Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   AM435_RS12020 Genome accession   NZ_CP026707
Coordinates   2222534..2223274 (-) Length   246 a.a.
NCBI ID   WP_000076099.1    Uniprot ID   A0A9P2QDS6
Organism   Acinetobacter baumannii strain AR_0056     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2217534..2228274
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AM435_RS12000 (AM435_11995) aroB 2218076..2219158 (-) 1083 WP_001193991.1 3-dehydroquinate synthase -
  AM435_RS12005 (AM435_12000) aroK 2219187..2219756 (-) 570 WP_000643712.1 shikimate kinase AroK -
  AM435_RS12010 (AM435_12005) pilQ 2219768..2221933 (-) 2166 WP_001017033.1 type IV pilus secretin PilQ family protein Machinery gene
  AM435_RS12015 (AM435_12010) pilP 2221996..2222523 (-) 528 WP_000695065.1 pilus assembly protein PilP Machinery gene
  AM435_RS12020 (AM435_12015) pilO 2222534..2223274 (-) 741 WP_000076099.1 type 4a pilus biogenesis protein PilO Machinery gene
  AM435_RS12025 (AM435_12020) pilN 2223271..2223912 (-) 642 WP_000201227.1 PilN domain-containing protein Machinery gene
  AM435_RS12030 (AM435_12025) pilM 2223912..2224970 (-) 1059 WP_004644147.1 pilus assembly protein PilM Machinery gene
  AM435_RS12035 (AM435_12030) ponA 2225132..2227687 (+) 2556 WP_000736667.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27801.55 Da        Isoelectric Point: 4.8357

>NTDB_id=271867 AM435_RS12020 WP_000076099.1 2222534..2223274(-) (pilO) [Acinetobacter baumannii strain AR_0056]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTIHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGATDTSEQTGKAPAASASTN
TTVQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=271867 AM435_RS12020 WP_000076099.1 2222534..2223274(-) (pilO) [Acinetobacter baumannii strain AR_0056]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCGAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTGCAACTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTACCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTGGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACCGGGGACTATCACGCATTTGGAGCTTTTGTAAGTAGTATCGCTGCTTTGCCTCGTATTGTAAC
CATTCACGATTTTACTGTTGATGTATCGCCAGCTAAAGATAACAAGTCTGATATTCCTGTGCTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGGCAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCTTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

99.593

100

0.996
  comO Acinetobacter baylyi ADP1

69.421

98.374

0.683

Multiple sequence alignment