Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   C4K15_RS02325 Genome accession   NZ_CP027718
Coordinates   512424..514496 (+) Length   690 a.a.
NCBI ID   WP_124337586.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. aurantiaca strain Q16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 507424..519496
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K15_RS02305 (C4K15_0462) pilM 509654..510706 (+) 1053 WP_124337583.1 pilus assembly protein PilM Machinery gene
  C4K15_RS02310 (C4K15_0463) - 510706..511272 (+) 567 WP_124298333.1 PilN domain-containing protein -
  C4K15_RS02315 (C4K15_0464) pilO 511269..511886 (+) 618 WP_124337584.1 type 4a pilus biogenesis protein PilO -
  C4K15_RS02320 (C4K15_0465) - 511883..512410 (+) 528 WP_124337585.1 pilus assembly protein PilP -
  C4K15_RS02325 (C4K15_0466) pilQ 512424..514496 (+) 2073 WP_124337586.1 type IV pilus secretin PilQ Machinery gene
  C4K15_RS02330 (C4K15_0467) aroK 514501..515019 (+) 519 WP_009041824.1 shikimate kinase AroK -
  C4K15_RS02335 (C4K15_0468) aroB 515168..516268 (+) 1101 WP_016704447.1 3-dehydroquinate synthase -
  C4K15_RS02340 (C4K15_0469) - 516279..517868 (+) 1590 WP_124337587.1 AAA family ATPase -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74688.76 Da        Isoelectric Point: 5.4337

