Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   C4K21_RS02330 Genome accession   NZ_CP027742
Coordinates   513282..515354 (+) Length   690 a.a.
NCBI ID   WP_124333892.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. aurantiaca strain 464     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 508282..520354
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K21_RS02310 (C4K21_0467) pilM 510512..511564 (+) 1053 WP_028683780.1 pilus assembly protein PilM Machinery gene
  C4K21_RS02315 (C4K21_0468) - 511564..512130 (+) 567 WP_124333889.1 PilN domain-containing protein -
  C4K21_RS02320 (C4K21_0469) pilO 512127..512744 (+) 618 WP_124333890.1 type 4a pilus biogenesis protein PilO -
  C4K21_RS02325 (C4K21_0470) - 512741..513268 (+) 528 WP_124333891.1 pilus assembly protein PilP -
  C4K21_RS02330 (C4K21_0471) pilQ 513282..515354 (+) 2073 WP_124333892.1 type IV pilus secretin PilQ Machinery gene
  C4K21_RS02335 (C4K21_0472) aroK 515359..515877 (+) 519 WP_009041824.1 shikimate kinase AroK -
  C4K21_RS02340 (C4K21_0473) aroB 516025..517125 (+) 1101 WP_016704447.1 3-dehydroquinate synthase -
  C4K21_RS02345 (C4K21_0474) - 517136..518725 (+) 1590 WP_016704448.1 AAA family ATPase -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74644.71 Da        Isoelectric Point: 5.4337

