Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   AM435_RS12025 Genome accession   NZ_CP026707
Coordinates   2223271..2223912 (-) Length   213 a.a.
NCBI ID   WP_000201227.1    Uniprot ID   A0A009HSE6
Organism   Acinetobacter baumannii strain AR_0056     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2218271..2228912
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AM435_RS12005 (AM435_12000) aroK 2219187..2219756 (-) 570 WP_000643712.1 shikimate kinase AroK -
  AM435_RS12010 (AM435_12005) pilQ 2219768..2221933 (-) 2166 WP_001017033.1 type IV pilus secretin PilQ family protein Machinery gene
  AM435_RS12015 (AM435_12010) pilP 2221996..2222523 (-) 528 WP_000695065.1 pilus assembly protein PilP Machinery gene
  AM435_RS12020 (AM435_12015) pilO 2222534..2223274 (-) 741 WP_000076099.1 type 4a pilus biogenesis protein PilO Machinery gene
  AM435_RS12025 (AM435_12020) pilN 2223271..2223912 (-) 642 WP_000201227.1 PilN domain-containing protein Machinery gene
  AM435_RS12030 (AM435_12025) pilM 2223912..2224970 (-) 1059 WP_004644147.1 pilus assembly protein PilM Machinery gene
  AM435_RS12035 (AM435_12030) ponA 2225132..2227687 (+) 2556 WP_000736667.1 penicillin-binding protein PBP1a -
  AM435_RS12040 (AM435_12035) - 2227705..2228529 (-) 825 WP_002022249.1 putative RNA methyltransferase -
  AM435_RS12045 (AM435_12040) - 2228532..2228864 (-) 333 WP_000169602.1 hypothetical protein -

Sequence

Protein


Download         Length: 213 a.a.        Molecular weight: 24019.35 Da        Isoelectric Point: 5.3968

>NTDB_id=271868 AM435_RS12025 WP_000201227.1 2223271..2223912(-) (pilN) [Acinetobacter baumannii strain AR_0056]
MATINLLPWREELREQRKKQFITLCFGVVVLGITTVFGGWFYFDHKLDDQEQANQLIMSTNQNLDQQLKTLNGLQEQRDA
IIERMKLIQGLQSQRPVVVRLVDELVRVTPSAMYLTKFSRTGDKFTIEGKAESPNTVAELLRNLEASPWYRNAFMNSFLA
NEEKKDKAASSLLPRVEDHYGSFVVTVDLGEMGVTTTDDSAKPSTGESVGAAK

Nucleotide


Download         Length: 642 bp        

>NTDB_id=271868 AM435_RS12025 WP_000201227.1 2223271..2223912(-) (pilN) [Acinetobacter baumannii strain AR_0056]
ATGGCAACAATTAATTTACTGCCTTGGCGCGAAGAGCTGAGGGAGCAACGAAAAAAACAATTTATAACGTTATGTTTTGG
GGTGGTTGTTTTGGGGATTACAACCGTATTTGGGGGATGGTTTTATTTTGACCATAAATTAGATGATCAAGAGCAAGCGA
ATCAGCTCATTATGAGCACAAATCAGAACCTTGATCAGCAATTAAAAACATTAAACGGCTTACAGGAACAGCGTGATGCC
ATTATTGAACGCATGAAGTTGATTCAAGGTTTGCAGAGTCAACGTCCAGTAGTGGTAAGACTTGTTGATGAGTTAGTTCG
AGTCACACCATCAGCTATGTATTTAACGAAATTTAGCCGAACCGGAGATAAGTTTACGATTGAAGGTAAAGCTGAAAGTC
CAAATACAGTAGCCGAACTACTCCGTAATCTTGAAGCATCTCCTTGGTATCGTAATGCTTTTATGAACTCTTTCTTGGCT
AATGAAGAGAAAAAAGATAAGGCTGCAAGTTCTCTTCTCCCTAGGGTGGAAGACCACTATGGTAGTTTTGTTGTGACTGT
CGATTTGGGGGAAATGGGCGTGACCACAACGGATGATTCTGCTAAGCCATCTACAGGTGAAAGTGTGGGGGCTGCTAAAT
GA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(100-167)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A009HSE6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Acinetobacter baumannii D1279779

100

100

1
  comN Acinetobacter baylyi ADP1

71.905

98.592

0.709

Multiple sequence alignment