Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VEIS_RS18965 Genome accession   NC_008786
Coordinates   4306503..4308239 (+) Length   578 a.a.
NCBI ID   WP_041950195.1    Uniprot ID   -
Organism   Verminephrobacter eiseniae EF01-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4301503..4313239
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VEIS_RS18935 (Veis_3914) proB 4302073..4303215 (-) 1143 WP_011811608.1 glutamate 5-kinase -
  VEIS_RS18940 (Veis_3915) obgE 4303348..4304439 (-) 1092 WP_011811609.1 GTPase ObgE -
  VEIS_RS18945 (Veis_3916) rpmA 4304508..4304783 (-) 276 WP_011811610.1 50S ribosomal protein L27 -
  VEIS_RS18950 (Veis_3917) rplU 4304802..4305113 (-) 312 WP_011811611.1 50S ribosomal protein L21 -
  VEIS_RS18955 (Veis_3918) - 4305310..4306239 (+) 930 WP_041951049.1 polyprenyl synthetase family protein -
  VEIS_RS18965 (Veis_3919) pilB 4306503..4308239 (+) 1737 WP_041950195.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VEIS_RS18970 (Veis_3920) pilC 4308265..4309488 (+) 1224 WP_011811614.1 type II secretion system F family protein Machinery gene
  VEIS_RS18975 (Veis_3921) - 4309488..4310372 (+) 885 WP_011811615.1 A24 family peptidase -
  VEIS_RS18980 (Veis_3922) coaE 4310376..4311008 (+) 633 WP_011811616.1 dephospho-CoA kinase -
  VEIS_RS18985 (Veis_3923) zapD 4311052..4311807 (+) 756 WP_011811617.1 cell division protein ZapD -
  VEIS_RS18990 (Veis_3924) - 4311828..4312049 (+) 222 WP_011811618.1 DNA gyrase inhibitor YacG -
  VEIS_RS18995 (Veis_3925) - 4312083..4312550 (-) 468 WP_011811619.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62867.24 Da        Isoelectric Point: 5.0740

