Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   C7M22_RS20360 Genome accession   NZ_CP028208
Coordinates   4099263..4099877 (-) Length   204 a.a.
NCBI ID   WP_043021637.1    Uniprot ID   -
Organism   Bacillus velezensis strain SRCM102744     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4094263..4104877
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7M22_RS20330 (C7M22_04099) rpsT 4094566..4094832 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  C7M22_RS20335 (C7M22_04100) holA 4094849..4095890 (-) 1042 Protein_4000 DNA polymerase III subunit delta -
  C7M22_RS20340 - 4095930..4096081 (-) 152 Protein_4001 hypothetical protein -
  C7M22_RS20345 - 4096122..4096256 (+) 135 WP_003152870.1 YqzM family protein -
  C7M22_RS20350 (C7M22_04101) comEC 4096275..4098626 (-) 2352 WP_094031847.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  C7M22_RS20355 (C7M22_04102) - 4098627..4099196 (-) 570 WP_094031848.1 ComE operon protein 2 -
  C7M22_RS20360 (C7M22_04103) comEA 4099263..4099877 (-) 615 WP_043021637.1 helix-hairpin-helix domain-containing protein Machinery gene

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22165.31 Da        Isoelectric Point: 6.4888

>NTDB_id=282835 C7M22_RS20360 WP_043021637.1 4099263..4099877(-) (comEA) [Bacillus velezensis strain SRCM102744]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=282835 C7M22_RS20360 WP_043021637.1 4099263..4099877(-) (comEA) [Bacillus velezensis strain SRCM102744]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA Latilactobacillus sakei subsp. sakei 23K

32.759

100

0.373
  comEA/celA/cilE Streptococcus mitis NCTC 12261

35.545

100

0.368
  comEA/celA/cilE Streptococcus pneumoniae Rx1

35.545

100

0.368
  comEA/celA/cilE Streptococcus pneumoniae D39

35.545

100

0.368
  comEA/celA/cilE Streptococcus pneumoniae R6

35.545

100

0.368
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363

Multiple sequence alignment