Detailed information NTG0271869

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: AM435_RS12030
• Genome accession: NZ_CP026707
• Coordinates: 2223912..2224970 (-)
• Length: 352 a.a.
• NCBI ID: WP_004644147.1
• Uniprot ID: A0A7U3Y305
• Organism: Acinetobacter baumannii strain AR_0056
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2218912..2229970

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AM435_RS12005 (AM435_12000)	aroK	2219187..2219756 (-)	570	WP_000643712.1	shikimate kinase AroK	-
AM435_RS12010 (AM435_12005)	pilQ	2219768..2221933 (-)	2166	WP_001017033.1	type IV pilus secretin PilQ family protein	Machinery gene
AM435_RS12015 (AM435_12010)	pilP	2221996..2222523 (-)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
AM435_RS12020 (AM435_12015)	pilO	2222534..2223274 (-)	741	WP_000076099.1	type 4a pilus biogenesis protein PilO	Machinery gene
AM435_RS12025 (AM435_12020)	pilN	2223271..2223912 (-)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
AM435_RS12030 (AM435_12025)	pilM	2223912..2224970 (-)	1059	WP_004644147.1	pilus assembly protein PilM	Machinery gene
AM435_RS12035 (AM435_12030)	ponA	2225132..2227687 (+)	2556	WP_000736667.1	penicillin-binding protein PBP1a	-
AM435_RS12040 (AM435_12035)	-	2227705..2228529 (-)	825	WP_002022249.1	putative RNA methyltransferase	-
AM435_RS12045 (AM435_12040)	-	2228532..2228864 (-)	333	WP_000169602.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39062.61 Da
• Isoelectric Point: 4.4618

>NTDB_id=271869 AM435_RS12030 WP_004644147.1 2223912..2224970(-) (pilM) [Acinetobacter baumannii strain AR_0056]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLTNPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=271869 AM435_RS12030 WP_004644147.1 2223912..2224970(-) (pilM) [Acinetobacter baumannii strain AR_0056]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCGGATCGTCTTA
CGAATCCAAATCGTGTAAATGTGCTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGCCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTTTTCGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGCAGAGCGAAAAAAGATCGTTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGCTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTCGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: A0A7U3Y305

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	99.716	100	0.997
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.25	100	0.813
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423