Detailed information NTG0282969

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: C7M24_RS12940
• Genome accession: NZ_CP028210
• Coordinates: 2685897..2686511 (-)
• Length: 204 a.a.
• NCBI ID: WP_043021637.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain SRCM102746
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2680897..2691511

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C7M24_RS12910 (C7M24_02598)	rpsT	2681200..2681466 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
C7M24_RS12915 (C7M24_02599)	holA	2681483..2682524 (-)	1042	Protein_2523	DNA polymerase III subunit delta	-
C7M24_RS12920	-	2682564..2682715 (-)	152	Protein_2524	hypothetical protein	-
C7M24_RS12925	-	2682756..2682890 (+)	135	WP_003152870.1	YqzM family protein	-
C7M24_RS12930 (C7M24_02600)	comEC	2682909..2685260 (-)	2352	WP_094031847.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
C7M24_RS12935 (C7M24_02601)	-	2685261..2685830 (-)	570	WP_094031848.1	ComE operon protein 2	-
C7M24_RS12940 (C7M24_02602)	comEA	2685897..2686511 (-)	615	WP_043021637.1	helix-hairpin-helix domain-containing protein	Machinery gene
C7M24_RS12945 (C7M24_02603)	comER	2686570..2687391 (+)	822	WP_012118027.1	late competence protein ComER	-
C7M24_RS12950 (C7M24_02604)	-	2687460..2688197 (-)	738	WP_076424998.1	class I SAM-dependent DNA methyltransferase	-
C7M24_RS12955 (C7M24_02605)	rsfS	2688194..2688550 (-)	357	WP_007408260.1	ribosome silencing factor	-
C7M24_RS12960 (C7M24_02606)	yqeK	2688568..2689128 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
C7M24_RS12965 (C7M24_02607)	-	2689118..2689687 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
C7M24_RS12970 (C7M24_02608)	yhbY	2689698..2689988 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
C7M24_RS12975 (C7M24_02609)	aroE	2689982..2690824 (-)	843	WP_094031849.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22165.31 Da
• Isoelectric Point: 6.4888

>NTDB_id=282969 C7M24_RS12940 WP_043021637.1 2685897..2686511(-) (comEA) [Bacillus velezensis strain SRCM102746]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=282969 C7M24_RS12940 WP_043021637.1 2685897..2686511(-) (comEA) [Bacillus velezensis strain SRCM102746]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363