Detailed information NTG0027964

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PST_RS05395
• Genome accession: NC_009434
• Coordinates: 1167502..1168371 (+)
• Length: 289 a.a.
• NCBI ID: WP_011912245.1
• Uniprot ID: A4VIF3
• Organism: Stutzerimonas stutzeri A1501
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1162502..1173371

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PST_RS21175 (PST_1054)	-	1163755..1164267 (-)	513	WP_011912242.1	pilin	-
PST_RS05385 (PST_1055)	pilB	1164577..1166280 (+)	1704	WP_011912243.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PST_RS05390 (PST_1056)	pilC	1166282..1167499 (+)	1218	WP_011912244.1	type II secretion system F family protein	Machinery gene
PST_RS05395 (PST_1057)	pilD	1167502..1168371 (+)	870	WP_011912245.1	prepilin peptidase	Machinery gene
PST_RS21180 (PST_1058)	coaE	1168503..1169111 (+)	609	WP_080505042.1	dephospho-CoA kinase	-
PST_RS21185	yacG	1169108..1169305 (+)	198	WP_041771656.1	DNA gyrase inhibitor YacG	-
PST_RS05405 (PST_1059)	-	1169318..1169998 (-)	681	WP_011912247.1	energy-coupling factor ABC transporter permease	-
PST_RS05410 (PST_1060)	-	1170055..1171095 (-)	1041	WP_011912248.1	NADP(H)-dependent aldo-keto reductase	-
PST_RS05415 (PST_1061)	rplM	1171510..1171938 (+)	429	WP_003287302.1	50S ribosomal protein L13	-
PST_RS05420 (PST_1062)	rpsI	1171954..1172346 (+)	393	WP_011912249.1	30S ribosomal protein S9	-
PST_RS05425 (PST_1063)	petA	1172601..1173194 (+)	594	WP_011912250.1	ubiquinol-cytochrome c reductase iron-sulfur subunit	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32093.02 Da
• Isoelectric Point: 7.2758

>NTDB_id=27964 PST_RS05395 WP_011912245.1 1167502..1168371(+) (pilD) [Stutzerimonas stutzeri A1501]
MGLIDFLASHVLAFVLSAAVLGLLVGSFLNVVIYRVPIMMQREWRMQAREYLEYPPEPIGERFNLLVPSSRCPHCGHRIR
AWENIPLVSWLALRGKCSSCQAPISCRYPLVELACGVLSGYVAWHFGFSWQAGAMLLLTWGLLAMSMIDVDHQLLPDSLV
LPLLWLGLIANSFALFASLEDALWGAIAGYLSLWSVYWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQVLPLTILLSSVVG
AVLGSILLRVQRAESSTPIPFGPYLAIAGWIALLWGDRITESYLQFARF

Nucleotide

• Length: 870 bp

>NTDB_id=27964 PST_RS05395 WP_011912245.1 1167502..1168371(+) (pilD) [Stutzerimonas stutzeri A1501]
ATGGGTTTGATTGATTTCTTGGCCAGCCATGTGCTGGCCTTTGTTTTGTCTGCTGCAGTGCTCGGTCTGCTGGTTGGCAG
CTTTCTCAATGTCGTCATCTACCGTGTGCCGATCATGATGCAGCGCGAATGGCGCATGCAGGCGCGGGAATACTTGGAGT
ATCCGCCCGAGCCAATTGGGGAGCGTTTCAATCTACTGGTGCCGAGTTCACGCTGCCCTCACTGCGGTCATCGTATCCGT
GCCTGGGAGAACATTCCCTTGGTCAGCTGGCTGGCGCTGCGTGGAAAGTGCTCGTCTTGTCAGGCGCCGATCAGCTGTCG
ATATCCGTTGGTTGAGCTGGCGTGTGGCGTGCTATCCGGATACGTGGCATGGCATTTCGGTTTTTCCTGGCAGGCCGGGG
CGATGCTTCTGCTGACCTGGGGGTTGCTGGCGATGAGCATGATCGATGTCGATCATCAGTTGCTGCCTGACTCGCTCGTG
CTGCCGCTCTTGTGGTTAGGGTTGATTGCCAACAGCTTTGCTCTGTTCGCCTCTCTCGAGGATGCGCTGTGGGGGGCGAT
AGCCGGTTACCTGAGCCTATGGTCCGTTTATTGGCTGTTCAAGCTGGTCACAGGCAAAGAGGGTATGGGCTACGGCGATT
TCAAGCTGCTGGCGATGCTTGGTGCCTGGGGCGGCTGGCAGGTTCTGCCCCTCACTATCTTGCTGTCTTCGGTGGTCGGC
GCCGTTCTTGGCAGTATTTTGCTGAGGGTGCAGCGGGCTGAAAGCAGTACGCCCATTCCGTTCGGGCCGTATCTGGCGAT
AGCGGGGTGGATTGCGTTGCTTTGGGGCGACCGAATTACCGAAAGCTACCTCCAGTTCGCTCGCTTCTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (137-244)

3D structure

• AlphaFold DB: A4VIF3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	57.818	95.156	0.55
pilD	Vibrio campbellii strain DS40M4	57.664	94.81	0.547
pilD	Acinetobacter baumannii D1279779	50.704	98.27	0.498
pilD	Acinetobacter nosocomialis M2	50.704	98.27	0.498
pilD	Neisseria gonorrhoeae MS11	53.612	91.003	0.488