Detailed information NTG0261283

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: CXB49_RS20640
• Genome accession: NZ_CP025429
• Coordinates: 4633012..4634148 (+)
• Length: 378 a.a.
• NCBI ID: WP_101710108.1
• Uniprot ID: A0A2H5E146
• Organism: Chromobacterium sp. ATCC 53434
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4628012..4639148

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CXB49_RS20620 (CXB49_20620)	-	4628522..4629217 (-)	696	WP_101710104.1	YggS family pyridoxal phosphate-dependent enzyme	-
CXB49_RS20625 (CXB49_20625)	-	4629472..4629966 (+)	495	WP_101710105.1	molybdopterin-dependent oxidoreductase	-
CXB49_RS20630 (CXB49_20630)	-	4629989..4631710 (+)	1722	WP_101710106.1	hybrid sensor histidine kinase/response regulator	-
CXB49_RS20635 (CXB49_20635)	pilT	4631851..4632888 (+)	1038	WP_101710107.1	type IV pilus twitching motility protein PilT	Machinery gene
CXB49_RS20640 (CXB49_20640)	pilU	4633012..4634148 (+)	1137	WP_101710108.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
CXB49_RS20645 (CXB49_20645)	-	4634174..4635787 (+)	1614	WP_101710109.1	response regulator	-
CXB49_RS20650 (CXB49_20650)	-	4635865..4637478 (+)	1614	WP_101710110.1	response regulator	-
CXB49_RS20655 (CXB49_20655)	-	4637508..4638251 (-)	744	WP_233492873.1	TIGR00266 family protein	-
CXB49_RS20660 (CXB49_20660)	moaE	4638248..4638721 (-)	474	WP_101710112.1	molybdopterin synthase catalytic subunit MoaE	-
CXB49_RS20665 (CXB49_20665)	moaD	4638723..4638974 (-)	252	WP_101710113.1	molybdopterin converting factor subunit 1	-

Sequence

Protein

• Length: 378 a.a.
• Molecular weight: 42299.38 Da
• Isoelectric Point: 6.0172

>NTDB_id=261283 CXB49_RS20640 WP_101710108.1 4633012..4634148(+) (pilU) [Chromobacterium sp. ATCC 53434]
MEKDQASKFIHDLLKHAIGKNASDVFISPDFPPAMKIDGKITPVAPQPLSAQQSKELVRAIMNDRQTEEFETRKEANFAI
NPPGIGRFRVSAFVQQGAVGMVLRKINTDIPNFDQLNLPEVLKDIALIKRGLVIFVGGTGSGKSTSLAALVDWRNSTNQD
HIITVEDPIEYVHAHKKSIVTQREIGVDTESWEIALKNTLRQAPDVILMGEIRDRETMGYGLQFAETGHLCLATLHANNA
NQALDRILNFFPEERHQQVLMDLSLNMRSIISQRLVPHKSGRGRVAAVEILLNSPLVSDMIFKGEIAGLKEVMARSREAG
MQTFDQSLFELFEADLITYEDALRNADSINDLRLRIKLYSESGRNRDPLEGIDHLDIV

Nucleotide

• Length: 1137 bp

>NTDB_id=261283 CXB49_RS20640 WP_101710108.1 4633012..4634148(+) (pilU) [Chromobacterium sp. ATCC 53434]
ATGGAAAAAGACCAGGCATCCAAGTTCATCCACGATCTGCTCAAACACGCGATCGGCAAGAATGCCTCCGACGTTTTCAT
CTCTCCCGACTTCCCGCCGGCGATGAAGATAGACGGCAAGATCACCCCGGTGGCGCCGCAGCCGCTGTCGGCCCAGCAAA
GCAAGGAATTGGTGCGGGCGATCATGAACGATCGCCAGACCGAGGAGTTCGAGACCCGCAAGGAAGCCAATTTCGCGATC
AATCCACCCGGCATCGGCCGCTTCCGCGTCAGCGCCTTCGTCCAGCAGGGCGCCGTCGGCATGGTGCTGCGCAAGATCAA
CACCGACATCCCCAACTTCGACCAGCTGAATCTGCCCGAGGTGCTGAAGGACATCGCGCTGATCAAGCGCGGGCTGGTGA
TCTTCGTCGGCGGCACCGGCTCGGGCAAGTCCACCTCGCTGGCCGCGCTGGTGGACTGGCGCAACAGCACCAACCAGGAT
CACATCATCACCGTCGAGGATCCGATCGAGTACGTGCACGCCCACAAGAAGTCCATCGTCACCCAGCGCGAGATCGGCGT
CGACACCGAGAGCTGGGAGATCGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATACTGATGGGCGAGATCCGCG
ACCGCGAGACCATGGGCTACGGCCTGCAGTTCGCCGAGACCGGCCACCTGTGTCTGGCCACCTTGCATGCCAACAACGCC
AACCAGGCGCTGGACCGCATCCTCAACTTCTTCCCCGAGGAACGCCACCAGCAGGTGCTGATGGACCTGTCGCTGAACAT
GCGCTCCATCATTTCGCAGCGGCTGGTGCCGCACAAATCCGGCCGCGGCCGGGTGGCCGCGGTGGAAATCCTGTTGAACA
GCCCGCTGGTATCGGACATGATCTTCAAAGGCGAGATCGCCGGCCTGAAGGAAGTGATGGCCCGCTCGCGCGAAGCCGGC
ATGCAAACCTTCGACCAGTCGCTGTTCGAGCTGTTCGAGGCCGATTTGATCACCTACGAGGACGCGCTGCGCAACGCCGA
CTCCATCAACGACCTGCGCCTGAGAATCAAGCTGTACAGCGAAAGCGGCCGCAACCGCGACCCGCTGGAAGGCATCGACC
ACCTGGATATCGTCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (14-277)

3D structure

• AlphaFold DB: A0A2H5E146

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	60	92.593	0.556
pilU	Acinetobacter baylyi ADP1	54.918	96.825	0.532
pilU	Vibrio cholerae strain A1552	53.846	92.857	0.5
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	44.214	89.153	0.394
pilT	Pseudomonas aeruginosa PAK	41.246	89.153	0.368