Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   C5F71_RS14965 Genome accession   NZ_CP026881
Coordinates   3161698..3163029 (+) Length   443 a.a.
NCBI ID   WP_104911418.1    Uniprot ID   -
Organism   Pseudomonas sp. LG1D9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3156698..3168029
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C5F71_RS14945 pilM 3159295..3159948 (+) 654 WP_104911416.1 type IV pilus biogenesis protein PilM -
  C5F71_RS14950 - 3159948..3160499 (+) 552 WP_029296063.1 PilN domain-containing protein -
  C5F71_RS14955 - 3160496..3161116 (+) 621 WP_104911417.1 type 4a pilus biogenesis protein PilO -
  C5F71_RS29710 - 3161113..3161628 (+) 516 WP_371923346.1 pilus assembly protein PilP -
  C5F71_RS14965 pilQ 3161698..3163029 (+) 1332 WP_104911418.1 type IV pilus secretin PilQ Machinery gene
  C5F71_RS14970 aroK 3163034..3163552 (+) 519 WP_029296072.1 shikimate kinase AroK -
  C5F71_RS14975 aroB 3163700..3164800 (+) 1101 WP_057958082.1 3-dehydroquinate synthase -
  C5F71_RS14980 - 3164811..3166385 (+) 1575 WP_104911419.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 443 a.a.        Molecular weight: 48020.94 Da        Isoelectric Point: 4.8396

>NTDB_id=273067 C5F71_RS14965 WP_104911418.1 3161698..3163029(+) (pilQ) [Pseudomonas sp. LG1D9]
MTEAVPKRIDLIQLPPPGASPLGAGRGAYSGDRLSLNFQDIEVRAVLQQIADVAGLNLVAGDDVQGSITLRLKDVPWDQA
LDLVLQAKGLDKRVTANVLLVAPAEELAARELLALESRKLMTELAPLRRELLQVNYAKAAELAKLFQSVSGPEGVADERG
SVTVDERTNNIIAYQTGERLEELRRIVNQLDIPVRQVMIEARIVEANVDYDKSVGVRWGGLLRNKGHWSGGGVGKSPSGE
GEPPDSSPFVDLGVVNASSGLGIAFITDNVLLDLELTAMEKGGNGEVISQPKVVTSNKETARILKGTEIPYQEATAEGAT
SVSFKEASLSLEVTPQITPDDQVIMEVRVTKDEPDYLNKLNDVPPIKKNEVTAKVLVRDGETIVLGGVFSKNQSKVVDKV
PFLGDVPYLGRLFRRDVVAQRKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1332 bp        

>NTDB_id=273067 C5F71_RS14965 WP_104911418.1 3161698..3163029(+) (pilQ) [Pseudomonas sp. LG1D9]
ATGACTGAGGCTGTGCCCAAGCGGATTGACCTGATTCAGTTGCCACCCCCTGGTGCTTCGCCTTTGGGCGCCGGACGAGG
GGCCTATAGCGGTGACAGGCTCTCTCTCAACTTCCAGGACATCGAGGTCCGCGCAGTCCTGCAGCAGATTGCCGACGTGG
CTGGCCTCAACCTGGTGGCCGGTGATGATGTGCAGGGCTCGATCACCCTGCGCCTCAAGGACGTGCCCTGGGATCAGGCC
CTGGACCTGGTGCTGCAAGCCAAGGGGCTCGACAAGCGGGTGACGGCCAATGTGCTGCTGGTGGCGCCGGCTGAGGAACT
GGCCGCCCGTGAGCTGCTGGCCTTGGAGTCGCGCAAGCTGATGACCGAGCTGGCGCCCTTGCGGCGGGAGCTGCTGCAAG
TCAATTACGCCAAGGCGGCCGAGTTGGCCAAGCTGTTCCAGTCGGTGAGCGGGCCTGAGGGCGTGGCCGATGAGCGTGGT
TCGGTGACGGTAGATGAGCGTACCAACAACATCATCGCCTACCAGACGGGGGAGCGCCTGGAGGAGCTGCGGCGCATCGT
CAATCAATTGGATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGGATAGTCGAGGCCAATGTCGACTATGACAAAAGCG
TGGGCGTGCGCTGGGGTGGGTTGTTGCGCAATAAAGGTCATTGGAGCGGGGGTGGGGTTGGTAAAAGCCCGTCCGGCGAG
GGCGAGCCGCCCGATAGCTCGCCGTTCGTTGACCTGGGCGTGGTCAACGCAAGCTCTGGTCTGGGCATCGCGTTTATCAC
CGATAACGTGCTGCTGGACCTGGAACTCACAGCCATGGAAAAAGGCGGCAATGGCGAGGTGATCTCCCAGCCCAAGGTGG
TCACTTCCAACAAGGAGACCGCACGGATTCTCAAGGGCACCGAGATCCCCTACCAGGAAGCCACTGCCGAGGGTGCGACG
TCGGTGTCGTTCAAGGAGGCCTCGCTTTCTTTGGAGGTGACGCCGCAAATCACCCCGGATGACCAGGTCATCATGGAGGT
CCGGGTGACCAAGGATGAGCCGGACTACCTGAACAAACTCAACGATGTGCCGCCGATCAAGAAGAACGAGGTCACTGCCA
AGGTTCTGGTCAGGGACGGCGAGACCATCGTCCTGGGCGGGGTTTTCTCCAAAAACCAAAGCAAGGTGGTAGATAAAGTG
CCATTTTTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTCGTTGCCCAAAGAAAATCCGAGCTGCTGGT
ATTCCTGACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(278-434)

Bacterial type II/III secretion system short domain (Secretin_N)

(131-196)

Secretin and TonB N terminus short domain (STN)

(57-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

69.635

98.871

0.688
  comQ Acinetobacter baylyi ADP1

43.556

100

0.442
  pilQ Acinetobacter baumannii strain A118

41.81

100

0.438
  pilQ Acinetobacter baumannii D1279779

41.81

100

0.438
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

45.455

94.357

0.429
  pilQ Vibrio cholerae strain A1552

45.455

94.357

0.429
  pilQ Vibrio campbellii strain DS40M4

44.917

95.485

0.429
  pilQ Legionella pneumophila strain ERS1305867

44.681

95.485

0.427
  pilQ Legionella pneumophila strain Lp02

44.681

95.485

0.427

Multiple sequence alignment