Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PCRYO_RS00350 Genome accession   NC_007969
Coordinates   77937..79649 (-) Length   570 a.a.
NCBI ID   WP_011512444.1    Uniprot ID   Q1QEQ0
Organism   Psychrobacter cryohalolentis K5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 72937..84649
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCRYO_RS00325 (Pcryo_0064) - 73502..73837 (+) 336 WP_011512439.1 pyrimidine/purine nucleoside phosphorylase -
  PCRYO_RS00330 (Pcryo_0065) rlmB 74042..74812 (+) 771 WP_011512440.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  PCRYO_RS00335 (Pcryo_0066) coaE 74775..75467 (-) 693 WP_011512441.1 dephospho-CoA kinase -
  PCRYO_RS00340 (Pcryo_0067) pilD 75551..76450 (-) 900 WP_011512442.1 prepilin peptidase Machinery gene
  PCRYO_RS00345 (Pcryo_0068) pilC 76476..77699 (-) 1224 WP_011512443.1 type II secretion system F family protein Machinery gene
  PCRYO_RS00350 (Pcryo_0069) pilB 77937..79649 (-) 1713 WP_011512444.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PCRYO_RS00355 (Pcryo_0070) tpiA 80047..80856 (+) 810 WP_011512445.1 triose-phosphate isomerase -
  PCRYO_RS00360 (Pcryo_0071) secG 81085..81393 (+) 309 WP_011512446.1 preprotein translocase subunit SecG -
  PCRYO_RS00375 (Pcryo_0072) rimP 82297..82797 (+) 501 WP_011512447.1 ribosome maturation factor RimP -
  PCRYO_RS00380 (Pcryo_0073) nusA 82931..84415 (+) 1485 WP_011512448.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63160.51 Da        Isoelectric Point: 5.3322

