Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   SBAL_RS20240 Genome accession   NC_009052
Coordinates   4613428..4615140 (+) Length   570 a.a.
NCBI ID   WP_011848005.1    Uniprot ID   A3D9H6
Organism   Shewanella baltica OS155     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4608428..4620140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SBAL_RS20220 (Sbal_3915) ampE 4609285..4610136 (-) 852 WP_011848001.1 beta-lactamase regulator AmpE -
  SBAL_RS20225 (Sbal_3916) ampD 4610206..4610769 (-) 564 WP_011848002.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  SBAL_RS20230 (Sbal_3917) nadC 4611303..4612184 (+) 882 WP_011848003.1 carboxylating nicotinate-nucleotide diphosphorylase -
  SBAL_RS20235 (Sbal_3918) - 4612907..4613338 (+) 432 WP_011848004.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  SBAL_RS20240 (Sbal_3919) pilB 4613428..4615140 (+) 1713 WP_011848005.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SBAL_RS20245 (Sbal_3920) pilC 4615274..4616542 (+) 1269 WP_011848006.1 type II secretion system F family protein Machinery gene
  SBAL_RS20250 (Sbal_3921) pilD 4616576..4617490 (+) 915 WP_011848007.1 A24 family peptidase Machinery gene
  SBAL_RS20255 (Sbal_3922) coaE 4617530..4618147 (+) 618 WP_011848008.1 dephospho-CoA kinase -
  SBAL_RS20260 (Sbal_3923) zapD 4618140..4618874 (+) 735 WP_011848009.1 cell division protein ZapD -
  SBAL_RS20265 (Sbal_3924) yacG 4618948..4619157 (+) 210 WP_011848010.1 DNA gyrase inhibitor YacG -
  SBAL_RS20270 (Sbal_3925) mutT 4619234..4619626 (+) 393 WP_011848011.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62706.17 Da        Isoelectric Point: 6.2551

>NTDB_id=27603 SBAL_RS20240 WP_011848005.1 4613428..4615140(+) (pilB) [Shewanella baltica OS155]
MPTTGLHLGLSTLFIRKGLLNEEQMANAITKSRQNKHALVSTLVQSKLVSARAIADLCYEEYGTPLLDLNEFDLASIPED
FLNKKLIEKHRCLPLFKRGNRLYIGTSDPTNIAALEDFQFSAGLHAEAILVEEDKLSKALEKILEEDISGLDLGGMDEAA
LAAIEITDTDNRSDVSAGEASDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLAGRLSA
RLKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEPDQEKLYLEMLAKP
QGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCPECKQAEVIPEHELLRLGF
AQAQITQGFTTFKPIGCEHCSAGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIASQAKAQGMRDLRQSGLLKVMHGVTSI
AEVNRVTSFG

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=27603 SBAL_RS20240 WP_011848005.1 4613428..4615140(+) (pilB) [Shewanella baltica OS155]
ATGCCTACAACTGGTCTTCATTTAGGTTTATCCACCTTATTTATCCGTAAAGGTCTGCTAAATGAAGAGCAGATGGCTAA
CGCCATCACTAAATCCCGTCAAAACAAACACGCCCTTGTAAGCACTTTAGTTCAAAGTAAGCTGGTCTCTGCCCGTGCAA
TAGCAGATCTTTGCTACGAAGAATACGGCACGCCACTACTCGATCTCAATGAGTTTGATCTTGCTTCCATTCCTGAGGAT
TTTCTCAATAAAAAGCTGATTGAGAAACACCGCTGCTTACCCTTGTTTAAACGTGGGAATCGCCTCTATATAGGCACATC
CGACCCGACCAATATTGCCGCCCTTGAAGACTTCCAATTTAGTGCAGGCCTGCATGCCGAAGCGATTCTAGTGGAGGAAG
ATAAACTATCTAAAGCGCTGGAAAAAATTCTCGAGGAAGATATCAGTGGCCTAGATTTAGGCGGTATGGATGAAGCGGCG
CTGGCGGCCATAGAAATTACTGACACAGACAATCGCAGCGATGTGTCAGCAGGCGAGGCCAGTGACGACGCGCCTATTGT
TATTTATATAAACAAAATTCTAACCGACGCCATTCGCAAAGGCGCCTCGGATTTACACTTTGAGCCCTATGAAAAGCGCT
ACCGCATTCGTTTTCGCATCGACGGTATTCTCCACGAAGTCTCAGAGCCTCCTATTAGCCTCGCAGGCCGATTATCTGCT
AGGTTAAAGGTAATGTCTAAGCTCGATATCGCCGAACGGCGCGTGCCACAAGATGGTCGCATTAAAATGAAATTGTCCCG
TACTAAGTCCATCGACTTTCGGGTAAGTACCCTACCAACACTGTGGGGGGAAAAAATTGTAATGCGGATTTTGGACTCAT
CCTCCGCACAATTAGGCATTGAAAAGCTCGGCTACGAGCCCGACCAAGAAAAGCTCTACCTTGAAATGCTAGCAAAACCC
CAAGGTATGATTTTAGTGACTGGCCCTACGGGCTCAGGTAAAACCGTCTCCCTTTACACTGGGTTAAATATTCTCAATAC
CGAAGAGCGTAATATTTCCACCGCTGAAGATCCGGTTGAGATTAACCTTGAGGGCGTGAATCAGGTTCATATTAATTTAA
AAGCAGGGCTCACCTTCGCGTCCGCTTTACGTTCATTTCTACGCCAAGACCCCGATGTGGTTATGGTCGGGGAAATCCGT
GATCTTGAAACCGCCGAAATTGCTATTAAAGCGGCGCAAACGGGTCACTTAGTTTTATCAACCTTGCATACTAACTCGGC
AGCCGAAACCTTAACCCGTTTAATCAATATGGGTGTGCCTGGCTACAACATAGCCAGCTCTGTGAATCTGATTATTGCCC
AGCGCTTAGCCCGTCGTCTCTGCCCTGAATGCAAGCAAGCGGAAGTGATCCCCGAGCATGAATTATTGCGTTTAGGCTTT
GCCCAAGCACAAATTACTCAAGGCTTTACCACCTTTAAACCTATTGGCTGCGAGCACTGCTCTGCGGGCTACAAAGGCCG
AGTCGGTATTTATGAAGTGATGAAAATGTCCGATGAAATAGCCCGTACTATCATGGAGGGCGGTAATTCTTTGCAAATTG
CCTCCCAAGCTAAAGCACAAGGAATGCGAGATTTACGGCAATCAGGGTTATTGAAAGTGATGCATGGCGTCACTAGCATT
GCAGAAGTCAATCGCGTCACCAGTTTTGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A3D9H6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.358

98.421

0.584
  pilB Acinetobacter baumannii D1279779

58.899

95.614

0.563
  pilB Legionella pneumophila strain ERS1305867

56.228

98.596

0.554
  pilB Vibrio cholerae strain A1552

53.142

97.719

0.519
  pilB Vibrio parahaemolyticus RIMD 2210633

52.021

99.825

0.519
  pilB Vibrio campbellii strain DS40M4

51.413

99.298

0.511
  pilF Neisseria gonorrhoeae MS11

47.703

99.298

0.474
  pilF Thermus thermophilus HB27

39.046

99.298

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.75

98.246

0.381

Multiple sequence alignment