Detailed information NTG0252916

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: CS376_RS13300
• Genome accession: NZ_CP024203
• Coordinates: 2687989..2688603 (-)
• Length: 204 a.a.
• NCBI ID: WP_032866693.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain NKG-1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2682989..2693603

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CS376_RS13275	rpsT	2683293..2683559 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
CS376_RS13280	holA	2683576..2684617 (-)	1042	Protein_2567	DNA polymerase III subunit delta	-
CS376_RS21360	-	2684652..2684808 (-)	157	Protein_2568	hypothetical protein	-
CS376_RS13285	-	2684849..2684983 (+)	135	WP_003152870.1	YqzM family protein	-
CS376_RS13290	comEC	2685002..2687353 (-)	2352	WP_076424994.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
CS376_RS13295	-	2687354..2687922 (-)	569	Protein_2571	ComE operon protein 2	-
CS376_RS13300	comEA	2687989..2688603 (-)	615	WP_032866693.1	helix-hairpin-helix domain-containing protein	Machinery gene
CS376_RS13305	comER	2688662..2689483 (+)	822	WP_015240246.1	late competence protein ComER	-
CS376_RS13310	-	2689552..2690289 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
CS376_RS13315	rsfS	2690286..2690642 (-)	357	WP_007408260.1	ribosome silencing factor	-
CS376_RS13320	yqeK	2690660..2691220 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
CS376_RS13325	-	2691210..2691779 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
CS376_RS13330	yhbY	2691790..2692080 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
CS376_RS13335	aroE	2692074..2692916 (-)	843	WP_059368084.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22151.28 Da
• Isoelectric Point: 6.4858

>NTDB_id=252916 CS376_RS13300 WP_032866693.1 2687989..2688603(-) (comEA) [Bacillus velezensis strain NKG-1]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSDKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=252916 CS376_RS13300 WP_032866693.1 2687989..2688603(-) (comEA) [Bacillus velezensis strain NKG-1]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGACAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTATAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCAATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363