Detailed information NTG0258216

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: BVQ_RS13480
• Genome accession: NZ_CP025079
• Coordinates: 2709254..2709868 (-)
• Length: 204 a.a.
• NCBI ID: WP_053573180.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain QST713
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2704254..2714868

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BVQ_RS13455 (BVQ_13445)	rpsT	2704554..2704820 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
BVQ_RS13460 (BVQ_13450)	holA	2704837..2705878 (-)	1042	Protein_2607	DNA polymerase III subunit delta	-
BVQ_RS21420	-	2705918..2706072 (-)	155	Protein_2608	hypothetical protein	-
BVQ_RS13465 (BVQ_13455)	-	2706113..2706247 (+)	135	WP_003152870.1	YqzM family protein	-
BVQ_RS13470 (BVQ_13460)	comEC	2706266..2708617 (-)	2352	WP_053573181.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
BVQ_RS13475 (BVQ_13465)	-	2708618..2709187 (-)	570	WP_003152868.1	ComE operon protein 2	-
BVQ_RS13480 (BVQ_13470)	comEA	2709254..2709868 (-)	615	WP_053573180.1	helix-hairpin-helix domain-containing protein	Machinery gene
BVQ_RS13485 (BVQ_13475)	comER	2709927..2710748 (+)	822	WP_015417855.1	late competence protein ComER	-
BVQ_RS13490 (BVQ_13480)	-	2710817..2711554 (-)	738	WP_015417856.1	class I SAM-dependent DNA methyltransferase	-
BVQ_RS13495 (BVQ_13485)	rsfS	2711551..2711907 (-)	357	WP_007408260.1	ribosome silencing factor	-
BVQ_RS13500 (BVQ_13490)	yqeK	2711925..2712485 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
BVQ_RS13505 (BVQ_13495)	-	2712475..2713044 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
BVQ_RS13510 (BVQ_13500)	yhbY	2713055..2713345 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
BVQ_RS13515 (BVQ_13505)	aroE	2713339..2714181 (-)	843	WP_039254404.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22183.34 Da
• Isoelectric Point: 6.4888

>NTDB_id=258216 BVQ_RS13480 WP_053573180.1 2709254..2709868(-) (comEA) [Bacillus velezensis strain QST713]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTMVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=258216 BVQ_RS13480 WP_053573180.1 2709254..2709868(-) (comEA) [Bacillus velezensis strain QST713]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTTGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATGGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.328	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363