Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CTT31_RS03975 Genome accession   NZ_CP024625
Coordinates   944732..946408 (-) Length   558 a.a.
NCBI ID   WP_130164712.1    Uniprot ID   -
Organism   Pseudoalteromonas maricaloris strain SCSIO 43202     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 939732..951408
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CTT31_RS03950 (CTT31_03950) kdsA 940448..941302 (+) 855 WP_010370475.1 3-deoxy-8-phosphooctulonate synthase -
  CTT31_RS03955 (CTT31_03955) - 941388..942293 (+) 906 WP_088531408.1 transcriptional regulator GcvA -
  CTT31_RS03960 (CTT31_03960) - 942290..942928 (+) 639 WP_045989837.1 alpha/beta family hydrolase -
  CTT31_RS03965 (CTT31_03965) - 942921..943289 (+) 369 WP_130126782.1 DUF423 domain-containing protein -
  CTT31_RS03970 (CTT31_03970) rlmM 943303..944388 (+) 1086 WP_010607344.1 23S rRNA (cytidine(2498)-2'-O)-methyltransferase RlmM -
  CTT31_RS03975 (CTT31_03975) pilB 944732..946408 (-) 1677 WP_130164712.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CTT31_RS03980 (CTT31_03980) coaE 946504..947133 (-) 630 WP_260492009.1 dephospho-CoA kinase -
  CTT31_RS03985 (CTT31_03985) pilD 947143..948021 (-) 879 WP_260492010.1 prepilin peptidase Machinery gene
  CTT31_RS03990 (CTT31_03990) pilC 948145..949380 (-) 1236 WP_260492011.1 type II secretion system F family protein Machinery gene
  CTT31_RS03995 (CTT31_03995) - 949435..949872 (-) 438 WP_260492012.1 pilin -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 61997.27 Da        Isoelectric Point: 5.5806

>NTDB_id=254481 CTT31_RS03975 WP_130164712.1 944732..946408(-) (pilB) [Pseudoalteromonas maricaloris strain SCSIO 43202]
MEHHSPLLRKFITLGRVTAEQIKSRQPEANTTAELICLSSGMSSKELAEECLDLFKVPFFNLDHFNVSEIPPALVKEKLI
RKHHILPLVQKGRKLYVAASDPTDFGAFENFEFSTGLQCEVLVADYKKLEAKIDQLFDATGGLSISEEEFKEFADLEVDD
EPKQQNTNEDKDDAPIIVYINKILMDAIKKGASDLHFEPYEKKYRVRFRIDGLLHEMASPPNTLATRLAARIKVMAHLDI
AEKRKPQDGRIKLKISERKSIDFRVSTLPTMWGEKIVMRILDSSSAMLGIDVLGYEPEQKQLYLDALEQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGINQVQINTKADMTFANALRAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETLTRLLNMGVPAYNVASSVSLIIAQRLARRLCPKCKTPETLPEEALLGQGFTAEQIKEITL
FAPKGCESCTDGYKGRVGIYEVVKITPELSHLIMEGGNSLEIAEKAEQLGFDNLRKSGLKKAAAGVTSLTEINRVTSY

