Detailed information NTG0259553

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: CXL14_RS10875
• Genome accession: NZ_CP025258
• Coordinates: 2124282..2124896 (+)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus sp. SJ-10
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2119282..2129896

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CXL14_RS10840	aroE	2119969..2120811 (+)	843	WP_003152856.1	shikimate dehydrogenase	-
CXL14_RS10845	yhbY	2120805..2121095 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
CXL14_RS10850	-	2121106..2121675 (+)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
CXL14_RS10855	yqeK	2121665..2122225 (+)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
CXL14_RS10860	rsfS	2122243..2122599 (+)	357	WP_007408260.1	ribosome silencing factor	-
CXL14_RS10865	-	2122596..2123333 (+)	738	WP_014418421.1	class I SAM-dependent methyltransferase	-
CXL14_RS10870	comER	2123402..2124223 (-)	822	WP_014418420.1	late competence protein ComER	-
CXL14_RS10875	comEA	2124282..2124896 (+)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
CXL14_RS10880	-	2124963..2125532 (+)	570	WP_003152868.1	ComE operon protein 2	-
CXL14_RS10885	comEC	2125533..2127884 (+)	2352	WP_014418418.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
CXL14_RS10890	-	2127903..2128037 (-)	135	WP_003152870.1	YqzM family protein	-
CXL14_RS20075	-	2128078..2128229 (+)	152	Protein_2070	hypothetical protein	-
CXL14_RS10895	holA	2128269..2129310 (+)	1042	Protein_2071	DNA polymerase III subunit delta	-
CXL14_RS10900	rpsT	2129327..2129593 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=259553 CXL14_RS10875 WP_014418419.1 2124282..2124896(+) (comEA) [Bacillus sp. SJ-10]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=259553 CXL14_RS10875 WP_014418419.1 2124282..2124896(+) (comEA) [Bacillus sp. SJ-10]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363