Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbB   Type   Machinery gene
Locus tag   C0971_RS17135 Genome accession   NZ_CP026143
Coordinates   3363665..3364042 (-) Length   125 a.a.
NCBI ID   WP_003346745.1    Uniprot ID   I3EBG5
Organism   Bacillus methanolicus strain DFS2     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 3331870..3371630 3363665..3364042 within 0


Gene organization within MGE regions


Location: 3331870..3371630
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C0971_RS16985 (C0971_17010) - 3331870..3332274 (-) 405 WP_283108201.1 IS3 family transposase -
  C0971_RS16990 (C0971_17015) - 3332460..3332912 (-) 453 WP_248892445.1 IS3 family transposase -
  C0971_RS16995 (C0971_17020) - 3332897..3333250 (-) 354 WP_248892446.1 transposase -
  C0971_RS17000 (C0971_17025) - 3333332..3333523 (+) 192 WP_248892447.1 transposase -
  C0971_RS17005 (C0971_17030) - 3333677..3335704 (-) 2028 WP_248892448.1 Ig-like domain-containing protein -
  C0971_RS17010 (C0971_17035) - 3335832..3336671 (-) 840 WP_248892449.1 tyrosine-type recombinase/integrase -
  C0971_RS17015 (C0971_17040) - 3336683..3337021 (-) 339 WP_248892450.1 tyrosine-type recombinase/integrase -
  C0971_RS17020 (C0971_17045) istA 3337053..3338243 (+) 1191 Protein_3310 IS21 family transposase -
  C0971_RS17025 (C0971_17050) istA 3338373..3339929 (+) 1557 WP_248892451.1 IS21 family transposase -
  C0971_RS17030 (C0971_17055) istB 3339926..3340696 (+) 771 WP_248892452.1 IS21-like element helper ATPase IstB -
  C0971_RS17035 (C0971_17060) istB 3340821..3341573 (+) 753 WP_248892453.1 IS21-like element helper ATPase IstB -
  C0971_RS17040 (C0971_17065) - 3341594..3342241 (-) 648 WP_248892454.1 tyrosine-type recombinase/integrase -
  C0971_RS17045 (C0971_17070) xerA 3342263..3343096 (-) 834 WP_248892455.1 site-specific tyrosine recombinase/integron integrase -
  C0971_RS17050 (C0971_17075) - 3343102..3343836 (-) 735 WP_248892456.1 tyrosine-type recombinase/integrase -
  C0971_RS17055 - 3343821..3343994 (-) 174 WP_248892457.1 hypothetical protein -
  C0971_RS17060 (C0971_17080) - 3344349..3344810 (+) 462 WP_248892458.1 hypothetical protein -
  C0971_RS17065 (C0971_17085) - 3345154..3347844 (-) 2691 WP_248892459.1 Ig-like domain-containing protein -
  C0971_RS17070 - 3348475..3349812 (-) 1338 WP_248892460.1 hypothetical protein -
  C0971_RS17075 - 3349799..3350674 (-) 876 WP_248892461.1 hypothetical protein -
  C0971_RS17080 (C0971_17095) - 3350711..3352096 (-) 1386 WP_248892462.1 S41 family peptidase -
  C0971_RS17085 (C0971_17100) - 3352315..3353148 (+) 834 WP_248892463.1 DUF4352 domain-containing protein -
  C0971_RS17090 (C0971_17105) - 3353401..3353847 (+) 447 WP_248892464.1 C40 family peptidase -
  C0971_RS17095 (C0971_17110) - 3354149..3355771 (+) 1623 WP_248892465.1 SWIM zinc finger family protein -
  C0971_RS17100 (C0971_17115) - 3355764..3358574 (+) 2811 WP_248892466.1 DEAD/DEAH box helicase -
  C0971_RS17105 (C0971_17120) - 3358767..3359195 (-) 429 WP_248892467.1 hypothetical protein -
  C0971_RS17110 (C0971_17130) - 3359449..3359649 (+) 201 WP_248892468.1 transposase -
  C0971_RS17115 (C0971_17135) - 3359676..3360071 (-) 396 WP_283108202.1 HXXEE domain-containing protein -
  C0971_RS17120 (C0971_17140) - 3360680..3362125 (-) 1446 WP_248892470.1 NADP-dependent glyceraldehyde-3-phosphate dehydrogenase -
  C0971_RS17125 (C0971_17145) - 3362529..3363212 (-) 684 WP_248892471.1 TerC family protein -
  C0971_RS17130 (C0971_17150) - 3363430..3363678 (-) 249 WP_248892472.1 hypothetical protein -
  C0971_RS17135 (C0971_17155) ssbB 3363665..3364042 (-) 378 WP_003346745.1 single-stranded DNA-binding protein Machinery gene
  C0971_RS17140 (C0971_17160) - 3364342..3364764 (+) 423 WP_248892473.1 YwpF-like family protein -
  C0971_RS17145 (C0971_17165) - 3364859..3365086 (-) 228 WP_248892474.1 hypothetical protein -
  C0971_RS17150 (C0971_17170) - 3365247..3365522 (-) 276 WP_003346753.1 hypothetical protein -
  C0971_RS17155 (C0971_17175) fabZ 3365642..3366076 (-) 435 WP_003346755.1 3-hydroxyacyl-ACP dehydratase FabZ -
  C0971_RS17160 (C0971_17180) - 3366230..3366511 (-) 282 WP_248892475.1 DNA-directed RNA polymerase subunit beta -
  C0971_RS17165 (C0971_17185) - 3366527..3367369 (-) 843 WP_248892476.1 flagellar hook-basal body protein -
  C0971_RS17170 (C0971_17190) - 3367420..3368259 (-) 840 WP_248892477.1 flagellar hook-basal body protein -
  C0971_RS17175 (C0971_17195) - 3368430..3369431 (-) 1002 WP_003346763.1 rod shape-determining protein -
  C0971_RS17180 (C0971_17200) spoIIID 3369936..3370208 (-) 273 WP_003346764.1 sporulation transcriptional regulator SpoIIID -
  C0971_RS17185 (C0971_17205) - 3370647..3371630 (-) 984 WP_248892478.1 M23 family metallopeptidase -

