Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HCH_RS23705 Genome accession   NC_007645
Coordinates   5419902..5421614 (+) Length   570 a.a.
NCBI ID   WP_011399016.1    Uniprot ID   Q2SBM2
Organism   Hahella chejuensis KCTC 2396     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5414902..5426614
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HCH_RS23685 (HCH_05273) - 5415732..5416604 (-) 873 WP_011399012.1 glycosyltransferase family 2 protein -
  HCH_RS23690 (HCH_05275) - 5416601..5418478 (-) 1878 WP_041598890.1 hypothetical protein -
  HCH_RS33255 (HCH_05276) - 5418604..5419050 (-) 447 WP_011399014.1 pilin -
  HCH_RS23700 (HCH_05277) - 5419112..5419585 (-) 474 WP_011399015.1 pilin -
  HCH_RS23705 (HCH_05278) pilB 5419902..5421614 (+) 1713 WP_011399016.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HCH_RS23710 (HCH_05279) pilC 5421618..5422838 (+) 1221 WP_011399017.1 type II secretion system F family protein Machinery gene
  HCH_RS23715 (HCH_05280) pilD 5422905..5423783 (+) 879 WP_011399018.1 prepilin peptidase Machinery gene
  HCH_RS23720 (HCH_05281) coaE 5423803..5424429 (+) 627 WP_011399019.1 dephospho-CoA kinase -
  HCH_RS23725 (HCH_05282) yacG 5424419..5424655 (+) 237 WP_011399020.1 DNA gyrase inhibitor YacG -
  HCH_RS23730 (HCH_05283) - 5424706..5425746 (+) 1041 WP_011399021.1 hypothetical protein -
  HCH_RS23735 (HCH_05285) tsaA 5425756..5426475 (+) 720 WP_011399022.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62744.74 Da        Isoelectric Point: 5.4851

>NTDB_id=25204 HCH_RS23705 WP_011399016.1 5419902..5421614(+) (pilB) [Hahella chejuensis KCTC 2396]
MNTQMTTPLTGLARRFVQDGIIEENTAREAQNNAGKAKLPFVSYLVQQGLAKATQLAYAASAEFGVPLVDLNSVALDQIP
EKIVDEKLIRKHHALPLFKRGNRLFLALADPTNLQALDEIKFHTGLSTDAVLVEDDKLSTAIDRYLEAQEQPLGDIDGGD
LDDIDIETGDDSDKDDDSGAEQDDAPIVKYINKMLLDAIKGGASDIHFEPYEKTYRVRFRTDGILHEVSKPSIKLAAKLS
ARLKVMSQLDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSSAKLGIDALGYEEDQKKMYLDALAQ
PQGMILVTGPTGSGKTVSLYTGLNILNTTERNISTAEDPVEINLEGINQVNVNTKVGLDFSSALRAFLRQDPDVVMVGEI
RDLDTASIAIKAAQTGHLVLSTLHTNSAAETLTRLRNMGVPAFNIATSVSLIIAQRLARRLCAECKEVITVPKEVLKEEG
FTDAQIETGFKLYSPKGCDKCKGGYKGRVGIYEVVRVTPPMQELIMNEASSLELAKQAQKERFRNLRQSALRKVIEGVTS
LEDANRVTKD

