Detailed information NTG0283030

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: C7M25_RS11685
• Genome accession: NZ_CP028211
• Coordinates: 2301624..2302238 (+)
• Length: 204 a.a.
• NCBI ID: WP_043021637.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain SRCM102747
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2296624..2307238

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C7M25_RS11650 (C7M25_02340)	aroE	2297311..2298153 (+)	843	WP_094031849.1	shikimate dehydrogenase	-
C7M25_RS11655 (C7M25_02341)	yhbY	2298147..2298437 (+)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
C7M25_RS11660 (C7M25_02342)	-	2298448..2299017 (+)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
C7M25_RS11665 (C7M25_02343)	yqeK	2299007..2299567 (+)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
C7M25_RS11670 (C7M25_02344)	rsfS	2299585..2299941 (+)	357	WP_007408260.1	ribosome silencing factor	-
C7M25_RS11675 (C7M25_02345)	-	2299938..2300675 (+)	738	WP_076424998.1	class I SAM-dependent DNA methyltransferase	-
C7M25_RS11680 (C7M25_02346)	comER	2300744..2301565 (-)	822	WP_012118027.1	late competence protein ComER	-
C7M25_RS11685 (C7M25_02347)	comEA	2301624..2302238 (+)	615	WP_043021637.1	helix-hairpin-helix domain-containing protein	Machinery gene
C7M25_RS11690 (C7M25_02348)	-	2302305..2302874 (+)	570	WP_094031848.1	ComE operon protein 2	-
C7M25_RS11695 (C7M25_02349)	comEC	2302875..2305226 (+)	2352	WP_094031847.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
C7M25_RS11700	-	2305245..2305379 (-)	135	WP_003152870.1	YqzM family protein	-
C7M25_RS11705	-	2305420..2305571 (+)	152	Protein_2254	hypothetical protein	-
C7M25_RS11710 (C7M25_02350)	holA	2305611..2306652 (+)	1042	Protein_2255	DNA polymerase III subunit delta	-
C7M25_RS11715 (C7M25_02351)	rpsT	2306669..2306935 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22165.31 Da
• Isoelectric Point: 6.4888

>NTDB_id=283030 C7M25_RS11685 WP_043021637.1 2301624..2302238(+) (comEA) [Bacillus velezensis strain SRCM102747]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=283030 C7M25_RS11685 WP_043021637.1 2301624..2302238(+) (comEA) [Bacillus velezensis strain SRCM102747]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363