Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C0099_RS04430 Genome accession   NZ_CP025682
Coordinates   922120..923835 (-) Length   571 a.a.
NCBI ID   WP_102246324.1    Uniprot ID   A0A2I6S4R5
Organism   Pseudazoarcus pumilus strain SY39     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 917120..928835
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C0099_RS04410 (C0099_04410) aspS 917728..919527 (-) 1800 WP_102246321.1 aspartate--tRNA ligase -
  C0099_RS04415 (C0099_04415) - 919595..919876 (-) 282 WP_102246322.1 FmdB family zinc ribbon protein -
  C0099_RS04420 (C0099_04420) pilD 919969..920820 (-) 852 WP_102248379.1 A24 family peptidase Machinery gene
  C0099_RS04425 (C0099_04425) pilC 920867..922102 (-) 1236 WP_102246323.1 type II secretion system F family protein Machinery gene
  C0099_RS04430 (C0099_04430) pilB 922120..923835 (-) 1716 WP_102246324.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C0099_RS04435 (C0099_04435) - 923924..925219 (-) 1296 WP_102246325.1 HlyC/CorC family transporter -
  C0099_RS04440 (C0099_04440) - 925296..926114 (-) 819 WP_102246326.1 inner membrane protein YpjD -
  C0099_RS04445 (C0099_04445) ffh 926226..927584 (+) 1359 WP_102246327.1 signal recognition particle protein -
  C0099_RS04450 (C0099_04450) - 927593..928324 (+) 732 WP_102246328.1 EI24 domain-containing protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 61799.03 Da        Isoelectric Point: 5.3365

>NTDB_id=263220 C0099_RS04430 WP_102246324.1 922120..923835(-) (pilB) [Pseudazoarcus pumilus strain SY39]
MAANPQTPLSGLARALIQQGRVTEADAIACTEAAGNTTNGFIVELDRRAILKSLDIARFAADTFGCPLLDVAALDVTATP
KDAVDRKLLAKHQVVPLARRQNRLVIATADPSNMRALDEIRFQSGMQVEVVVAEAAKLAKIVEKLAESAEETLRELTGEG
IELDLLESVAGGESPSDEANQEVDDAPVVRYIQKILIDAINEGASDIHFEPYEKHYRIRVRTDGVLREVAQPPLVLKDKI
AARIKVISRLDISEKRLPQDGRMKLVLSKNKAIDFRVSSLPTLHGEKIVMRILDSASALMGIDALGYEPDQKKALLEAIK
RPYGMILVTGPTGSGKTVSLYTCLNILNTAGTNISTAEDPAEINIAGINQVNIHEKAGLTFAAALRAFLRQDPDIIMVGE
IRDLETAEIAIKAAQTGHLMLSTLHTNDAPSTLERLRNMGVAPFNIASSVVLISAQRLARRLCSCKKPVDIPVEALLQAG
FPAEDIDGSWQPQGPVGCERCNGSGYRGRVGIYQVMPVSDEMARMIMTNCSSIDIADQARNEGVRDLRASGLIKVKQGIT
SLEEVLANTND