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=25204 HCH_RS23705 WP_011399016.1 5419902..5421614(+) (pilB) [Hahella chejuensis KCTC 2396]
ATGAATACTCAAATGACCACTCCCCTTACAGGATTAGCGCGCCGCTTTGTCCAAGACGGAATTATCGAGGAAAATACTGC
GCGCGAAGCGCAGAATAATGCAGGCAAAGCAAAACTCCCTTTTGTGAGCTATCTAGTGCAACAGGGACTGGCCAAAGCCA
CCCAGTTAGCTTACGCCGCCTCAGCAGAATTTGGTGTGCCTTTAGTTGACCTGAACTCTGTTGCTTTAGACCAAATACCC
GAGAAAATTGTAGATGAAAAGCTGATTAGAAAGCATCACGCCTTGCCTCTGTTTAAAAGAGGTAACCGGTTGTTCCTGGC
CCTAGCTGACCCAACAAACCTTCAGGCGCTTGACGAAATAAAGTTCCATACAGGGCTTAGCACGGATGCAGTTCTCGTTG
AGGACGATAAGCTTTCTACCGCTATCGATAGATATCTCGAAGCCCAAGAGCAGCCTCTGGGCGATATAGATGGCGGGGAT
TTAGATGATATCGATATAGAGACCGGCGACGATAGCGACAAGGACGACGATTCAGGAGCCGAACAAGATGACGCCCCTAT
CGTCAAATACATCAACAAGATGCTACTTGACGCAATCAAAGGGGGGGCTTCAGACATACACTTTGAGCCTTACGAAAAAA
CCTATCGCGTCAGATTTAGGACAGATGGCATATTGCATGAGGTATCAAAGCCATCTATAAAACTCGCAGCCAAGCTGTCG
GCGCGTTTGAAAGTAATGTCACAGCTGGACATTTCCGAGAGACGGGTCCCTCAGGATGGCCGTATCAAGATGAAGCTATC
CAAAACCAAAGCCATAGATTTTCGAGTGAACACTCTCCCCACGCTCTGGGGAGAAAAAATCGTTTTACGGATATTAGACC
CAAGTAGCGCGAAACTCGGTATAGACGCCCTTGGGTACGAAGAAGATCAGAAGAAAATGTATCTGGACGCTCTTGCGCAA
CCACAAGGCATGATACTGGTTACCGGGCCTACCGGAAGCGGTAAAACCGTCTCGCTTTATACAGGCCTTAATATTCTGAA
CACTACAGAAAGAAACATATCTACTGCAGAAGACCCGGTGGAAATCAACTTGGAAGGCATTAACCAAGTTAACGTAAACA
CCAAAGTCGGTTTAGACTTTTCTTCGGCTTTGCGAGCATTTCTTCGCCAAGACCCAGACGTTGTTATGGTGGGAGAGATA
CGGGATTTGGATACTGCGTCTATCGCGATTAAAGCGGCTCAAACAGGGCACTTAGTGTTATCAACGCTTCATACGAATAG
CGCCGCAGAAACCTTGACGCGCCTACGTAATATGGGAGTGCCTGCCTTCAATATTGCAACCTCGGTCAGCCTAATCATTG
CACAGCGTCTTGCGAGAAGGCTCTGCGCGGAGTGTAAAGAGGTGATTACCGTCCCCAAAGAAGTTTTAAAGGAGGAAGGA
TTCACAGATGCACAAATTGAAACGGGCTTTAAATTGTACAGCCCTAAAGGTTGTGATAAATGCAAAGGCGGGTATAAAGG
CCGAGTAGGCATATACGAAGTAGTGAGAGTCACGCCCCCTATGCAAGAACTCATTATGAATGAAGCAAGTTCGTTGGAAC
TTGCCAAGCAAGCTCAGAAAGAACGGTTTCGCAATCTCAGACAGTCGGCGCTGAGGAAAGTTATTGAGGGCGTAACCAGC
CTAGAAGACGCAAATCGGGTAACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q2SBM2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.29

100

0.586
  pilB Acinetobacter baylyi ADP1

56.993

100

0.572
  pilB Legionella pneumophila strain ERS1305867

56.437

99.474

0.561
  pilB Vibrio campbellii strain DS40M4

50.977

98.772

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

54.269

92.456

0.502
  pilB Vibrio cholerae strain A1552

52.941

92.456

0.489
  pilF Neisseria gonorrhoeae MS11

47.88

99.298

0.475
  pilB/pilB1 Synechocystis sp. PCC 6803

40.364

96.491

0.389
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.262

94.912

0.363

Multiple sequence alignment