Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CLM74_RS16880 Genome accession   NZ_CP023271
Coordinates   3686489..3688219 (-) Length   576 a.a.
NCBI ID   WP_105164558.1    Uniprot ID   A0AA43HMP5
Organism   Stenotrophomonas sp. MYb57     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3681489..3693219
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLM74_RS16865 (CLM74_16795) sucC 3681603..3682772 (-) 1170 WP_025874298.1 ADP-forming succinate--CoA ligase subunit beta -
  CLM74_RS16870 (CLM74_16800) - 3683149..3684762 (+) 1614 WP_105164557.1 ATP-binding protein -
  CLM74_RS16875 (CLM74_16805) pilR 3684817..3686190 (+) 1374 WP_025874296.1 sigma-54 dependent transcriptional regulator Regulator
  CLM74_RS16880 (CLM74_16810) pilB 3686489..3688219 (-) 1731 WP_105164558.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CLM74_RS21815 (CLM74_16815) - 3688349..3688729 (-) 381 WP_105164559.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CLM74_RS16890 (CLM74_16820) pilC 3689077..3690336 (+) 1260 WP_105164560.1 type II secretion system F family protein Machinery gene
  CLM74_RS16895 (CLM74_16825) - 3690344..3691207 (+) 864 WP_025874291.1 A24 family peptidase -
  CLM74_RS16900 (CLM74_16830) coaE 3691219..3691830 (+) 612 WP_105164561.1 dephospho-CoA kinase -
  CLM74_RS16905 (CLM74_16835) - 3691883..3692269 (-) 387 WP_105164562.1 hypothetical protein -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62257.20 Da        Isoelectric Point: 5.8712

>NTDB_id=245656 CLM74_RS16880 WP_105164558.1 3686489..3688219(-) (pilB) [Stenotrophomonas sp. MYb57]
MNAVTSANLVGITGLARRLVQDGALDEAGARDAMAKATAARQPLPTWFAQNKVVSPPQLAAANAVEFGMPLFDVSVFDAS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWHSSHDTLGDSLGGD
DDGLANLDISGGDDDMAGGTDSGIDAKGDDTPVVKFINKVLIDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPNK
LNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLF
LEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDI
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCSNCKRPADLPAH
ALLAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGLYQVMPMTDEIATIVLAGGNALQIAEAAQAIGVNDLRQSALKKA
AAGVTSLAEINRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=245656 CLM74_RS16880 WP_105164558.1 3686489..3688219(-) (pilB) [Stenotrophomonas sp. MYb57]
ATGAACGCAGTCACCTCCGCCAATCTCGTCGGCATCACCGGTCTGGCCCGTCGTCTGGTCCAGGACGGCGCACTGGATGA
AGCCGGTGCCCGCGATGCCATGGCCAAGGCCACCGCAGCCCGCCAGCCGCTGCCCACCTGGTTCGCGCAGAACAAGGTGG
TCTCACCGCCGCAGCTGGCCGCTGCCAATGCGGTCGAGTTCGGCATGCCGCTGTTCGACGTGTCGGTATTCGATGCCAGC
CAGAACGCGATGAGCCTGGTCAGCGAGGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAGCGTGGCGGCAAGCT
GTTCGTCGGCACCAGCAACCCGACTCATTCACTGGACGAAATCAAGTTCCACACCAATCTGGTGGTGGAGCCGATCCTGG
TTGACGAGGACCAGATCCGCCGCACTCTGGAGCAGTGGCACTCCAGCCATGACACGCTGGGCGATTCGCTGGGCGGAGAT
GACGATGGTCTCGCCAACCTTGATATCTCCGGTGGCGACGATGACATGGCCGGCGGCACCGATTCGGGCATCGACGCCAA
GGGCGACGATACTCCGGTGGTGAAGTTCATCAACAAGGTGTTGATCGATGCGATCCGAAAGGGTGCCTCGGACATCCACT
TCGAGCCCTACGAAGACGATTACCGCGTGCGCCTGCGCATCGATGGCCTGTTGAAAATGGTCGCGCGTGCGCCGAACAAG
CTCAACCAGCGCATTGCCGCGCGCCTGAAGGTGATGGCGCAGCTGGATATCGCTGAAAAGCGCGTGCCGCAGGATGGCCG
CATCAAGCTCAATCTTTCCAAGACCAAGCAGATCGACTTTCGTGTCAGCACCCTGCCGACGCTGTTCGGCGAGAAGGTGG
TGCTGCGTATCCTCGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCGGACCAGCAGAAGCTGTTC
CTGGAGGCGATCCACAAGCCTTACGGCATGGTACTGGTGACCGGCCCGACCGGTTCAGGCAAGACGGTGTCCCTGTATAC
CGCACTGGGCATCCTCAACGACGAGACCCGCAACATCTCCACCGCCGAGGATCCGGTGGAAATCCGCCTACCCGGCGTCA
ACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTTGCCGCAGCGCTGCGCTCGTTCCTGCGACAGGATCCGGACATC
ATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCGGAAATTGCGATCAAGGCTGCGCAGACCGGTCACATGGTGCTGTC
CACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCGTACAACATCACCAGCT
CGGTGACGCTGGTGATCGCCCAGCGCCTGGCGCGCCGCCTGTGTTCGAACTGCAAGCGGCCGGCCGACCTGCCTGCACAC
GCCCTTCTGGCCGAGGGCTTCACCCAGGCGCAGCTGGATGCAGGCATCGAGCTGTATGAGGCGGTAGGCTGCGACGAATG
CACCGAGGGCTACAAGGGCCGTACCGGCCTCTACCAGGTGATGCCGATGACCGACGAGATCGCGACCATCGTGCTGGCGG
GCGGCAATGCGCTGCAGATTGCCGAGGCCGCGCAGGCGATCGGTGTGAATGACCTGCGGCAGTCGGCGTTGAAGAAGGCG
GCCGCGGGTGTGACCAGCCTGGCCGAGATCAATCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-149)

Type II/IV secretion system protein (T2SSE)

(199-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.866

98.611

0.561
  pilB Acinetobacter baylyi ADP1

56.021

99.479

0.557
  pilB Legionella pneumophila strain ERS1305867

52.197

98.785

0.516
  pilF Neisseria gonorrhoeae MS11

49.736

98.785

0.491
  pilB Vibrio cholerae strain A1552

52.107

90.625

0.472
  pilB Vibrio parahaemolyticus RIMD 2210633

45.66

100

0.457
  pilB Vibrio campbellii strain DS40M4

45.201

99.479

0.45

Multiple sequence alignment