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=254481 CTT31_RS03975 WP_130164712.1 944732..946408(-) (pilB) [Pseudoalteromonas maricaloris strain SCSIO 43202]
ATGGAACATCATTCCCCGCTACTGCGTAAATTTATTACCTTAGGGCGAGTCACTGCTGAGCAAATAAAGTCTCGCCAGCC
AGAAGCAAACACCACCGCCGAGTTAATTTGCCTAAGTTCAGGAATGAGCTCCAAAGAGCTAGCTGAAGAATGCCTAGACT
TATTCAAGGTGCCATTTTTTAATCTTGATCACTTTAACGTTAGTGAAATTCCACCAGCGCTAGTCAAAGAAAAACTGATC
CGTAAGCACCATATTTTACCGCTGGTACAAAAAGGTAGAAAACTCTACGTTGCGGCTTCTGATCCTACTGACTTTGGCGC
ATTTGAAAACTTTGAGTTTAGTACTGGATTACAGTGTGAAGTACTGGTAGCCGACTACAAAAAACTCGAAGCAAAGATTG
ATCAGCTGTTTGATGCAACGGGCGGCCTCTCGATCTCTGAAGAAGAATTTAAAGAATTTGCCGACTTAGAAGTTGATGAC
GAGCCTAAACAGCAAAACACCAACGAAGACAAAGACGATGCGCCAATCATCGTTTATATCAATAAAATCTTAATGGACGC
GATAAAAAAAGGCGCGTCGGATCTACACTTTGAGCCCTACGAGAAGAAATATCGTGTGCGCTTTCGTATCGATGGCCTGC
TGCATGAGATGGCAAGCCCACCAAACACATTAGCTACTCGTCTCGCCGCCCGTATCAAAGTGATGGCGCATTTAGATATA
GCAGAAAAACGCAAACCGCAAGATGGCCGCATTAAATTAAAGATCTCCGAGCGCAAAAGTATCGATTTTCGTGTAAGTAC
ACTGCCTACCATGTGGGGCGAGAAAATCGTAATGCGTATTCTAGATTCGTCCAGCGCCATGCTCGGCATCGATGTGCTAG
GTTATGAACCAGAGCAGAAACAACTTTACCTCGACGCACTAGAGCAGCCTCAGGGCATGATTTTGGTAACCGGACCAACA
GGTTCCGGTAAAACCGTGTCACTTTACACTGGCTTGAATATTCTTAATCAGCCAGAGCGCAATATCAGCACGGCAGAAGA
CCCTGTCGAAATAAACCTTGAGGGTATTAATCAAGTACAAATCAATACCAAAGCGGATATGACTTTCGCCAATGCCTTGA
GAGCATTCTTGCGTCAGGATCCGGATGTGGTGATGGTTGGTGAGATCCGTGACCTTGAAACCGCTGAGATCTCAATAAAA
GCCGCCCAAACTGGTCACTTAGTATTGAGCACCCTGCACACCAACTCTGCGCCTGAAACGCTCACCCGTCTATTAAACAT
GGGCGTGCCGGCGTACAACGTAGCAAGCTCGGTGAGTTTGATTATTGCCCAGCGTCTCGCAAGACGTCTTTGTCCTAAGT
GTAAAACACCTGAAACGTTGCCGGAAGAAGCCTTGTTAGGGCAAGGCTTTACTGCCGAGCAAATTAAAGAGATTACCCTT
TTTGCACCTAAAGGCTGTGAAAGCTGCACCGATGGTTACAAAGGCCGTGTGGGTATATATGAAGTAGTAAAAATCACGCC
AGAGCTTTCGCACCTGATCATGGAAGGGGGCAATTCTCTTGAGATTGCTGAAAAAGCAGAGCAGTTAGGTTTTGATAACT
TACGTAAATCTGGCCTGAAAAAAGCCGCCGCAGGCGTAACATCGCTTACAGAAATCAACCGCGTAACGAGTTATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-140)

Type II/IV secretion system protein (T2SSE)

(182-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.895

100

0.591
  pilB Acinetobacter baylyi ADP1

57.27

100

0.579
  pilB Vibrio cholerae strain A1552

53.455

98.566

0.527
  pilB Vibrio parahaemolyticus RIMD 2210633

53.005

98.387

0.522
  pilB Vibrio campbellii strain DS40M4

52.545

98.566

0.518
  pilB Legionella pneumophila strain ERS1305867

54.894

93.369

0.513
  pilF Neisseria gonorrhoeae MS11

47.133

100

0.471
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.429

94.086

0.371
  pilF Thermus thermophilus HB27

36.396

99.462

0.362

Multiple sequence alignment