Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CSB91_RS15390 Genome accession   NZ_CP027171
Coordinates   3266973..3268673 (+) Length   566 a.a.
NCBI ID   WP_058137985.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain AR_0354     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3261973..3273673
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CSB91_RS15370 (CSB91_3085) - 3262686..3264941 (-) 2256 WP_003094643.1 DUF1631 domain-containing protein -
  CSB91_RS15375 (CSB91_3086) nadC 3265227..3266075 (+) 849 WP_003094645.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CSB91_RS15385 - 3266279..3266743 (-) 465 WP_031630343.1 pilin -
  CSB91_RS15390 (CSB91_3088) pilB 3266973..3268673 (+) 1701 WP_058137985.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CSB91_RS15395 (CSB91_3089) pilC 3268677..3269897 (+) 1221 WP_003094649.1 type II secretion system F family protein Machinery gene
  CSB91_RS15400 (CSB91_3090) pilD 3269901..3270773 (+) 873 WP_003094651.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  CSB91_RS15405 (CSB91_3091) coaE 3270770..3271381 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  CSB91_RS15410 (CSB91_3092) yacG 3271378..3271578 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  CSB91_RS15415 (CSB91_3093) - 3271615..3271824 (-) 210 WP_003094660.1 hypothetical protein -
  CSB91_RS15420 (CSB91_3094) - 3271930..3272619 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  CSB91_RS15425 (CSB91_3095) - 3272616..3273086 (-) 471 WP_003094664.1 hypothetical protein -
  CSB91_RS15430 (CSB91_3096) - 3273083..3273508 (-) 426 WP_003094666.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62386.25 Da        Isoelectric Point: 5.5814

>NTDB_id=275400 CSB91_RS15390 WP_058137985.1 3266973..3268673(+) (pilB) [Pseudomonas aeruginosa strain AR_0354]
MNDSTPLSGLAKQLVLNELLDEKTARQAQVQAARNKQSLVSHLVQAKLVQSRALVEVAAEQFGIAYCDLNSLDRESIPKD
AISERLVRQHRVLPLWKRGNKLFIGLSDPTNHQAVTDIQFSTGLTTEALLVEDDKLGVAIEKLYEGAMDALGDLGDIDLD
GVDVGKAESASQEDSGDDAEDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKTYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKNKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQN
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
DKIGTFKLYSPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=275400 CSB91_RS15390 WP_058137985.1 3266973..3268673(+) (pilB) [Pseudomonas aeruginosa strain AR_0354]
ATGAACGATTCCACGCCTCTCAGCGGCCTTGCCAAGCAACTGGTCCTGAACGAACTCCTCGACGAAAAGACCGCCCGCCA
GGCCCAGGTGCAGGCCGCGCGCAACAAGCAGTCGCTGGTCAGCCACCTGGTCCAGGCCAAGCTGGTGCAGAGCCGGGCGT
TGGTCGAGGTGGCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGATCGGGAAAGCATTCCGAAGGAT
GCCATCAGCGAGCGCCTGGTCCGCCAGCACCGCGTGTTGCCGCTGTGGAAGCGCGGCAACAAACTGTTCATCGGCCTCTC
CGACCCGACCAATCACCAAGCGGTCACCGATATCCAGTTCAGCACCGGTCTCACCACCGAAGCCCTGCTGGTGGAGGACG
ACAAGCTCGGCGTAGCCATCGAGAAGCTCTACGAGGGCGCCATGGACGCTCTCGGCGACCTCGGCGATATCGATCTCGAC
GGGGTCGATGTGGGCAAGGCAGAAAGCGCCAGCCAGGAAGATTCCGGCGACGACGCCGAAGATGCCCCGGTGGTGCGCTT
CGTCAACAAGATGCTGCTCGACGCCATCCGCGGCGGCTCTTCCGACCTGCACTTCGAACCCTACGAAAAGACCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAGGTAGCCAAGCCGCCGATCCAGCTGGCCAGCCGCATTTCCGCGCGACTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGGCGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCAAAGAACAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCCCTCGGCTACGAAGAGGATCAGAAGGAACTTTATCTGGCCGCGCTCAAACAGCCACAGAAC
ATGATTCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTTTCGCTGTACACCGGCCTGAACATCCTGAATACCACCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTG
GAGACTGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGCCTGCTGAACATGGGCGTGCCGGCATTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGACGTGCCGAAGGAAACCCTGCTTCACGAGGGCTTCCCGGAA
GACAAGATCGGCACCTTCAAGCTGTATTCGCCTGTAGGCTGCGAAAACTGCAAAGGCGGTTACAAAGGTCGCGTCGGTAT
TTATGAAGTGGTTAAAAACACCCCCGCCCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGAATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.544

100

0.58
  pilB Acinetobacter baylyi ADP1

56.437

100

0.565
  pilB Legionella pneumophila strain ERS1305867

54.466

100

0.549
  pilB Vibrio cholerae strain A1552

51.264

97.88

0.502
  pilF Neisseria gonorrhoeae MS11

50

99.647

0.498
  pilB Vibrio parahaemolyticus RIMD 2210633

52.434

94.346

0.495
  pilB Vibrio campbellii strain DS40M4

51.22

94.17

0.482
  pilB/pilB1 Synechocystis sp. PCC 6803

37.601

100

0.41
  pilF Thermus thermophilus HB27

38.664

100

0.389
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.909

97.173

0.378

Multiple sequence alignment