>NTDB_id=279856 C4K21_RS02330 WP_124333892.1 513282..515354(+) (pilQ) [Pseudomonas chlororaphis subsp. aurantiaca strain 464]
MNRIFSAFGIALWMALLSPMAMAASLKALDVAALPGDRLELKLTFDGPVPEPRGYTTDQPARIALDLPGVVSQLANKSRD
LGNGNARSVTVVESGQRTRVVVNLVALAPYSTRVAGNQLFVVIGQGATAAQSAPGQPAVASGRAKAAASAGRSIRSVDFQ
RGEQGEGNVLIDLSDSGMSPDIQERDGKLVLTFAKARLPEPLRVRLDVKDFATPVQFVSARAESGKAIVTIEPGGTFDYS
TYQTDNKLTVSVRPMTSGDLQKRNAERSAYNGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQ
ALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLEPLRRELLQVNYAKASEIAKLFQSVTRAEDKSDER
GSITVDERTNNIIAYQTRERLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNQGNWNASGVSNGTNG
SSTIGTPGSTSSNSPFVDLGTTANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
AASSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLINDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=279856 C4K21_RS02330 WP_124333892.1 513282..515354(+) (pilQ) [Pseudomonas chlororaphis subsp. aurantiaca strain 464]
ATGAACAGGATCTTTTCAGCCTTCGGTATTGCGCTATGGATGGCGCTGCTTTCACCGATGGCCATGGCGGCCAGCCTCAA
GGCCCTGGATGTCGCGGCACTGCCGGGGGATCGCCTGGAGTTGAAACTCACCTTCGATGGGCCGGTGCCCGAGCCCCGGG
GTTACACCACCGACCAGCCGGCGCGGATCGCCCTCGATCTGCCGGGTGTCGTCAGCCAGCTGGCGAACAAGAGCCGCGAT
CTGGGCAATGGCAACGCTCGCAGCGTGACAGTGGTCGAATCGGGACAGCGTACCCGGGTGGTGGTCAACCTGGTCGCGCT
GGCGCCCTACAGTACTCGAGTTGCCGGCAATCAGCTGTTTGTGGTGATCGGCCAGGGGGCGACGGCGGCGCAGAGTGCTC
CGGGGCAGCCGGCTGTCGCATCCGGGCGGGCCAAGGCTGCGGCTTCGGCGGGGCGGTCGATTCGCAGTGTGGATTTTCAG
CGCGGCGAGCAGGGTGAGGGCAATGTACTGATCGACCTCTCCGACTCGGGAATGAGCCCGGATATCCAGGAACGCGACGG
CAAGCTCGTCCTCACCTTTGCCAAGGCCCGCCTGCCAGAGCCCCTGCGGGTCAGGCTGGACGTCAAGGACTTCGCCACTC
CCGTGCAATTCGTCAGCGCCCGGGCGGAGTCCGGCAAGGCCATCGTCACCATCGAGCCCGGCGGCACCTTCGATTACTCG
ACCTACCAGACGGACAACAAGCTCACGGTAAGCGTCAGGCCCATGACTTCGGGCGATCTGCAGAAACGCAATGCCGAGCG
CTCGGCCTATAACGGCGAAAAGCTCTCGCTGAACTTCCAGGACATCGAGGTCCGCTCGGTGCTGCAACTGATCGCCGACT
TCACCAACCTCAATCTGGTAGCCAGCGACACGGTGCAGGGTGGCATTACCTTGCGCCTGCAGAATGTGCCGTGGGACCAG
GCCCTCGATCTGGTGCTCAAGACCAAGGGGCTGGACAAACGTAAGGTGGGTAATGTGTTGCTGGTGGCGCCAGCCGATGA
AATCGCCGCCCGCGAACGCCAGGAGCTGGAGTCGCAGAAGCAGATAGCCGACCTGGAACCGCTGCGACGCGAACTGCTGC
AGGTCAACTACGCCAAGGCCTCGGAGATCGCCAAGTTGTTCCAGTCGGTCACCCGCGCCGAGGACAAGTCCGACGAGCGT
GGCTCGATCACGGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACCCGGGAGCGCCTGGACGAGCTGCGGCGGAT
TGTCAGCCAGCTGGATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCGACTACGACAAAA
GCCTGGGCGTGCGCTGGGGTGGTTCGATCCAGAACCAGGGCAACTGGAATGCCTCGGGGGTCAGCAACGGCACCAACGGC
TCTTCCACCATAGGCACGCCAGGCAGTACCAGCAGCAACTCACCGTTCGTCGACCTGGGCACCACCGCCAATACCTCCGG
CCTGGGTATCGCCTTCATCACCGACAACGTGTTGCTGGACCTTGAGCTGACAGCCATGGAAAAGACTGGTAACGGGGAAA
TCGTCTCCCAGCCCAAGGTGGTCACCTCCGACAAAGAGACGGCGAAGATTCTCAAGGGTACGGAAATCCCCTATCAGGAA
GCCGCCTCCAGCGGGGCGACTTCGGTGTCGTTCAAGGAGGCGTCGCTGTCCCTGGAGGTGACGCCGCAGATCACCCCGGA
TAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTGAACAAGGTCCAGGACGTGCCGCCGATCA
AGAAGAACGAGGTCAACGCCAAGGTCCTGATCAACGACGGAGAAACCATCGTTATCGGTGGGGTTTTCTCCAATACGCAA
AGCAAGGTTGTAGATAAGGTGCCATTTCTAGGCGATGTGCCGTATCTTGGCCGCCTTTTCAGGCGTGACGTGGTGTCGGA
GAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(40-122)

Bacterial type II and III secretion system protein (Secretin)

(525-681)

Bacterial type II/III secretion system short domain (Secretin_N)

(372-437)

Secretin and TonB N terminus short domain (STN)

(298-344)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.769

100

0.733
  comQ Acinetobacter baylyi ADP1

39.918

100

0.422
  pilQ Acinetobacter baumannii strain A118

37.987

100

0.41
  pilQ Acinetobacter baumannii D1279779

37.987

100

0.41
  pilQ Legionella pneumophila strain Lp02

37.89

97.536

0.37
  pilQ Legionella pneumophila strain ERS1305867

37.967

96.957

0.368

Multiple sequence alignment