Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   C4K16_RS02355 Genome accession   NZ_CP027717
Coordinates   522701..524773 (+) Length   690 a.a.
NCBI ID   WP_124335974.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. aurantiaca strain PCM 2210     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 517701..529773
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K16_RS02335 (C4K16_0464) pilM 519931..520983 (+) 1053 WP_028683780.1 pilus assembly protein PilM Machinery gene
  C4K16_RS02340 (C4K16_0465) - 520983..521549 (+) 567 WP_124333889.1 PilN domain-containing protein -
  C4K16_RS02345 (C4K16_0466) pilO 521546..522163 (+) 618 WP_124335972.1 type 4a pilus biogenesis protein PilO -
  C4K16_RS02350 (C4K16_0467) - 522160..522687 (+) 528 WP_124335973.1 pilus assembly protein PilP -
  C4K16_RS02355 (C4K16_0468) pilQ 522701..524773 (+) 2073 WP_124335974.1 type IV pilus secretin PilQ Machinery gene
  C4K16_RS02360 (C4K16_0469) aroK 524778..525296 (+) 519 WP_009041824.1 shikimate kinase AroK -
  C4K16_RS02365 (C4K16_0470) aroB 525444..526544 (+) 1101 WP_016704447.1 3-dehydroquinate synthase -
  C4K16_RS02370 (C4K16_0471) - 526555..528144 (+) 1590 WP_016704448.1 AAA family ATPase -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74658.74 Da        Isoelectric Point: 5.4337

