Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CSC46_RS14750 Genome accession   NZ_CP030351
Coordinates   3126788..3128197 (-) Length   469 a.a.
NCBI ID   WP_003112722.1    Uniprot ID   Q9I5N9
Organism   Pseudomonas aeruginosa strain AR_460     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3121788..3133197
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CSC46_RS14735 (CSC46_2983) - 3122756..3123868 (-) 1113 WP_003112720.1 PstS family phosphate ABC transporter substrate-binding protein -
  CSC46_RS14740 (CSC46_2984) lapA 3123954..3125060 (-) 1107 WP_003112123.1 alkaline phosphatase LapA -
  CSC46_RS14745 (CSC46_2985) pilC 3125574..3126788 (-) 1215 WP_003112721.1 type II secretion system inner membrane protein GspF Machinery gene
  CSC46_RS14750 (CSC46_2986) pilB 3126788..3128197 (-) 1410 WP_003112722.1 type II secretion system ATPase GspE Machinery gene
  CSC46_RS14755 (CSC46_2987) gspD 3128194..3130598 (-) 2405 Protein_2851 type II secretion system secretin GspD -
  CSC46_RS14760 (CSC46_2989) gspM 3130595..3131188 (-) 594 WP_010793838.1 type II secretion system protein GspM -
  CSC46_RS14765 (CSC46_2990) gspL 3131175..3132386 (-) 1212 WP_016253830.1 type II secretion system protein GspL -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 50978.36 Da        Isoelectric Point: 5.7943

>NTDB_id=260149 CSC46_RS14750 WP_003112722.1 3126788..3128197(-) (pilB) [Pseudomonas aeruginosa strain AR_460]
MSLLPYAWAKAQRALLRPGEHGATLLVSPRTPGWAISEVRQRHAPASLESVRDDELDTLLASAYSDTGSAAAVVGAAESE
VDLDRLMDDIPEVTDLLDTQDGAPVIRMINALLTQAARDEASDIHIEPFETHSVVRYRVDGALRDVVAPRKALHAALVSR
IKIMAQLDIAEKRLPQDGRIALRVAGRPIDIRVSTVPTGHGERVVMRLLDKQAGRLRLETLGMAPGVLAPLDNLIRQPHG
IVLVTGPTGSGKTTTLYAALARLDASTSNILTVEDPVEYDLPGISQIQVNARIDMTFAVALRAILRQDPDIIMIGEIRDL
ETAQIAVQASLTGHLVLATLHTNDAVSAVTRLVDMGVEPFLLASSMLGVLAQRLVRRLCTHCRVEEDGGWRAVGCPACNQ
TGYSGRTGIHELFVIDDEIRRLVHQGRAEQDLREAARAAGMRSMREDGERWIASGSTTLEEILRVTRDA

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=260149 CSC46_RS14750 WP_003112722.1 3126788..3128197(-) (pilB) [Pseudomonas aeruginosa strain AR_460]
ATGAGCCTGCTGCCCTACGCCTGGGCCAAGGCGCAGCGCGCCTTGCTGCGGCCGGGCGAGCATGGCGCGACCCTGCTGGT
CAGCCCGCGCACGCCCGGCTGGGCGATCAGCGAGGTGCGCCAGCGGCATGCGCCGGCCAGCCTGGAGAGCGTCCGCGACG
ACGAGCTCGACACCCTGCTGGCCAGCGCCTACTCCGATACCGGCAGCGCCGCGGCGGTGGTCGGCGCGGCGGAGAGCGAG
GTCGACCTCGACCGCCTGATGGACGATATCCCCGAGGTTACCGACCTGCTCGACACCCAGGATGGCGCGCCAGTGATCCG
CATGATCAACGCGCTGCTGACCCAGGCCGCGCGCGATGAGGCCAGCGACATCCACATCGAGCCCTTCGAGACCCATTCGG
TGGTGCGCTACCGCGTCGACGGCGCCCTGCGCGACGTGGTCGCGCCGCGCAAGGCGCTGCACGCGGCGCTGGTGTCGCGG
ATCAAGATCATGGCGCAGCTGGATATCGCTGAGAAACGCCTGCCCCAGGACGGCCGCATCGCCTTGCGGGTAGCCGGGCG
GCCGATCGACATTCGCGTCTCCACCGTGCCCACCGGCCACGGCGAACGGGTGGTGATGCGCCTGCTCGACAAGCAGGCCG
GACGCCTGAGGCTGGAGACCCTGGGCATGGCTCCCGGCGTGCTGGCGCCGCTGGACAACCTGATCCGCCAGCCGCACGGC
ATCGTGCTGGTCACCGGGCCCACCGGCAGCGGCAAGACCACCACCCTCTACGCCGCCCTGGCGCGGCTCGACGCCAGCAC
CAGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGACCTGCCGGGGATCAGCCAGATCCAGGTCAACGCGCGCATCG
ACATGACCTTCGCCGTAGCCCTGCGGGCGATCCTCCGGCAGGACCCGGACATCATCATGATCGGCGAGATCCGCGACCTG
GAGACCGCGCAGATCGCCGTGCAGGCCTCGCTCACCGGGCACCTGGTGCTGGCCACGCTGCACACCAACGACGCGGTGTC
GGCAGTGACGCGGCTGGTGGACATGGGCGTCGAGCCGTTCCTGCTCGCCTCGTCGATGCTTGGCGTACTCGCCCAGCGCC
TGGTGCGGCGCCTGTGCACGCATTGCCGGGTCGAAGAGGACGGCGGCTGGCGCGCGGTCGGCTGCCCGGCTTGCAACCAG
ACCGGCTACAGCGGGCGCACCGGCATCCACGAACTGTTCGTGATCGACGACGAGATCCGCCGCCTGGTCCACCAGGGCCG
CGCCGAGCAGGATCTCCGCGAAGCCGCCCGCGCCGCCGGCATGCGCAGCATGCGCGAGGACGGCGAGCGCTGGATCGCCA
GCGGCTCCACCACCCTGGAGGAAATCCTCCGCGTGACCCGGGACGCCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(111-377)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9I5N9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

45.519

90.405

0.412
  pilF Thermus thermophilus HB27

50.928

80.384

0.409
  pilB/pilB1 Synechocystis sp. PCC 6803

41.113

99.574

0.409
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.462

83.156

0.403
  pilF Neisseria gonorrhoeae MS11

47.208

84.009

0.397
  pilB Acinetobacter baumannii D1279779

41.403

94.243

0.39
  pilB Vibrio campbellii strain DS40M4

46.329

84.222

0.39
  pilB Vibrio parahaemolyticus RIMD 2210633

46.076

84.222

0.388
  pilB Legionella pneumophila strain ERS1305867

45.501

82.942

0.377
  pilB Acinetobacter baylyi ADP1

45.103

82.729

0.373

Multiple sequence alignment