Detailed information NTG0267884

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: C2U39_RS02705
• Genome accession: NZ_CP026222
• Coordinates: 524938..526653 (-)
• Length: 571 a.a.
• NCBI ID: WP_176722689.1
• Uniprot ID: -
• Organism: Aeromonas sp. ASNIH3
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	527074..528021	524938..526653	flank	421

Gene organization within MGE regions

Location: 524938..528021

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C2U39_RS02705 (C2U39_02705)	pilB	524938..526653 (-)	1716	WP_176722689.1	PilB family type IVa pilus assembly ATPase TapB	Machinery gene
C2U39_RS24770 (C2U39_02710)	-	526644..527069 (-)	426	WP_324826121.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
C2U39_RS02715 (C2U39_02715)	-	527074..528021 (-)	948	WP_019706001.1	IS30-like element ISAs2 family transposase	-

Sequence

Protein

• Length: 571 a.a.
• Molecular weight: 62974.47 Da
• Isoelectric Point: 5.8214

>NTDB_id=267884 C2U39_RS02705 WP_176722689.1 524938..526653(-) (pilB) [Aeromonas sp. ASNIH3]
MYLMTSSLNSGLATSLAASSLLSEPDSQRYLSQAKAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIP
QKYLNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTAAIGKLMESEQDTLGMDHIDE
AEISELEVSDESSRLDESVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRVDGILHEVATPPVNLANRF
AARLKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIDQLGFDDRQKTQYLHALS
KPQGMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGE
IRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLEL
GFTQQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVT
SLAEINRITTN

Nucleotide

• Length: 1716 bp

>NTDB_id=267884 C2U39_RS02705 WP_176722689.1 524938..526653(-) (pilB) [Aeromonas sp. ASNIH3]
CTGTATTTAATGACCTCTAGCCTCAATAGCGGCCTGGCCACCAGCCTGGCCGCTTCTTCCCTCCTCAGTGAGCCGGACTC
ACAGCGCTATCTGAGCCAGGCCAAGGCGCAGCGCAAGCCCTTTGTCACCTTCCTGATTGAAAACGACATCCTCGACAGCA
AAGCCCTCGCCGATTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTGGCGGCCTTTGATCTCGCCGAGATCCCG
CAGAAATACCTCAATCAAAAGCTGATTGAAAAGCACCATGTCCTGCCCATCTACACCCAGGGGCATACTCTCTATATCGC
CATGTCCGATCCGACCAATGTGTCGGCTCTGGAGGACTTCGGCTTCAGCTTCGGCCTGCATACGGAAGCCCTGCTGGTCG
AAGAGAGCAAGCTGACCGCCGCCATCGGCAAGCTGATGGAGAGCGAGCAGGATACCCTGGGCATGGATCATATCGACGAG
GCCGAGATCTCCGAACTCGAGGTCTCCGACGAGAGTTCCCGCCTCGACGAGAGCGTCAACACCGCGGACGATGACGCCCC
CATCGTCAAATACATCCACAAGATCATGATGGATGCCATCAAGCGCGGGGCCTCCGACCTGCATTTCGAACCCTACGAGA
CCAAGTACCGAATCCGCTTTCGGGTAGACGGCATACTGCACGAAGTCGCCACGCCGCCGGTCAACCTGGCCAACCGCTTC
GCGGCTCGCCTCAAGGTGATGGCGCGGCTCGACATTGCCGAACGGCGCCTGCCCCAGGATGGCCGCATCAAGCTCAAGAT
CTCACGCAACAGATCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAAAAGATCGTGATCCGGTTGCTGG
ACTCCTCGGCCGCCCGACTCAATATCGATCAGCTCGGCTTTGACGACCGGCAGAAGACGCAATACCTGCACGCCCTCTCC
AAACCTCAGGGCATGATCCTGGTCACCGGTCCCACGGGGTCTGGCAAGACGGTCTCACTCTATACCGGCCTCAACATTCT
CAACACCAGCGAGGTCAATATCTCCACCGCCGAAGATCCGGTTGAGATAAACCTGCCCGGCGTCAACCAGGTGCAGATCA
ATCCCAAAGCAGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGACATCGTCATGGTGGGGGAG
ATACGGGATCTGGAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGTCACCTGGTGCTCTCCACCCTGCACACCAA
CTCGGCGGCAGAAACGCTCACCCGGATGATGAACATGGGGGTGCCGGCCTTCAACATCGCCTCTTCGGTCACCCTCATCA
TGGCCCAGCGCCTTGCCCGCAAACTCTGCGATCACTGCAAGGCGCCGGAAGTGGTGCCGGAAGCCGAGTTGCTGGAGCTG
GGCTTCACCCAGCAGCAACTGGCCGCCGGGTTGCGGCTGTTCAAGCCCGTGGGATGCAAGGAGTGCTCGGGAGGCTACAG
AGGCCGGGTCGGCATCTATGAGATCATGCTGATGTCCGACAACATCGCCAAACTCATCATGCAGGGAGCCAACTCGCTGC
AGATTGCCGCGATAGCCCAGAAGGAAGGGATGCGCACCCTGCGTACATCCGGCCTCGAGAAAGCGCGTCTTGGCGTCACC
AGCCTGGCCGAAATCAACAGGATCACCACCAACTGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (45-150)

• Type II/IV secretion system protein (T2SSE) (194-461)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	55.009	99.65	0.548
pilB	Acinetobacter baylyi ADP1	55.062	98.599	0.543
pilB	Legionella pneumophila strain ERS1305867	54.867	98.949	0.543
pilB	Vibrio cholerae strain A1552	53.415	100	0.534
pilB	Vibrio campbellii strain DS40M4	50.53	99.124	0.501
pilB	Vibrio parahaemolyticus RIMD 2210633	50.089	98.599	0.494
pilF	Neisseria gonorrhoeae MS11	46.561	99.299	0.462
pilF	Thermus thermophilus HB27	40.152	92.469	0.371
pilB/pilB1	Synechocystis sp. PCC 6803	37.975	96.848	0.368