Detailed information NTG0276767

Bioinformatically predicted

Overview

• Name: pilE
• Type: Machinery gene
• Locus tag: PA34_RS26100
• Genome accession: NZ_CP032552
• Coordinates: 5441678..5442217 (-)
• Length: 179 a.a.
• NCBI ID: WP_003141352.1
• Uniprot ID: A0A0H2ZFW8
• Organism: Pseudomonas aeruginosa strain PA34
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5436678..5447217

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PA34_RS26055 (PA34_026055)	-	5436987..5437235 (+)	249	WP_062832576.1	hypothetical protein	-
PA34_RS26060 (PA34_026060)	-	5437210..5437656 (+)	447	WP_062832577.1	hypothetical protein	-
PA34_RS26065 (PA34_026065)	-	5437882..5438598 (+)	717	WP_062832578.1	hypothetical protein	-
PA34_RS26070 (PA34_026070)	-	5438591..5438866 (+)	276	WP_050714322.1	hypothetical protein	-
PA34_RS26075 (PA34_026075)	-	5439084..5439551 (+)	468	WP_062832579.1	hypothetical protein	-
PA34_RS26080 (PA34_026080)	-	5439545..5439925 (+)	381	WP_062832580.1	hypothetical protein	-
PA34_RS26085 (PA34_026085)	-	5440016..5440483 (-)	468	WP_062832581.1	hypothetical protein	-
PA34_RS26095 (PA34_026095)	-	5440917..5441615 (-)	699	WP_016254081.1	hypothetical protein	-
PA34_RS26100 (PA34_026100)	pilE	5441678..5442217 (-)	540	WP_003141352.1	pilin	Machinery gene
PA34_RS26105 (PA34_026105)	pilB	5442446..5444149 (+)	1704	WP_003141354.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PA34_RS26110 (PA34_026110)	pilC	5444153..5445370 (+)	1218	WP_016254080.1	type II secretion system F family protein	Machinery gene
PA34_RS26115 (PA34_026115)	pilD	5445373..5446245 (+)	873	WP_034007824.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
PA34_RS26120 (PA34_026120)	coaE	5446242..5446853 (+)	612	WP_033989050.1	dephospho-CoA kinase	-
PA34_RS26125 (PA34_026125)	yacG	5446850..5447050 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

• Length: 179 a.a.
• Molecular weight: 18083.45 Da
• Isoelectric Point: 5.0804

>NTDB_id=276767 PA34_RS26100 WP_003141352.1 5441678..5442217(-) (pilE) [Pseudomonas aeruginosa strain PA34]
MKAQKGFTLIELMIVVAIIGILAAVALPAYQDYTIRARVTEGVGLAASAKTLIGDSSATAGELAASARVWNAQAGNAGAT
SKYVTSVRIAEATGEITVTFNATNVGNIPADSTLVFTPYVQNAAGAPTQLGASYAAGVTGSIDWGCASESNAVSSGPDRN
MPALTAGTLPARFAPSECR

Nucleotide

• Length: 540 bp

>NTDB_id=276767 PA34_RS26100 WP_003141352.1 5441678..5442217(-) (pilE) [Pseudomonas aeruginosa strain PA34]
ATGAAAGCTCAGAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATTCTTGCTGCCGTCGCTTT
GCCGGCATATCAGGATTACACCATTCGTGCTCGCGTGACAGAGGGGGTTGGTCTGGCTGCCAGCGCCAAGACGCTTATTG
GCGATAGCTCTGCCACTGCCGGTGAGCTAGCCGCTTCGGCAAGGGTCTGGAATGCTCAAGCCGGTAACGCCGGTGCTACC
AGTAAGTATGTGACCTCTGTGCGAATTGCAGAGGCGACTGGTGAAATCACTGTTACTTTCAATGCCACAAACGTGGGTAA
TATTCCGGCTGACTCTACCCTGGTATTTACTCCCTATGTGCAGAATGCTGCCGGTGCCCCGACTCAATTGGGTGCCAGTT
ATGCTGCCGGTGTGACTGGCTCTATTGACTGGGGTTGTGCATCGGAATCCAATGCGGTTTCCAGTGGTCCCGACCGCAAT
ATGCCTGCCCTGACTGCAGGTACCCTGCCGGCTCGTTTCGCTCCGAGCGAATGCCGCTAA

Domains

Predicted by InterProScan.

• Pilin (bacterial filament) (Pilin) (36-179)

• Prokaryotic N-terminal methylation motif (N_methyl) (1-27)

3D structure

• AlphaFold DB: A0A0H2ZFW8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	42.64	100	0.469
pilA	Ralstonia pseudosolanacearum GMI1000	43.243	100	0.447
pilA2	Legionella pneumophila str. Paris	41.808	98.883	0.413
pilA2	Legionella pneumophila strain ERS1305867	41.243	98.883	0.408
comP	Acinetobacter baylyi ADP1	38.333	100	0.385
pilE	Neisseria gonorrhoeae strain FA1090	35.714	100	0.363