>NTDB_id=279221 C4K16_RS02355 WP_124335974.1 522701..524773(+) (pilQ) [Pseudomonas chlororaphis subsp. aurantiaca strain PCM 2210]
MNRIFSAFGIALWMALLSPMAMAASLKALDVAALPGDRLELKLTFDGPVPEPRGYTTDQPARIALDLPGVVSQLTNKSRD
LGNGNARSVTVVESGQRTRVVVNLVALAPYSTRVAGNQLFVVIGQGATAAQSAPGQPAVASGRAKAVASAGRSIRSVDFQ
RGEQGEGNVLIDLSDSGMSPDIQERDGKLALTFAKARLPEPLRVRLDVKDFATPVQFVSARAESGKAIVTIEPGGTFDYS
TYQTDNKLTVSVRPMTSGDLQKRNAERSAYNGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQ
ALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLEPLRRELLQVNYAKAAEIAKLFQSVTRAEDKSDER
GSITVDERTNNIIAYQTRERLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNQGNWNASGVSNGTNG
SSTIGTPGSTSSNSPFVDLGTTANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
AASSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLINDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=279221 C4K16_RS02355 WP_124335974.1 522701..524773(+) (pilQ) [Pseudomonas chlororaphis subsp. aurantiaca strain PCM 2210]
ATGAACAGGATCTTTTCAGCCTTCGGTATTGCGCTATGGATGGCGCTGCTTTCACCGATGGCCATGGCGGCCAGCCTCAA
GGCCCTGGATGTCGCGGCACTGCCGGGGGATCGCCTGGAGTTGAAACTCACCTTCGATGGGCCGGTGCCCGAGCCCCGGG
GTTACACCACCGACCAGCCGGCGCGGATCGCCCTCGATCTGCCGGGTGTCGTCAGCCAGCTGACGAACAAGAGCCGCGAT
CTGGGCAATGGCAATGCACGCAGCGTGACAGTAGTCGAATCGGGGCAGCGTACCCGGGTGGTGGTCAACCTGGTCGCGCT
GGCGCCCTACAGTACTCGAGTTGCCGGCAATCAGCTGTTTGTGGTGATTGGCCAGGGGGCGACAGCGGCGCAGAGTGCTC
CGGGGCAGCCGGCTGTCGCATCCGGGCGGGCCAAGGCTGTGGCTTCGGCGGGGCGGTCGATTCGCAGCGTGGATTTTCAG
CGCGGCGAGCAGGGTGAGGGCAACGTACTGATCGACCTCTCCGACTCGGGAATGAGCCCGGATATCCAGGAGCGCGACGG
CAAGCTCGCCCTTACCTTCGCCAAGGCCCGCCTGCCAGAGCCCCTGCGGGTCAGGCTGGACGTCAAGGACTTCGCCACCC
CCGTGCAATTCGTCAGCGCCCGGGCGGAGTCCGGCAAGGCCATCGTCACCATCGAGCCCGGCGGCACCTTCGATTACTCG
ACCTACCAGACGGACAACAAGCTCACGGTAAGCGTCAGGCCCATGACTTCGGGCGATCTGCAGAAACGCAATGCCGAGCG
CTCGGCCTATAACGGCGAAAAGCTCTCGCTGAACTTCCAGGACATCGAGGTCCGCTCGGTGCTGCAACTGATCGCCGACT
TCACCAACCTCAACCTGGTGGCCAGCGACACGGTGCAGGGTGGCATTACCTTGCGCCTGCAGAATGTGCCGTGGGACCAG
GCCCTCGATCTGGTGCTCAAGACCAAGGGGCTGGACAAGCGCAAGGTGGGCAATGTGTTGCTGGTGGCGCCGGCCGACGA
AATCGCCGCCCGCGAACGCCAGGAGCTGGAGTCGCAGAAGCAGATCGCCGACCTGGAACCGCTGCGACGCGAACTGCTGC
AGGTCAACTACGCCAAGGCCGCGGAGATCGCCAAGTTGTTCCAGTCGGTCACCCGCGCCGAGGACAAGTCCGACGAGCGT
GGGTCGATCACGGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACCCGGGAGCGCCTGGACGAGCTGCGGCGGAT
TGTCAGCCAGCTGGATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCGACTACGACAAAA
GCCTGGGCGTGCGCTGGGGTGGTTCGATCCAGAACCAGGGCAACTGGAATGCCTCGGGGGTCAGCAACGGCACCAACGGC
TCTTCCACCATAGGCACGCCAGGCAGTACCAGCAGCAACTCACCGTTCGTCGACCTGGGCACCACCGCCAATACCTCCGG
CCTGGGTATCGCCTTCATCACTGACAACGTGTTGCTGGACCTTGAACTGACAGCCATGGAAAAGACCGGTAACGGGGAAA
TCGTCTCCCAGCCCAAGGTCGTCACGTCCGACAAGGAGACGGCGAAGATTCTCAAGGGTACGGAAATCCCCTATCAGGAA
GCGGCCTCCAGCGGGGCGACTTCGGTGTCGTTCAAGGAGGCGTCGCTGTCCCTGGAGGTGACGCCGCAGATCACCCCGGA
TAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTGAACAAGGTCCAGGATGTGCCGCCGATCA
AGAAGAACGAGGTCAACGCCAAGGTCCTGATCAACGACGGAGAAACCATCGTTATCGGTGGGGTTTTCTCCAATACGCAA
AGCAAGGTTGTAGATAAGGTGCCATTTCTAGGCGATGTGCCGTATCTTGGCCGCCTTTTCAGGCGTGACGTGGTGTCGGA
GAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(372-437)

AMIN domain (AMIN)

(40-122)

Secretin and TonB N terminus short domain (STN)

(298-344)

Bacterial type II and III secretion system protein (Secretin)

(525-681)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.909

100

0.735
  comQ Acinetobacter baylyi ADP1

39.781

100

0.42
  pilQ Acinetobacter baumannii D1279779

37.987

100

0.41
  pilQ Acinetobacter baumannii strain A118

37.987

100

0.41
  pilQ Legionella pneumophila strain Lp02

38.039

97.536

0.371
  pilQ Legionella pneumophila strain ERS1305867

38.117

96.957

0.37

Multiple sequence alignment