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=263220 C0099_RS04430 WP_102246324.1 922120..923835(-) (pilB) [Pseudazoarcus pumilus strain SY39]
ATGGCAGCCAATCCCCAGACTCCTCTCAGTGGCCTCGCGCGCGCACTGATCCAGCAGGGACGGGTGACCGAGGCGGACGC
CATCGCCTGCACCGAGGCGGCCGGCAATACGACCAACGGCTTCATCGTGGAGCTGGACCGGCGCGCCATCCTCAAGAGTC
TCGACATCGCCCGCTTCGCCGCCGACACCTTCGGCTGTCCGCTGCTCGACGTGGCCGCGCTCGACGTCACGGCCACGCCC
AAGGATGCGGTCGATCGCAAGCTGCTCGCCAAGCACCAGGTGGTACCGCTGGCCAGGCGCCAGAACCGGCTGGTGATTGC
CACGGCCGACCCGTCGAACATGCGCGCGCTCGACGAAATCCGCTTCCAGAGCGGCATGCAGGTGGAGGTGGTGGTCGCCG
AAGCGGCCAAGCTGGCGAAAATCGTCGAAAAGCTCGCCGAATCGGCCGAAGAGACGCTGCGCGAACTCACCGGCGAGGGC
ATCGAACTCGACCTGCTCGAGAGTGTCGCCGGCGGGGAGTCTCCATCGGATGAAGCCAACCAGGAGGTCGATGACGCTCC
GGTCGTGCGCTACATCCAGAAGATCCTGATCGACGCGATCAACGAGGGCGCCTCGGACATTCACTTCGAGCCCTACGAGA
AGCACTACCGCATCCGCGTGCGCACCGACGGCGTGCTGCGCGAGGTCGCCCAGCCGCCGCTGGTGCTCAAGGACAAGATC
GCCGCGCGCATCAAGGTGATCTCGCGGCTCGACATCTCGGAAAAGCGCCTGCCCCAGGACGGGCGCATGAAGCTGGTGCT
GTCCAAGAACAAGGCCATCGATTTCCGCGTTTCCTCGCTGCCGACCCTGCACGGCGAGAAGATCGTGATGCGTATCCTCG
ACTCGGCCTCGGCGCTGATGGGCATCGACGCGCTGGGCTACGAGCCGGACCAGAAGAAGGCGCTGCTCGAGGCCATCAAG
CGCCCCTACGGCATGATCCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTGTACACCTGCCTGAACATCCT
CAATACTGCCGGCACCAACATTTCCACCGCCGAGGACCCGGCCGAGATCAACATCGCGGGCATCAATCAGGTCAACATCC
ACGAAAAGGCCGGTCTGACCTTCGCCGCTGCGTTGCGCGCCTTCCTGCGTCAGGATCCGGACATCATCATGGTCGGCGAG
ATCCGTGACCTGGAAACGGCCGAGATCGCGATCAAGGCCGCGCAGACCGGCCACCTGATGCTTTCCACGCTGCACACCAA
CGATGCGCCCTCGACGCTCGAGCGCCTGCGCAACATGGGCGTGGCGCCGTTCAACATCGCCTCGTCGGTGGTGCTGATCT
CCGCCCAGCGCCTGGCGCGCCGGTTGTGCTCGTGCAAGAAGCCGGTCGACATCCCGGTCGAGGCGCTGCTGCAGGCCGGT
TTTCCGGCCGAGGACATTGACGGCAGCTGGCAGCCGCAGGGCCCGGTCGGCTGCGAGCGCTGCAACGGGTCGGGCTATCG
CGGCCGCGTCGGCATCTATCAGGTGATGCCGGTCTCCGACGAGATGGCGCGCATGATCATGACCAACTGCAGCTCGATCG
ACATCGCCGACCAGGCACGCAACGAAGGCGTGCGCGACCTGCGCGCCTCCGGCCTGATCAAGGTCAAGCAGGGCATCACC
TCGCTCGAGGAAGTGCTCGCGAACACCAACGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(16-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I6S4R5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.93

99.475

0.546
  pilB Acinetobacter baylyi ADP1

54.577

99.475

0.543
  pilF Neisseria gonorrhoeae MS11

53.004

99.124

0.525
  pilB Legionella pneumophila strain ERS1305867

52.557

99.299

0.522
  pilB Vibrio cholerae strain A1552

49.726

95.797

0.476
  pilB Vibrio parahaemolyticus RIMD 2210633

48.307

98.249

0.475
  pilB Vibrio campbellii strain DS40M4

46.263

98.424

0.455
  pilF Thermus thermophilus HB27

43.265

85.814

0.371

Multiple sequence alignment