>NTDB_id=279248 C4K15_RS02325 WP_124337586.1 512424..514496(+) (pilQ) [Pseudomonas chlororaphis subsp. aurantiaca strain Q16]
MNRIFSAFGIALWMALLSPMAMAASLKALDVAALPGDRLELKLTFDGPVPEPRGYTTDQPARIALDLPGVVSQLTNKSRD
LGNGNARSVTVVESGQRTRVVVNLVALAPYSTRVAGNQLFVVIGQGATAAQSAPGQPAVASGRAKAAASAGRSIRSVDFQ
RGEQGEGNVLIDLSDSGMSPDIQERDGKLVLTFAKARLPEPLRVRLDVKDFATPVQFVSARAESGKAIVTIEPGGTFDYS
TYQTDNKLTVSVRPMTSGDLQKRNTERSAYNGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQ
ALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLEPLRRELLQVNYAKAAEIAKLFQSVTRAEDKSDER
GSITVDERTNNIIAYQTRERLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNQGNWNASGVSNGTNG
SSTIGTPGSTSSNSPFVDLGTTANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
AASSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLINDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=279248 C4K15_RS02325 WP_124337586.1 512424..514496(+) (pilQ) [Pseudomonas chlororaphis subsp. aurantiaca strain Q16]
ATGAACAGGATCTTTTCAGCCTTCGGTATTGCGCTATGGATGGCGCTGCTTTCACCGATGGCCATGGCGGCCAGCCTCAA
GGCCCTGGATGTCGCGGCACTGCCGGGGGATCGCCTGGAGTTGAAACTCACCTTCGATGGGCCGGTGCCCGAGCCGCGGG
GTTACACCACCGACCAGCCGGCGCGGATCGCCCTCGATCTGCCGGGTGTCGTCAGCCAGCTGACGAACAAGAGCCGCGAT
CTGGGCAATGGCAATGCACGCAGCGTGACAGTAGTCGAATCGGGGCAGCGTACCCGGGTGGTGGTCAACCTGGTCGCGCT
GGCGCCCTACAGTACTCGAGTTGCCGGCAATCAGCTGTTTGTGGTGATCGGCCAGGGGGCGACGGCGGCGCAGAGTGCTC
CGGGGCAGCCGGCTGTCGCATCCGGGCGGGCCAAGGCTGCGGCTTCGGCGGGGCGGTCGATTCGCAGCGTGGATTTTCAG
CGCGGCGAGCAGGGTGAGGGCAATGTACTGATCGACCTCTCCGACTCGGGGATGAGCCCGGATATCCAGGAACGCGACGG
CAAGCTCGTCCTCACCTTCGCCAAGGCCCGCCTGCCAGAGCCCCTGCGGGTCAGGCTGGACGTCAAGGACTTCGCCACTC
CCGTGCAATTCGTCAGCGCCCGGGCGGAGTCCGGCAAGGCCATCGTCACCATCGAGCCCGGCGGCACCTTCGATTACTCG
ACCTACCAGACGGACAACAAGCTCACGGTAAGTGTCAGGCCGATGACTTCGGGCGATCTGCAGAAACGCAACACCGAGCG
CTCGGCCTATAACGGCGAAAAGCTCTCGCTGAACTTCCAGGACATCGAGGTCCGCTCGGTGCTGCAACTGATCGCCGACT
TCACCAACCTCAACCTGGTGGCCAGCGATACGGTGCAGGGCGGCATTACCTTGCGCCTGCAGAATGTGCCGTGGGACCAG
GCCCTCGATCTGGTGCTCAAGACCAAGGGGTTGGACAAGCGTAAGGTGGGTAATGTGTTGCTGGTGGCGCCGGCCGATGA
AATCGCCGCCCGCGAACGCCAGGAGCTGGAGTCGCAGAAGCAGATAGCCGACCTGGAACCGCTGCGGCGCGAACTGCTGC
AGGTCAACTACGCCAAGGCCGCGGAAATCGCCAAGTTGTTCCAGTCGGTCACCCGCGCCGAGGACAAGTCCGACGAGCGT
GGTTCGATCACGGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACCCGGGAGCGCCTGGACGAGCTGCGGCGGAT
TGTCAGCCAGCTGGATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCGACTACGACAAAA
GCCTGGGCGTGCGCTGGGGTGGTTCGATCCAGAACCAGGGCAACTGGAATGCCTCGGGGGTCAGCAACGGCACCAACGGC
TCTTCCACCATAGGCACGCCAGGCAGTACCAGCAGCAACTCACCGTTCGTCGACCTGGGCACCACCGCCAATACCTCCGG
CCTAGGTATCGCCTTCATCACCGACAACGTGTTGCTGGACCTTGAACTGACAGCCATGGAAAAGACCGGTAACGGGGAAA
TCGTCTCCCAGCCCAAGGTGGTCACCTCCGACAAAGAGACGGCGAAGATTCTCAAGGGTACGGAAATCCCCTATCAGGAA
GCCGCCTCCAGCGGGGCGACTTCGGTGTCGTTCAAGGAGGCGTCGCTGTCCCTGGAGGTGACGCCGCAGATCACCCCGGA
TAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTGAACAAGGTCCAGGACGTGCCGCCGATCA
AGAAGAACGAGGTCAACGCCAAGGTCCTGATCAACGACGGAGAAACCATCGTTATCGGTGGGGTTTTCTCCAATACGCAA
AGCAAGGTTGTAGATAAGGTGCCATTTCTAGGCGATGTGCCGTATCTTGGCCGCCTTTTCAGGCGTGACGTGGTGTCGGA
GAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(372-437)

Secretin and TonB N terminus short domain (STN)

(298-344)

Bacterial type II and III secretion system protein (Secretin)

(525-681)

AMIN domain (AMIN)

(40-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.909

100

0.735
  comQ Acinetobacter baylyi ADP1

39.918

100

0.422
  pilQ Acinetobacter baumannii strain A118

37.987

100

0.41
  pilQ Acinetobacter baumannii D1279779

37.987

100

0.41
  pilQ Legionella pneumophila strain Lp02

38.039

97.536

0.371
  pilQ Legionella pneumophila strain ERS1305867

38.117

96.957

0.37

Multiple sequence alignment