>NTDB_id=27318 VEIS_RS18965 WP_041950195.1 4306503..4308239(+) (pilB) [Verminephrobacter eiseniae EF01-2]
MAAVDTPSKEASAVALPGLGRALMSAGKLSQKSAEDIYKKSQANRTSFIAELTGSGAVSAADLAHTVSTVFGAPLLDLDA
IDPSRLPKDLLDNKICKTCRVVVLSKRSNRLIVATADPTDQEAAEKIKFATQMGVDWVIAEYDKLSLLVEAITKSANEAM
DTLVSGGDFEFDDMAVETATEDAAVAATDVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIASPP
IAIKEKLASRIKVISRLDISEKRIPQDGRMKLKVGQNRVIDFRVSTLPTLFGEKVVIRILDPSSAKLGIDALGYEPVEKE
RLLQAIGRPYGMILVTGPTGSGKTVSLYTCLNLLNKPDVNIATAEDPCEINLPGVNQVNVNEKAGLTFAAALRSFLRQDP
DIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPICKTPTDIP
QETLVDAGYQEEEIDGSWVTYGPVGCNACNNGYKGRVGIYQVMPITEEIQRIILRDGSALEIAEQARMEGVRSLRDAGLY
KAKLGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=27318 VEIS_RS18965 WP_041950195.1 4306503..4308239(+) (pilB) [Verminephrobacter eiseniae EF01-2]
ATGGCCGCTGTCGATACTCCCTCCAAAGAAGCCTCTGCCGTTGCACTTCCCGGACTGGGCAGGGCGTTGATGTCCGCAGG
CAAGCTGAGTCAGAAATCGGCCGAGGACATTTACAAGAAGTCCCAGGCCAACCGCACCAGTTTCATTGCCGAATTGACCG
GCTCCGGGGCTGTCTCCGCCGCTGACTTGGCGCATACGGTGTCGACCGTCTTTGGCGCGCCCCTTCTCGATCTCGATGCC
ATCGATCCGTCGCGTTTGCCAAAGGATTTGCTGGATAACAAGATATGCAAGACCTGCCGCGTCGTCGTACTGAGCAAACG
CAGTAATCGCCTGATCGTCGCAACGGCAGACCCTACCGACCAGGAAGCGGCTGAAAAAATCAAGTTTGCCACCCAGATGG
GCGTGGACTGGGTCATCGCGGAATATGACAAACTCAGCCTTCTGGTCGAAGCGATCACCAAATCCGCCAACGAAGCAATG
GATACGCTGGTCAGTGGCGGTGACTTCGAGTTCGACGATATGGCGGTCGAAACTGCCACCGAAGATGCGGCTGTGGCAGC
CACGGATGTCGAAGATGCTCCGATCGTCAAGTTTCTGCACAAAATGCTGCTGGACGCCTTCAACATGCGCGCGTCCGACC
TGCATTTCGAACCCTACGAGCACCAGTACCGCGTCCGCTTCCGCATCGATGGCGAGTTGCGGGAAATAGCTTCGCCGCCG
ATTGCGATCAAGGAAAAGCTGGCTTCCCGCATCAAGGTCATTTCGCGCCTGGATATCTCGGAAAAACGCATTCCACAAGA
TGGCCGCATGAAGCTGAAAGTCGGCCAGAACCGGGTGATCGACTTCCGGGTCAGCACCTTGCCCACCTTGTTTGGCGAGA
AAGTCGTCATCCGTATTCTGGATCCGAGCAGCGCCAAACTGGGTATCGATGCGCTGGGCTATGAACCGGTGGAGAAAGAG
CGGCTACTCCAGGCCATCGGTCGCCCCTACGGCATGATTTTGGTCACAGGCCCTACCGGTTCGGGCAAGACCGTGTCACT
GTATACCTGTCTGAATCTCCTGAACAAACCGGACGTCAATATCGCGACGGCAGAAGACCCTTGCGAAATCAACCTGCCGG
GCGTGAATCAGGTAAACGTCAACGAAAAGGCCGGCCTGACATTTGCTGCGGCGCTGCGCTCCTTCCTGCGCCAGGATCCC
GACATCATCATGGTCGGGGAAATACGGGATCTGGAAACTGCCGACATCTCGATCAAGGCGGCACAAACCGGGCACCTGGT
GCTGTCCACCCTGCACACGAATGACGCGCCGACGACCTTGACACGGATGCGCAACATGGGTATTGCGCCATTCAATATTG
CGTCCAGCGTGATTCTGATCACCGCGCAGCGCCTGGCGCGCAGGCTGTGCCCGATCTGCAAAACCCCGACGGACATACCC
CAGGAGACCCTGGTCGATGCGGGATACCAAGAGGAGGAGATCGACGGCTCCTGGGTTACTTATGGTCCGGTCGGCTGCAA
CGCTTGCAACAATGGCTACAAGGGCCGGGTCGGCATTTATCAGGTGATGCCGATCACAGAGGAAATCCAGCGCATCATCC
TGCGCGATGGCAGCGCCCTGGAGATTGCCGAGCAAGCCCGCATGGAGGGTGTGCGCTCATTGCGCGACGCGGGCCTGTAC
AAGGCCAAGTTGGGCCTGACTTCCCTCGAAGAAGTCCTGGCCGTCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-152)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.276

100

0.535
  pilB Acinetobacter baumannii D1279779

52.921

100

0.533
  pilF Neisseria gonorrhoeae MS11

52.482

97.578

0.512
  pilB Legionella pneumophila strain ERS1305867

50

97.578

0.488
  pilB Vibrio campbellii strain DS40M4

45.922

97.578

0.448
  pilB Vibrio parahaemolyticus RIMD 2210633

45.167

98.443

0.445
  pilB Vibrio cholerae strain A1552

45.455

97.059

0.441
  pilF Thermus thermophilus HB27

40.283

97.924

0.394
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.33

98.097

0.386

Multiple sequence alignment