Sequence


Protein


Download         Length: 125 a.a.        Molecular weight: 14189.12 Da        Isoelectric Point: 9.4666

>NTDB_id=267539 C0971_RS17135 WP_003346745.1 3363665..3364042(-) (ssbB) [Bacillus methanolicus strain DFS2]
MINQVTLVGRLTRDPELRVTPDGTYVANIILAVNRNYRNQNGEIDTDFVQCTLWKKIAENTAQYCRKGSVLGITGRIQTR
NYENQEGKKVFVTEVVAEGVRFLSPKPSAEHTKQKEKEAFTVEKS

Nucleotide


Download         Length: 378 bp        

>NTDB_id=267539 C0971_RS17135 WP_003346745.1 3363665..3364042(-) (ssbB) [Bacillus methanolicus strain DFS2]
TTGATCAACCAAGTTACACTTGTCGGGAGGCTGACCAGAGACCCTGAATTAAGGGTTACACCCGATGGTACGTACGTAGC
CAATATCATTTTAGCGGTCAACCGCAACTATCGAAACCAGAATGGAGAAATCGACACTGACTTTGTCCAATGCACCCTCT
GGAAAAAAATCGCCGAGAACACGGCACAATATTGCAGGAAAGGTTCAGTGCTCGGAATCACAGGCAGAATCCAAACGAGA
AACTACGAAAATCAAGAAGGTAAAAAAGTTTTTGTAACAGAGGTCGTTGCTGAAGGTGTCCGATTCCTGAGCCCAAAGCC
TTCAGCTGAACATACCAAACAAAAAGAAAAGGAGGCTTTTACTGTTGAAAAATCATGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB I3EBG5

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbB Bacillus subtilis subsp. subtilis str. 168

63.551

85.6

0.544

  ssbA Bacillus subtilis subsp. subtilis str. 168

60.377

84.8

0.512

  ssb Latilactobacillus sakei subsp. sakei 23K

50.45

88.8

0.448

  ssbB/cilA Streptococcus mitis NCTC 12261

42.991

85.6

0.368

  ssbB/cilA Streptococcus pneumoniae TIGR4

42.991

85.6

0.368

  ssbB Streptococcus sobrinus strain NIDR 6715-7

42.453

84.8

0.36


Multiple sequence alignment