Detailed information NTG0282723

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: C7M21_RS10450
• Genome accession: NZ_CP028207
• Coordinates: 2045426..2046040 (+)
• Length: 204 a.a.
• NCBI ID: WP_043021637.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain SRCM102743
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2040426..2051040

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C7M21_RS10415 (C7M21_02088)	aroE	2041113..2041955 (+)	843	WP_094031849.1	shikimate dehydrogenase	-
C7M21_RS10420 (C7M21_02089)	yhbY	2041949..2042239 (+)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
C7M21_RS10425 (C7M21_02090)	-	2042250..2042819 (+)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
C7M21_RS10430 (C7M21_02091)	yqeK	2042809..2043369 (+)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
C7M21_RS10435 (C7M21_02092)	rsfS	2043387..2043743 (+)	357	WP_007408260.1	ribosome silencing factor	-
C7M21_RS10440 (C7M21_02093)	-	2043740..2044477 (+)	738	WP_076424998.1	class I SAM-dependent DNA methyltransferase	-
C7M21_RS10445 (C7M21_02094)	comER	2044546..2045367 (-)	822	WP_012118027.1	late competence protein ComER	-
C7M21_RS10450 (C7M21_02095)	comEA	2045426..2046040 (+)	615	WP_043021637.1	helix-hairpin-helix domain-containing protein	Machinery gene
C7M21_RS10455 (C7M21_02096)	-	2046107..2046676 (+)	570	WP_094031848.1	ComE operon protein 2	-
C7M21_RS10460 (C7M21_02097)	comEC	2046677..2049028 (+)	2352	WP_094031847.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
C7M21_RS10465	-	2049047..2049181 (-)	135	WP_003152870.1	YqzM family protein	-
C7M21_RS10470	-	2049222..2049373 (+)	152	Protein_2024	hypothetical protein	-
C7M21_RS10475 (C7M21_02098)	holA	2049413..2050454 (+)	1042	Protein_2025	DNA polymerase III subunit delta	-
C7M21_RS10480 (C7M21_02099)	rpsT	2050471..2050737 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22165.31 Da
• Isoelectric Point: 6.4888

>NTDB_id=282723 C7M21_RS10450 WP_043021637.1 2045426..2046040(+) (comEA) [Bacillus velezensis strain SRCM102743]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=282723 C7M21_RS10450 WP_043021637.1 2045426..2046040(+) (comEA) [Bacillus velezensis strain SRCM102743]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363