Detailed information NTG0246864

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: CMR26_RS04110
• Genome accession: NZ_CP023414
• Coordinates: 790656..791270 (+)
• Length: 204 a.a.
• NCBI ID: WP_108724811.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain BS-37
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 785656..796270

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CMR26_RS04075 (CMR26_04070)	aroE	786344..787186 (+)	843	WP_059368084.1	shikimate dehydrogenase	-
CMR26_RS04080 (CMR26_04075)	yhbY	787180..787470 (+)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
CMR26_RS04085 (CMR26_04080)	-	787481..788050 (+)	570	WP_042635364.1	nicotinate-nucleotide adenylyltransferase	-
CMR26_RS04090 (CMR26_04085)	yqeK	788040..788600 (+)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
CMR26_RS04095 (CMR26_04090)	rsfS	788617..788973 (+)	357	WP_007408260.1	ribosome silencing factor	-
CMR26_RS04100 (CMR26_04095)	-	788970..789707 (+)	738	WP_015240247.1	class I SAM-dependent DNA methyltransferase	-
CMR26_RS04105 (CMR26_04100)	comER	789776..790597 (-)	822	WP_053103971.1	late competence protein ComER	-
CMR26_RS04110 (CMR26_04105)	comEA	790656..791270 (+)	615	WP_108724811.1	helix-hairpin-helix domain-containing protein	Machinery gene
CMR26_RS04115 (CMR26_04110)	-	791337..791906 (+)	570	WP_003152868.1	ComE operon protein 2	-
CMR26_RS04120 (CMR26_04115)	comEC	791907..794258 (+)	2352	WP_108724812.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
CMR26_RS04125 (CMR26_04120)	-	794277..794411 (-)	135	WP_003152870.1	YqzM family protein	-
CMR26_RS20050	-	794452..794608 (+)	157	Protein_786	hypothetical protein	-
CMR26_RS04130 (CMR26_04125)	holA	794643..795684 (+)	1042	Protein_787	DNA polymerase III subunit delta	-
CMR26_RS04135 (CMR26_04130)	rpsT	795701..795967 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22137.25 Da
• Isoelectric Point: 5.5107

>NTDB_id=246864 CMR26_RS04110 WP_108724811.1 790656..791270(+) (comEA) [Bacillus velezensis strain BS-37]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGQFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=246864 CMR26_RS04110 WP_108724811.1 790656..791270(+) (comEA) [Bacillus velezensis strain BS-37]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCTGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACAGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363