Detailed information NTG0250587

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: CRH11_RS17870
• Genome accession: NZ_CP023859
• Coordinates: 3586777..3587391 (-)
• Length: 204 a.a.
• NCBI ID: WP_043021637.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain L-1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3581777..3592391

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CRH11_RS17845 (CRH11_17840)	rpsT	3582080..3582346 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
CRH11_RS17850 (CRH11_17845)	holA	3582363..3583404 (-)	1042	Protein_3450	DNA polymerase III subunit delta	-
CRH11_RS20720	-	3583444..3583595 (-)	152	Protein_3451	hypothetical protein	-
CRH11_RS17855 (CRH11_17850)	-	3583636..3583770 (+)	135	WP_003152870.1	YqzM family protein	-
CRH11_RS17860 (CRH11_17855)	comEC	3583789..3586140 (-)	2352	WP_094031847.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
CRH11_RS17865 (CRH11_17860)	-	3586141..3586710 (-)	570	WP_094031848.1	ComE operon protein 2	-
CRH11_RS17870 (CRH11_17865)	comEA	3586777..3587391 (-)	615	WP_043021637.1	helix-hairpin-helix domain-containing protein	Machinery gene
CRH11_RS17875 (CRH11_17870)	comER	3587450..3588271 (+)	822	WP_012118027.1	late competence protein ComER	-
CRH11_RS17880 (CRH11_17875)	-	3588340..3589077 (-)	738	WP_076424998.1	class I SAM-dependent DNA methyltransferase	-
CRH11_RS17885 (CRH11_17880)	rsfS	3589074..3589430 (-)	357	WP_007408260.1	ribosome silencing factor	-
CRH11_RS17890 (CRH11_17885)	yqeK	3589448..3590008 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
CRH11_RS17895 (CRH11_17890)	-	3589998..3590567 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
CRH11_RS17900 (CRH11_17895)	yhbY	3590578..3590868 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
CRH11_RS17905 (CRH11_17900)	aroE	3590862..3591704 (-)	843	WP_094031849.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22165.31 Da
• Isoelectric Point: 6.4888

>NTDB_id=250587 CRH11_RS17870 WP_043021637.1 3586777..3587391(-) (comEA) [Bacillus velezensis strain L-1]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=250587 CRH11_RS17870 WP_043021637.1 3586777..3587391(-) (comEA) [Bacillus velezensis strain L-1]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363