLIDPKL
MQSQRVVALAKRGNKMSVAISDPTNTQALDQIKFQTESSVEPVIVPHDALVRLLQELGKSSEQLMGELAGDEGEIQFAEE
QESTTVAEAPATDVEDAPIVRFLNKMLMDAVNMGASDLHFEPFEKFYRIRFRVDGVLIEHAQPPIAIKDKLVSRIKVLAR
LDISEKRVPQDGRMRLIVSPTKTIDLRISTLPTLFGEKTVMRILDATQAQMGIDALGYDPDQKALLLDAIQRPYGMVLVT
GPTGSGKTVSLYTCLNILNKPGINISTAEDPAEINLPGVNQVNVNDKAGLTFPVALKSFLRQDPDIIMVGEIRDLETADI
AIKAAQTGHMVFSTLHTNDAPSTLTRLMNMGVAPFNIASSIVLITAQRLARRLCTCKQPVDISNDLLLRAGFKQHDLDGS
WKPYGPVGCERCNGTGYKGRVGIYQIMPITPAIEALILAHGNAMQIAAQSESEGVKSLRQSGLVKVKAGLTSLEEVLGCT
NE

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=284112 C9I28_RS07405 WP_229415941.1 1662632..1664320(-) (pilF) [Pseudoduganella armeniaca strain ZMN-3]
ATGTCGGGCCTGGCTCGTGCCCTGATGCAGGCGGGGCGGCTCACGCCCCCGCAGGCGGACGCACTGCAAAAAAAATCCCA
CGCCGACAAGCTGCCCTTCATCGACGTGCTGCTGGCCAGCGGCGCCGTCAATTCACGCGACTTGGCGCTGTTCTGCGCCG
AGACGTTCGCCTACCCGATGCTGGACCTGCACGCGTTTGCCGTCGCCGCGCTGCCCCAGAAGCTGATCGACCCGAAGCTG
ATGCAGAGCCAGCGCGTGGTGGCGCTGGCCAAGCGCGGCAACAAGATGTCGGTGGCGATTTCCGACCCCACCAACACGCA
GGCGCTGGACCAGATCAAGTTCCAGACCGAGTCGTCGGTGGAACCGGTGATCGTGCCGCACGACGCGCTGGTGCGCTTGC
TGCAGGAACTGGGCAAGAGCAGCGAGCAGCTGATGGGCGAGCTGGCCGGCGACGAGGGCGAGATCCAGTTCGCCGAGGAA
CAGGAATCGACGACCGTGGCGGAAGCGCCGGCGACCGACGTCGAGGACGCGCCGATCGTGCGCTTCCTGAACAAGATGCT
GATGGACGCCGTCAACATGGGCGCCTCCGACCTGCACTTCGAGCCGTTCGAGAAGTTCTACCGCATCCGCTTCCGTGTCG
ACGGCGTGCTGATCGAACACGCCCAGCCCCCGATCGCCATCAAGGACAAGCTGGTCTCGCGCATCAAGGTGCTGGCGCGG
CTGGACATCTCGGAAAAGCGCGTGCCGCAGGATGGCCGCATGCGCCTGATCGTCTCGCCGACCAAAACCATCGACCTGCG
TATCTCGACGCTGCCGACGCTGTTCGGCGAGAAGACCGTGATGCGTATCCTGGACGCGACCCAGGCCCAGATGGGCATCG
ACGCGCTGGGCTACGATCCGGACCAGAAGGCGTTGCTGCTGGACGCGATCCAGCGTCCGTACGGCATGGTGCTGGTGACG
GGGCCGACGGGTTCCGGCAAGACCGTCTCCCTGTACACCTGCCTGAACATCCTGAACAAGCCGGGCATCAATATCTCGAC
GGCGGAAGACCCGGCCGAGATCAACCTGCCCGGCGTGAACCAGGTCAACGTCAACGACAAGGCGGGGCTGACGTTCCCGG
TCGCGCTGAAGTCCTTCCTGCGCCAGGACCCGGACATCATCATGGTCGGCGAGATCCGCGACCTGGAGACGGCGGACATC
GCCATCAAGGCGGCGCAGACGGGCCACATGGTGTTCTCCACCTTGCACACCAACGACGCGCCATCGACCTTGACGCGCCT
GATGAACATGGGCGTGGCGCCGTTCAACATCGCCTCGTCGATCGTGCTGATCACGGCGCAGCGCCTGGCGCGCCGGCTGT
GCACCTGCAAGCAGCCGGTCGATATCTCCAACGACCTGCTGCTGCGGGCCGGGTTCAAGCAGCACGACCTGGACGGCAGC
TGGAAGCCGTACGGCCCGGTGGGCTGCGAGCGCTGCAACGGCACCGGCTACAAGGGCCGGGTGGGCATCTACCAGATCAT
GCCGATCACGCCGGCCATCGAGGCGCTGATCCTGGCGCACGGTAACGCGATGCAGATCGCGGCGCAGTCGGAAAGCGAAG
GCGTGAAGTCCTTGCGCCAGTCCGGCCTCGTCAAGGTCAAGGCAGGCCTGACCAGCCTGGAAGAAGTGCTGGGCTGCACC
AACGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-142)

Type II/IV secretion system protein (T2SSE)

(185-452)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

53.464

100

0.536
  pilB Acinetobacter baylyi ADP1

52.837

100

0.53
  pilB Acinetobacter baumannii D1279779

55.871

93.95

0.525
  pilB Legionella pneumophila strain ERS1305867

48.577

100

0.486
  pilB Vibrio cholerae strain A1552

46.099

100

0.463
  pilB Vibrio parahaemolyticus RIMD 2210633

44.64

100

0.452
  pilB Vibrio campbellii strain DS40M4

44.876

100

0.452
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.192

100

0.397
  pilF Thermus thermophilus HB27

37.887

97.687

0.37

Multiple sequence alignment