Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PST_RS05385 Genome accession   NC_009434
Coordinates   1164577..1166280 (+) Length   567 a.a.
NCBI ID   WP_011912243.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri A1501     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1159577..1171280
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PST_RS05370 (PST_1051) cysN 1160015..1161913 (+) 1899 WP_011912239.1 sulfate adenylyltransferase subunit CysN -
  PST_RS21170 (PST_1052) - 1162246..1162800 (-) 555 WP_011912240.1 REP-associated tyrosine transposase -
  PST_RS21175 (PST_1054) - 1163755..1164267 (-) 513 WP_011912242.1 pilin -
  PST_RS05385 (PST_1055) pilB 1164577..1166280 (+) 1704 WP_011912243.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PST_RS05390 (PST_1056) pilC 1166282..1167499 (+) 1218 WP_011912244.1 type II secretion system F family protein Machinery gene
  PST_RS05395 (PST_1057) pilD 1167502..1168371 (+) 870 WP_011912245.1 prepilin peptidase Machinery gene
  PST_RS21180 (PST_1058) coaE 1168503..1169111 (+) 609 WP_080505042.1 dephospho-CoA kinase -
  PST_RS21185 yacG 1169108..1169305 (+) 198 WP_041771656.1 DNA gyrase inhibitor YacG -
  PST_RS05405 (PST_1059) - 1169318..1169998 (-) 681 WP_011912247.1 energy-coupling factor ABC transporter permease -
  PST_RS05410 (PST_1060) - 1170055..1171095 (-) 1041 WP_011912248.1 NADP(H)-dependent aldo-keto reductase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62001.00 Da        Isoelectric Point: 5.3550

>NTDB_id=27962 PST_RS05385 WP_011912243.1 1164577..1166280(+) (pilB) [Stutzerimonas stutzeri A1501]
MSENISLSGLARQMVVAGVLDERTAQQAQAQAIRSKTPFVTYVVQNKLAKGRAIAELASDQFGIALLDLSALDKESLPKD
LISEKLARQHRVLPLSRRGTKLYVAISDPANHQSIADIQFSTGLSIESILAEDDKLGEAIDRLYESGTTGLEELGEVDLD
GVDVETVNEDGKAEEGGEAADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKAYRVRLRTDGILHEVARPPIQLAPRISAR
LKVMAGLDISERRKPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEENQKELYMNALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPKQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVAAFNIATSVNLIIAQRLARKLCNSCKKEIDLPREALLEEGFP
ADKIGTFKIYGPVGCDNCKGGYKGRVGIYEVVKNTPALARIIMEDGNSIDISTQMRKDGFNDLRTSALLKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=27962 PST_RS05385 WP_011912243.1 1164577..1166280(+) (pilB) [Stutzerimonas stutzeri A1501]
ATGAGCGAAAACATTTCCCTATCCGGGCTGGCACGGCAGATGGTCGTGGCCGGCGTGCTGGACGAAAGAACAGCCCAGCA
GGCGCAGGCGCAGGCCATCCGCAGCAAAACGCCCTTTGTCACCTATGTAGTGCAGAACAAGCTGGCCAAAGGCCGCGCCA
TAGCGGAGCTTGCGTCCGACCAATTCGGTATCGCTTTGCTGGATTTGTCCGCGCTGGATAAGGAGTCGCTGCCCAAGGAC
CTGATCAGCGAGAAGCTGGCGCGGCAGCACCGTGTGCTGCCGCTTTCCAGGCGCGGCACCAAGCTCTATGTGGCCATTTC
CGATCCGGCCAATCACCAGTCGATTGCCGATATCCAGTTCAGTACCGGTCTGAGCATCGAGTCGATCCTGGCGGAAGACG
ACAAGCTGGGCGAGGCCATCGACAGGCTGTACGAAAGCGGCACCACCGGGCTAGAAGAACTGGGCGAGGTCGATCTGGAT
GGTGTCGATGTCGAGACCGTCAATGAGGATGGAAAAGCCGAGGAGGGCGGAGAGGCTGCCGACGACGCCCCCGTGGTCCG
CTTCGTCAACAAGATGCTGCTCGACGCCATTCGCGGCGGCTCGTCCGACTTGCACTTCGAGCCCTACGAAAAAGCCTACC
GCGTGCGGCTTCGCACGGACGGCATCCTGCATGAAGTCGCCCGCCCACCGATCCAGCTGGCGCCCCGCATATCCGCACGT
TTGAAGGTGATGGCCGGGCTGGACATCTCCGAGCGCCGCAAGCCGCAGGATGGCCGGATCAAGATGAAGCTGTCCAAAAC
CAAAGCCATCGACTTCCGCGTTAACACCCTGCCGACGCTGTGGGGTGAGAAGATCGTGATGCGAATTCTCGATCCGTCCA
GTGCGCAGATGGGGATCGATGCCCTGGGCTACGAAGAAAACCAGAAAGAGCTGTACATGAATGCCCTCAAGCAGCCGCAG
GGCATGATTCTGGTAACCGGCCCTACGGGATCGGGCAAGACGGTATCTCTTTACACCGGTCTCAATATTCTCAACACGGT
GGATGTGAACATCTCGACCGCCGAAGACCCGGTGGAGATCAACCTGGAGGGTATCAACCAGGTCAACGTCAACCCGAAGC
AGGGTATGGATTTCTCCCAGGCGCTACGCGCGTTTCTGCGTCAGGACCCGGACATCATCATGGTGGGCGAGATTCGCGAC
CTGGAAACAGCCGAAATTGCCATCAAGGCGGCCCAGACGGGCCACATGGTTATGTCGACGCTACACACCAACAGCGCCGC
GGAAACCCTGACCCGTCTTCGCAACATGGGGGTGGCCGCGTTCAATATTGCGACCTCGGTCAACCTGATCATCGCGCAGC
GACTGGCACGCAAGCTTTGCAACAGTTGCAAGAAGGAGATCGATCTGCCGCGCGAGGCTCTGTTGGAAGAGGGCTTCCCC
GCCGACAAGATTGGAACCTTCAAGATCTATGGTCCGGTTGGTTGCGACAATTGCAAAGGCGGTTACAAAGGTCGTGTCGG
TATTTATGAAGTGGTTAAAAACACGCCAGCGTTGGCCAGGATTATCATGGAAGACGGTAATTCGATCGATATTTCCACCC
AGATGCGAAAAGATGGCTTCAATGACCTGCGCACCTCGGCTTTGCTCAAGGCCATGCAGGGCGTGACCAGCCTGGAAGAA
GTCAACCGGGTGACCAAGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.752

99.471

0.594
  pilB Acinetobacter baylyi ADP1

59.503

99.295

0.591
  pilB Legionella pneumophila strain ERS1305867

54.737

100

0.55
  pilB Vibrio cholerae strain A1552

51.648

96.296

0.497
  pilB Vibrio campbellii strain DS40M4

49.298

100

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

52.692

91.711

0.483
  pilF Neisseria gonorrhoeae MS11

51.154

91.711

0.469

Multiple sequence alignment