Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   CGZ65_RS11805 Genome accession   NZ_CP023429
Coordinates   2358238..2359917 (-) Length   559 a.a.
NCBI ID   WP_096295946.1    Uniprot ID   A0A3N4N3J8
Organism   Neisseria weixii strain 10022     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2353238..2364917
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CGZ65_RS11775 (CGZ65_11775) lspA 2353675..2354174 (+) 500 Protein_2408 signal peptidase II -
  CGZ65_RS11780 (CGZ65_11780) ispH 2354199..2355167 (+) 969 WP_096295941.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  CGZ65_RS11785 (CGZ65_11785) yacG 2355260..2355445 (-) 186 WP_096295942.1 DNA gyrase inhibitor YacG -
  CGZ65_RS11790 (CGZ65_11790) coaE 2355448..2356056 (-) 609 WP_096295943.1 dephospho-CoA kinase -
  CGZ65_RS11795 (CGZ65_11795) pilD 2356057..2356920 (-) 864 WP_096295944.1 prepilin peptidase Machinery gene
  CGZ65_RS11800 (CGZ65_11800) pilG 2356922..2358163 (-) 1242 WP_096295945.1 type II secretion system F family protein Machinery gene
  CGZ65_RS11805 (CGZ65_11805) pilF 2358238..2359917 (-) 1680 WP_096295946.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CGZ65_RS11815 (CGZ65_11815) - 2360586..2361152 (+) 567 WP_096295947.1 hypothetical protein -
  CGZ65_RS11820 (CGZ65_11820) groL 2361472..2363105 (-) 1634 Protein_2416 chaperonin GroEL -
  CGZ65_RS11825 (CGZ65_11825) groES 2363178..2363465 (-) 288 WP_095501906.1 co-chaperone GroES -
  CGZ65_RS11830 (CGZ65_11830) - 2363838..2364835 (+) 998 Protein_2418 siderophore ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62352.84 Da        Isoelectric Point: 6.6200

>NTDB_id=247034 CGZ65_RS11805 WP_096295946.1 2358238..2359917(-) (pilF) [Neisseria weixii strain 10022]
MSVGLLRVLVQSQTITPTQAEHYQAAIKAHKDIMSMLFDDGVISPKALGELVARVFSYPLLDLHHYPRSHVLTDVLTEEQ
MVQNRCVPIFRRGRKVYLAVSDPTQIQNFQKIAFSLGISVDLVVVPHDQLSSLLEWIGQRSTTILKEITQEQESSQPSQA
LYIDNEEAEDGPIPRFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISEK
RVPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDATTLNIDQLGFEPFQKEMLLEAIHRPYGMVLVTGPTGS
GKTVSLYTCLNILNTEDVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCPNCKKAIERPPVPALKKAGFTDEDLAKDWQM
YRPVGCDSCRGKGFKGRVGVYEVMPITEEMQRVIMNNGTEVDIQNMAYQEGMVDLRRAGLLKVMQGLTSLEEVTAHTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=247034 CGZ65_RS11805 WP_096295946.1 2358238..2359917(-) (pilF) [Neisseria weixii strain 10022]
ATGAGTGTCGGATTACTGCGCGTATTGGTGCAAAGTCAAACGATTACGCCTACTCAGGCAGAACATTATCAAGCGGCGAT
TAAAGCGCATAAAGACATTATGTCGATGCTTTTTGATGATGGTGTGATTTCTCCCAAAGCCCTTGGTGAGCTGGTGGCGC
GTGTGTTCAGCTATCCTTTACTGGATTTGCATCATTATCCGCGCAGTCATGTGTTGACGGATGTTTTGACTGAAGAGCAG
ATGGTGCAAAACCGATGTGTGCCGATTTTCCGCCGAGGCCGTAAGGTTTATTTGGCGGTGTCTGATCCGACCCAGATTCA
AAATTTCCAAAAAATCGCTTTTTCTTTAGGTATTTCCGTTGATTTGGTGGTGGTGCCGCATGACCAGTTAAGTTCTTTGC
TGGAATGGATTGGTCAACGCTCGACCACCATTTTGAAAGAAATCACCCAAGAGCAGGAATCTTCCCAACCATCGCAAGCG
CTGTATATCGACAATGAAGAAGCGGAAGACGGCCCGATTCCGCGTTTTATTCATAAAACATTGTCTGATGCCTTGAATGC
CGGCGCATCGGATATCCATTTTGAATTTTACGAACAAATGGCACGCGTGCGTTTCCGTGTTGATGGACAGTTGCGTGAAG
TGGTACAGCCGCCAGTTGCCGTGCGCGGCCAGTTGGCATCGCGTATTAAAGTGATGGCGCGTTTGGATATCTCTGAAAAA
CGTGTACCGCAAGACGGCCGTATTCAGATTGCTTTTCATAAACATGGCCGTCCGATTGACTTCCGTGTGAGCACCTTGCC
GACTTTGTTCGGTGAAAAAGTGGTAATGCGTATCCTGAATTCGGATGCCACTACATTGAATATCGACCAATTGGGCTTTG
AGCCATTCCAAAAAGAAATGCTGCTTGAAGCTATTCATCGCCCTTACGGTATGGTGCTGGTGACCGGCCCTACCGGCTCG
GGTAAAACTGTTTCGCTTTATACCTGTTTAAATATTCTAAATACCGAAGATGTGAATATTTCCACGGCAGAAGACCCTGC
CGAGATCAACTTGCCGGGGATTAATCAGGTCAACGTCAATGACAAACAGGGTCTGACCTTTGCGGCCGCGCTGAAATCTT
TCCTGCGTCAGGATCCGGATATCATTATGGTCGGTGAGATTCGTGACTTGGAAACTGCAGATATTGCGATTAAAGCGGCG
CAAACCGGCCATATGGTGTTCTCGACATTGCATACCAATAATGCACCGGCAACTTTGTCGCGTATGTTGAATATGGGGGT
GGCGCCATTTAATATCGCCAGCTCGGTGAGTTTGATTATGGCGCAGCGTTTGTTGCGTCGCTTGTGTCCAAACTGCAAAA
AAGCGATTGAGCGCCCGCCGGTGCCGGCATTGAAAAAAGCCGGTTTTACCGATGAAGATTTGGCCAAAGATTGGCAAATG
TACCGCCCTGTCGGTTGCGACAGTTGTCGTGGCAAGGGTTTCAAAGGTCGTGTCGGCGTGTATGAAGTAATGCCGATTAC
CGAAGAAATGCAGCGTGTCATTATGAATAACGGTACGGAAGTCGATATTCAAAACATGGCTTATCAAGAAGGTATGGTGG
ATTTGCGCCGTGCAGGTCTATTGAAAGTGATGCAGGGTTTAACTTCGTTGGAAGAAGTAACGGCTCACACCAATGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(179-447)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3N4N3J8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

79.249

100

0.792
  pilB Acinetobacter baylyi ADP1

50.699

100

0.519
  pilB Acinetobacter baumannii D1279779

51.068

100

0.513
  pilB Legionella pneumophila strain ERS1305867

46.996

100

0.476
  pilB Vibrio parahaemolyticus RIMD 2210633

45.213

100

0.456
  pilB Vibrio cholerae strain A1552

45.018

100

0.453
  pilB Vibrio campbellii strain DS40M4

43.617

100

0.44
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.623

93.56

0.361

Multiple sequence alignment