Detailed information NTG0025758

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PCRYO_RS00340
• Genome accession: NC_007969
• Coordinates: 75551..76450 (-)
• Length: 299 a.a.
• NCBI ID: WP_011512442.1
• Uniprot ID: Q1QEQ2
• Organism: Psychrobacter cryohalolentis K5
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 70551..81450

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCRYO_RS00315 (Pcryo_0062)	-	70579..71142 (-)	564	WP_011512437.1	YqgE/AlgH family protein	-
PCRYO_RS00320 (Pcryo_0063)	recN	71273..72952 (-)	1680	WP_011512438.1	DNA repair protein RecN	-
PCRYO_RS00325 (Pcryo_0064)	-	73502..73837 (+)	336	WP_011512439.1	pyrimidine/purine nucleoside phosphorylase	-
PCRYO_RS00330 (Pcryo_0065)	rlmB	74042..74812 (+)	771	WP_011512440.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
PCRYO_RS00335 (Pcryo_0066)	coaE	74775..75467 (-)	693	WP_011512441.1	dephospho-CoA kinase	-
PCRYO_RS00340 (Pcryo_0067)	pilD	75551..76450 (-)	900	WP_011512442.1	prepilin peptidase	Machinery gene
PCRYO_RS00345 (Pcryo_0068)	pilC	76476..77699 (-)	1224	WP_011512443.1	type II secretion system F family protein	Machinery gene
PCRYO_RS00350 (Pcryo_0069)	pilB	77937..79649 (-)	1713	WP_011512444.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PCRYO_RS00355 (Pcryo_0070)	tpiA	80047..80856 (+)	810	WP_011512445.1	triose-phosphate isomerase	-
PCRYO_RS00360 (Pcryo_0071)	secG	81085..81393 (+)	309	WP_011512446.1	preprotein translocase subunit SecG	-

Sequence

Protein

• Length: 299 a.a.
• Molecular weight: 33074.81 Da
• Isoelectric Point: 8.6950

>NTDB_id=25758 PCRYO_RS00340 WP_011512442.1 75551..76450(-) (pilD) [Psychrobacter cryohalolentis K5]
MQFIQLLQENMTIALVVFGLLGLCVGSFLNVVIHRTPLIMVSAWRKECSQFMYEQADMPREHTMPLVNIVATDTPITLSK
PASRCPHCAHKIKWYENIPLISWIVLRGRCSECKAAIGLRYPIVELVTALLSALVIYHFGVNMVGLSALVLVWTLIALTG
IDFDTQLLPDRLTFPLAGLGLAVNSQGWFVPPTQSIWGLLLGFLSLWIVVKIFYLITKKHGMGQGDFKLLAVLGAWLGPM
MLPLIILLSSLLGSIVGLILMKKQGESKPFAFGPYIAIAGIVALLYGSDIVNWYLGMYT

Nucleotide

• Length: 900 bp

>NTDB_id=25758 PCRYO_RS00340 WP_011512442.1 75551..76450(-) (pilD) [Psychrobacter cryohalolentis K5]
ATGCAATTTATACAGTTACTACAAGAAAATATGACCATTGCTTTGGTCGTATTTGGTCTACTAGGGCTGTGCGTCGGTAG
CTTCTTAAACGTGGTAATTCATCGCACTCCATTGATAATGGTATCGGCTTGGCGAAAAGAGTGTAGCCAGTTTATGTATG
AGCAAGCAGATATGCCGCGTGAGCATACGATGCCACTAGTTAATATTGTTGCCACTGATACACCTATTACCTTAAGTAAA
CCAGCATCGCGCTGCCCGCACTGTGCCCACAAAATTAAGTGGTATGAAAACATACCTTTAATTAGCTGGATAGTACTACG
CGGACGTTGCTCAGAGTGCAAAGCGGCTATCGGGCTTCGATATCCTATCGTTGAGCTGGTCACTGCTCTGCTATCTGCAC
TAGTCATTTATCATTTTGGCGTTAATATGGTAGGGCTGTCGGCATTAGTTTTAGTCTGGACACTCATCGCCTTAACTGGT
ATCGATTTTGATACTCAGTTATTGCCTGACCGCTTAACTTTTCCATTAGCAGGGCTAGGATTGGCGGTCAATTCACAAGG
TTGGTTTGTGCCCCCTACTCAGTCGATATGGGGCTTGTTATTGGGATTTTTATCGTTATGGATAGTGGTCAAGATATTTT
ATTTAATCACCAAAAAGCATGGCATGGGACAAGGCGACTTTAAATTATTGGCAGTACTGGGTGCGTGGCTTGGACCTATG
ATGTTACCCTTGATTATTTTATTATCATCATTGCTCGGCTCTATCGTTGGGCTTATCTTGATGAAAAAACAAGGCGAGAG
CAAGCCTTTTGCCTTTGGTCCTTATATTGCCATTGCTGGTATTGTGGCATTGTTATACGGCTCAGATATCGTTAATTGGT
ATTTAGGTATGTATACTTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (149-258)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-139)

3D structure

• AlphaFold DB: Q1QEQ2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter nosocomialis M2	54.514	96.321	0.525
pilD	Acinetobacter baumannii D1279779	54.325	96.656	0.525
pilD	Vibrio campbellii strain DS40M4	48.084	95.987	0.462
pilD	Vibrio cholerae strain A1552	48.571	93.645	0.455
pilD	Neisseria gonorrhoeae MS11	40.714	93.645	0.381