Detailed information NTG0255511

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: CUB85_RS13125
• Genome accession: NZ_CP024797
• Coordinates: 2654175..2654789 (-)
• Length: 204 a.a.
• NCBI ID: WP_043021637.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain TJ02
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2649175..2659789

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CUB85_RS13100	rpsT	2649478..2649744 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
CUB85_RS13105	holA	2649761..2650802 (-)	1042	Protein_2517	DNA polymerase III subunit delta	-
CUB85_RS20505	-	2650837..2650993 (-)	157	Protein_2518	hypothetical protein	-
CUB85_RS13110	-	2651034..2651168 (+)	135	WP_003152870.1	YqzM family protein	-
CUB85_RS13115	comEC	2651187..2653538 (-)	2352	WP_094031847.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
CUB85_RS13120	-	2653539..2654108 (-)	570	WP_094031848.1	ComE operon protein 2	-
CUB85_RS13125	comEA	2654175..2654789 (-)	615	WP_043021637.1	helix-hairpin-helix domain-containing protein	Machinery gene
CUB85_RS13130	comER	2654848..2655669 (+)	822	WP_012118027.1	late competence protein ComER	-
CUB85_RS13135	-	2655738..2656475 (-)	738	WP_076424998.1	class I SAM-dependent DNA methyltransferase	-
CUB85_RS13140	rsfS	2656472..2656828 (-)	357	WP_007408260.1	ribosome silencing factor	-
CUB85_RS13145	yqeK	2656846..2657406 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
CUB85_RS13150	-	2657396..2657965 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
CUB85_RS13155	yhbY	2657976..2658266 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
CUB85_RS13160	aroE	2658260..2659102 (-)	843	WP_094031849.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22165.31 Da
• Isoelectric Point: 6.4888

>NTDB_id=255511 CUB85_RS13125 WP_043021637.1 2654175..2654789(-) (comEA) [Bacillus velezensis strain TJ02]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=255511 CUB85_RS13125 WP_043021637.1 2654175..2654789(-) (comEA) [Bacillus velezensis strain TJ02]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363