Detailed information NTG0282657

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: C7M20_RS12415
• Genome accession: NZ_CP028206
• Coordinates: 2572098..2572712 (-)
• Length: 204 a.a.
• NCBI ID: WP_043021637.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain SRCM102742
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2567098..2577712

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C7M20_RS12385 (C7M20_02505)	rpsT	2567401..2567667 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
C7M20_RS12390 (C7M20_02506)	holA	2567684..2568725 (-)	1042	Protein_2433	DNA polymerase III subunit delta	-
C7M20_RS12395	-	2568765..2568916 (-)	152	Protein_2434	hypothetical protein	-
C7M20_RS12400	-	2568957..2569091 (+)	135	WP_003152870.1	YqzM family protein	-
C7M20_RS12405 (C7M20_02507)	comEC	2569110..2571461 (-)	2352	WP_094031847.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
C7M20_RS12410 (C7M20_02508)	-	2571462..2572031 (-)	570	WP_094031848.1	ComE operon protein 2	-
C7M20_RS12415 (C7M20_02509)	comEA	2572098..2572712 (-)	615	WP_043021637.1	helix-hairpin-helix domain-containing protein	Machinery gene
C7M20_RS12420 (C7M20_02510)	comER	2572771..2573592 (+)	822	WP_012118027.1	late competence protein ComER	-
C7M20_RS12425 (C7M20_02511)	-	2573661..2574398 (-)	738	WP_076424998.1	class I SAM-dependent DNA methyltransferase	-
C7M20_RS12430 (C7M20_02512)	rsfS	2574395..2574751 (-)	357	WP_007408260.1	ribosome silencing factor	-
C7M20_RS12435 (C7M20_02513)	yqeK	2574769..2575329 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
C7M20_RS12440 (C7M20_02514)	-	2575319..2575888 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
C7M20_RS12445 (C7M20_02515)	yhbY	2575899..2576189 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
C7M20_RS12450 (C7M20_02516)	aroE	2576183..2577025 (-)	843	WP_094031849.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22165.31 Da
• Isoelectric Point: 6.4888

>NTDB_id=282657 C7M20_RS12415 WP_043021637.1 2572098..2572712(-) (comEA) [Bacillus velezensis strain SRCM102742]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=282657 C7M20_RS12415 WP_043021637.1 2572098..2572712(-) (comEA) [Bacillus velezensis strain SRCM102742]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363