Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C2U37_RS10025 Genome accession   NZ_CP026228
Coordinates   2062110..2063816 (-) Length   568 a.a.
NCBI ID   WP_104452202.1    Uniprot ID   -
Organism   Aeromonas sp. ASNIH1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2057110..2068816
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2U37_RS09995 (C2U37_09990) mutT 2057728..2058132 (+) 405 WP_174213873.1 8-oxo-dGTP diphosphatase MutT -
  C2U37_RS10000 (C2U37_09995) yacG 2058190..2058384 (-) 195 WP_039040499.1 DNA gyrase inhibitor YacG -
  C2U37_RS10005 (C2U37_10000) zapD 2058395..2059117 (-) 723 WP_010675825.1 cell division protein ZapD -
  C2U37_RS10010 (C2U37_10005) coaE 2059155..2059769 (-) 615 WP_104452200.1 dephospho-CoA kinase -
  C2U37_RS10015 (C2U37_10010) pilD 2059788..2060663 (-) 876 WP_104452201.1 A24 family peptidase Machinery gene
  C2U37_RS10020 (C2U37_10015) pilC 2060741..2061982 (-) 1242 WP_010675822.1 type II secretion system F family protein Machinery gene
  C2U37_RS10025 (C2U37_10020) pilB 2062110..2063816 (-) 1707 WP_104452202.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  C2U37_RS10030 (C2U37_10025) - 2063820..2064260 (-) 441 WP_104452203.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  C2U37_RS10035 (C2U37_10030) nadC 2064571..2065434 (-) 864 WP_041211785.1 carboxylating nicotinate-nucleotide diphosphorylase -
  C2U37_RS10040 (C2U37_10035) - 2065438..2065908 (-) 471 WP_104452204.1 retropepsin-like aspartic protease -
  C2U37_RS10045 (C2U37_10040) ampD 2066037..2066609 (+) 573 WP_102949050.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  C2U37_RS10050 (C2U37_10045) pdhR 2066886..2067650 (+) 765 WP_010675815.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62621.98 Da        Isoelectric Point: 5.8214

>NTDB_id=267948 C2U37_RS10025 WP_104452202.1 2062110..2063816(-) (pilB) [Aeromonas sp. ASNIH1]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQARAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTAAIGKLMESEQDTLGMDHIDEAEI
SELEVSDENSRLDESVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRVDGILHEVATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIDQLGFDDRQKTQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=267948 C2U37_RS10025 WP_104452202.1 2062110..2063816(-) (pilB) [Aeromonas sp. ASNIH1]
ATGACCTCTAGCCTTAATAGCGGCCTGGCCACCAGCCTGGCCGCTTCCTCCCTCCTCAGTGAGCCGGACTCACAGCGCTA
TCTGAGCCAGGCCAGGGCGCAGCGCAAGCCCTTTGTCACCTTCCTGATTGAAAACGACATCCTCGACAGCAAGGCCCTCG
CCGACTTTTGCGAGCTTGAGTATGGGGTACCTCTGCTGGATCTGGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAAAAGCTGATTGAAAAACACCACGTCCTGCCCATCTACACCCAGGGGCATACCCTCTATATCGCCATGTCCGA
TCCGACCAACGTGTCGGCCCTGGAGGATTTCGGCTTCAGCTTCGGTCTGCACACGGAAGCCCTGCTGGTCGAGGAGAGCA
AGCTGACCGCCGCCATCGGCAAGTTGATGGAGAGCGAGCAGGATACCCTCGGCATGGATCACATCGACGAGGCCGAGATC
TCCGAGCTCGAGGTCTCCGACGAGAATTCCCGCCTCGATGAGAGCGTCAACACCGCGGACGATGACGCCCCCATCGTCAA
ATACATCCACAAGATCATGATGGATGCCATCAAGCGCGGGGCCTCCGACCTGCATTTCGAACCCTACGAGACCAAGTACC
GGATCCGCTTTCGGGTAGACGGCATACTGCACGAAGTCGCCACGCCGCCGGTCAACCTGGCCAACCGCTTCGCGGCTCGC
CTCAAGGTGATGGCGCGGCTCGACATTGCCGAACGGCGCCTGCCCCAGGATGGCCGGATCAAACTCAAGATCTCACGCAA
CAGATCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAAAAGATCGTGATCCGGTTGCTGGACTCCTCGG
CCGCCCGGCTCAATATCGATCAACTCGGCTTTGACGACCGGCAGAAGACGCAATACCTGCACGCCCTCTCCAAACCTCAG
GGCATGATCCTGGTGACCGGCCCGACGGGGTCAGGCAAGACGGTCTCACTCTATACCGGCCTCAACATCCTCAACACCAG
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTCGAGATAAACCTGCCCGGCGTCAACCAGGTGCAGATCAATCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGACATCGTCATGGTGGGGGAGATACGGGAT
TTGGAAACCGCCGAGATTGCTATCAAGGCCGCCCAGACCGGTCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCGGC
AGAAACGCTCACCCGGATGATGAACATGGGGGTGCCGGCCTTCAACATCGCCTCTTCGGTCACCCTCATCATGGCCCAGC
GCCTTGCCCGCAAACTCTGCGATCACTGCAAGGCGCCGGAAGTGGTGCCGGAAGCCGAGTTGCTGGAGCTGGGCTTCACC
CAGCAGCAACTGGCCGCCGGGTTGCGGCTGTTCAAGCCCGTGGGATGCAAGGAGTGCTCGGGAGGCTACAGAGGCCGGGT
CGGCATCTATGAGATCATGCTGATGTCCGACAACATCGCCAAACTCATCATGCAGGGAGCCAACTCGCTGCAGATTGCCG
CGATAGCCCAAAAGGAAGGGATGCGCACCCTGCGCACATCCGGCCTCGAGAAAGCGCGTCTTGGCGTCACCAGTCTGGCC
GAAATCAACAGGATCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.85

99.824

0.548
  pilB Legionella pneumophila strain ERS1305867

54.867

99.472

0.546
  pilB Acinetobacter baylyi ADP1

54.885

99.12

0.544
  pilB Vibrio cholerae strain A1552

53.415

100

0.537
  pilB Vibrio campbellii strain DS40M4

50.353

99.648

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

49.911

99.12

0.495
  pilF Neisseria gonorrhoeae MS11

46.561

99.824

0.465
  pilF Thermus thermophilus HB27

40.152

92.958

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

37.975

97.359

0.37

Multiple sequence alignment