>NTDB_id=25760 PCRYO_RS00350 WP_011512444.1 77937..79649(-) (pilB) [Psychrobacter cryohalolentis K5]
MSITTSKYGGLAQQLISEGIVSEAHMKAAQTDSQQQQVGLVSYLVEHKLANAYQLAQMLSQAFGDPLFDLDTLSSEVIPK
DLVDEKIVRKFNALPMFKRGQRLFVALSDPTRIDAIDAIAFNSRLSIETVVVEEDKLKRRIESFYADSMQNFDSFSNSDL
HIGFDESQEEDDGETKISDSVEEAPVVKFVNKMLVDAIRMGASDLHFEPYEKSYRVRFRVDGVMQKMANPPVQLAGKIAA
RLKVMSQMDISERRVPQDGRIKLKISKNKAIDFRVSCLPTLFGEKLVLRILDPSSAMLGIEALGYEPDQQDMFLEALHKP
QGMLLITGPTGSGKTVSLYTGINILNTGSTNISTAEDPVEINLEGINQVNVNPKVGLTFSTALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVASFNIATSVNLVIAQRLARRLCKNCKKPISIPRQSLLELGF
TDADLDNTDNIIYEPVGCNECREGYKGRVGIYEVMKVTPDISRIIMEDGNAIDIKDAALKNGFRDLRRSGVLKVLQGVTS
IQEMMRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=25760 PCRYO_RS00350 WP_011512444.1 77937..79649(-) (pilB) [Psychrobacter cryohalolentis K5]
ATGTCTATCACAACCAGTAAATACGGTGGCTTAGCACAGCAGCTGATTAGCGAAGGTATCGTAAGCGAAGCGCATATGAA
AGCGGCGCAGACTGATTCGCAGCAGCAGCAAGTAGGACTGGTCTCTTATCTGGTAGAGCATAAGCTTGCCAATGCCTATC
AATTGGCTCAAATGCTATCGCAAGCATTCGGCGATCCTTTATTTGATCTTGATACGCTCAGCTCAGAGGTCATCCCAAAA
GACTTGGTCGACGAAAAAATCGTCCGTAAGTTTAATGCCTTGCCAATGTTTAAGCGTGGGCAACGCTTATTTGTGGCACT
GAGTGATCCGACCCGTATCGATGCGATTGATGCGATTGCCTTTAACTCTCGCTTGTCAATTGAAACCGTTGTAGTCGAAG
AAGATAAGCTTAAAAGGCGTATTGAAAGCTTCTATGCTGATAGCATGCAAAATTTTGATAGCTTTTCTAATAGTGATTTG
CATATTGGCTTTGATGAGAGTCAAGAAGAAGATGACGGCGAGACCAAAATCTCTGATAGCGTCGAGGAAGCACCGGTCGT
CAAGTTTGTTAATAAAATGCTAGTAGATGCCATTCGTATGGGTGCGTCCGACTTACATTTTGAGCCCTATGAAAAAAGCT
ATCGAGTGCGCTTTCGAGTCGACGGTGTGATGCAAAAAATGGCCAACCCGCCCGTACAACTCGCGGGGAAAATTGCTGCG
CGTTTAAAAGTAATGTCACAAATGGATATCTCAGAGCGCCGTGTCCCTCAAGATGGACGTATTAAGCTAAAGATATCTAA
AAATAAGGCCATCGATTTTCGGGTAAGTTGCCTACCTACCTTGTTTGGCGAAAAACTGGTTTTGCGTATTCTAGATCCCT
CATCAGCCATGCTTGGTATTGAAGCGTTGGGCTATGAGCCTGACCAACAAGATATGTTTTTGGAGGCGTTACATAAACCG
CAAGGCATGCTCCTAATTACTGGTCCCACTGGTTCTGGTAAAACCGTCTCACTCTATACTGGTATTAACATCCTGAATAC
TGGTTCGACCAATATCTCGACTGCCGAAGATCCTGTCGAAATTAACCTTGAAGGTATTAATCAGGTCAACGTCAACCCAA
AAGTGGGGCTGACTTTCTCCACCGCCCTTAAGTCTTTCTTACGCCAAGATCCTGATATTGTGATGGTTGGTGAGATTCGT
GATCTTGAAACTGCTGAGATTGCGATTAAAGCAGCACAGACAGGGCACATGGTACTTTCAACCTTACATACCAATTCAGC
GCCTGAAACGTTAACGCGATTACGCAATATGGGTGTCGCTTCTTTTAATATTGCCACTTCCGTGAACTTGGTCATTGCTC
AGCGATTGGCACGGCGCTTATGTAAAAACTGTAAAAAACCCATTAGTATCCCACGTCAAAGTCTGCTTGAGCTTGGTTTT
ACAGATGCAGATTTGGACAATACAGACAATATCATCTACGAGCCAGTCGGTTGTAATGAGTGCCGTGAAGGATACAAAGG
ACGTGTTGGTATTTATGAGGTGATGAAAGTCACTCCAGATATTTCACGTATTATCATGGAAGATGGTAACGCGATAGATA
TCAAAGATGCGGCGCTAAAAAACGGTTTTAGAGACTTACGTCGCTCTGGTGTTTTAAAGGTTTTACAAGGCGTCACCAGT
ATTCAAGAAATGATGCGCGTGACTTCTGAGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q1QEQ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

67.895

100

0.679
  pilB Acinetobacter baylyi ADP1

63.908

99.649

0.637
  pilB Legionella pneumophila strain ERS1305867

51.473

100

0.521
  pilF Neisseria gonorrhoeae MS11

48.592

99.649

0.484
  pilB Vibrio cholerae strain A1552

50.847

93.158

0.474
  pilB Vibrio parahaemolyticus RIMD 2210633

51.238

92.105

0.472
  pilB Vibrio campbellii strain DS40M4

49.159

93.86

0.461
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.12

100

0.379

Multiple sequence alignment