Detailed information NTG0027871

Bioinformatically predicted

Overview

• Name: ciaR
• Type: Regulator
• Locus tag: ECDH10B_RS16255
• Genome accession: NC_010473
• Coordinates: 3265595..3266254 (+)
• Length: 219 a.a.
• NCBI ID: WP_001221495.1
• Uniprot ID: P52076
• Organism: Escherichia coli str. K-12 substr. DH10B
• Function: repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Genomic Context

Location: 3260595..3271254

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ECDH10B_RS16230 (ECDH10B_3194)	ygiS	3261878..3263485 (-)	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
ECDH10B_RS16235 (ECDH10B_3195)	mqsA	3263618..3264013 (-)	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	-
ECDH10B_RS16240 (ECDH10B_3196)	mqsR	3264015..3264311 (-)	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	-
ECDH10B_RS16245 (ECDH10B_3197)	ygiV	3264516..3264998 (-)	483	WP_000183505.1	GyrI-like domain-containing protein	-
ECDH10B_RS16250 (ECDH10B_3198)	ygiW	3265051..3265443 (-)	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
ECDH10B_RS16255 (ECDH10B_3199)	ciaR	3265595..3266254 (+)	660	WP_001221495.1	quorum sensing response regulator transcription factor QseB	Regulator
ECDH10B_RS16260 (ECDH10B_3200)	qseC	3266251..3267600 (+)	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
ECDH10B_RS16265 (ECDH10B_3201)	ygiZ	3267646..3267978 (-)	333	WP_000917684.1	DUF2645 family protein	-
ECDH10B_RS16270 (ECDH10B_3202)	mdaB	3268297..3268878 (+)	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
ECDH10B_RS16275 (ECDH10B_3203)	ygiN	3268909..3269223 (+)	315	WP_000958598.1	putative quinol monooxygenase	-
ECDH10B_RS16280 (ECDH10B_3204)	parE	3269271..3271163 (-)	1893	WP_000195296.1	DNA topoisomerase IV subunit B	-

Sequence

Protein

• Length: 219 a.a.
• Molecular weight: 24677.59 Da
• Isoelectric Point: 6.9850

>NTDB_id=27871 ECDH10B_RS16255 WP_001221495.1 3265595..3266254(+) (ciaR) [Escherichia coli str. K-12 substr. DH10B]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLSRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide

• Length: 660 bp

>NTDB_id=27871 ECDH10B_RS16255 WP_001221495.1 3265595..3266254(+) (ciaR) [Escherichia coli str. K-12 substr. DH10B]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGTCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

• Response regulator receiver domain (Response_reg) (3-112)

• Transcriptional regulatory protein, C terminal (Trans_reg_C) (145-216)

3D structure

• AlphaFold DB: P52076

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ciaR	Streptococcus pneumoniae Rx1	37.885	100	0.393
ciaR	Streptococcus pneumoniae D39	37.885	100	0.393
ciaR	Streptococcus pneumoniae R6	37.885	100	0.393
ciaR	Streptococcus pneumoniae TIGR4	37.885	100	0.393
ciaR	Streptococcus mutans UA159	35.426	100	0.361