Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PSYC_RS00330 Genome accession   NC_007204
Coordinates   70862..72571 (-) Length   569 a.a.
NCBI ID   WP_041757403.1    Uniprot ID   -
Organism   Psychrobacter arcticus 273-4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 65862..77571
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSYC_RS00305 (Psyc_0059) - 66427..66762 (+) 336 WP_011279372.1 pyrimidine/purine nucleoside phosphorylase -
  PSYC_RS00310 (Psyc_0060) rlmB 66968..67738 (+) 771 WP_011279373.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  PSYC_RS00315 (Psyc_0061) coaE 67701..68393 (-) 693 WP_011279374.1 dephospho-CoA kinase -
  PSYC_RS00320 (Psyc_0062) pilD 68477..69376 (-) 900 WP_011279375.1 prepilin peptidase Machinery gene
  PSYC_RS00325 (Psyc_0063) pilC 69402..70625 (-) 1224 WP_011279376.1 type II secretion system F family protein Machinery gene
  PSYC_RS00330 (Psyc_0064) pilB 70862..72571 (-) 1710 WP_041757403.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSYC_RS00335 (Psyc_0065) tpiA 73127..73936 (+) 810 WP_011279378.1 triose-phosphate isomerase -
  PSYC_RS00340 (Psyc_0066) secG 74165..74473 (+) 309 WP_011279379.1 preprotein translocase subunit SecG -
  PSYC_RS00355 (Psyc_0067) rimP 75377..75877 (+) 501 WP_011279380.1 ribosome maturation factor RimP -
  PSYC_RS00360 (Psyc_0068) nusA 76010..77494 (+) 1485 WP_011279381.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62786.22 Da        Isoelectric Point: 5.2185

>NTDB_id=24427 PSYC_RS00330 WP_041757403.1 70862..72571(-) (pilB) [Psychrobacter arcticus 273-4]
MSITTSKYGGLAQQLISEGIVSEAHMKAAQTDSQQQQIGLVSYLVEHKLANAYQLAQMLSQAFGDPLFDLDALSSEVIPK
DLVDEKIVRKFNALPMFKRGQRLFVALSDPTRIDAIDAIAFNSRLSIETVVVEEDKLKRCIENLYADTMQSFDSFSDSDL
NIGFDESEEEDSATKLSDSVDEAPVVKFVNKMLVDAIRMGASDLHFEPYEKSYRVRFRVDGVMQKMANPPVQLAGKIAAR
LKVMSQMDISERRVPQDGRMKLKISKDKAIDFRVSCLPTLFGEKLVLRILDPSSAMLGIEGLGYEPDQQDMFLEALHKPQ
GMLLITGPTGSGKTVSLYTGINILNTGSTNISTAEDPVEINLEGINQVNVNPKVGLTFSTALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLLNMGVASFNIATSVNLVIAQRLARRLCKSCKKSINIPRQSLLELGFT
DADLDNTDNIIYEPVGCNKCREGYKGRVGIYEVMKITPDISRIIMEDGNAIDIKDAALKNGFRDLRRSGVLKVLQGVTSI
QEMMRVTSG

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=24427 PSYC_RS00330 WP_041757403.1 70862..72571(-) (pilB) [Psychrobacter arcticus 273-4]
ATGTCTATCACAACCAGTAAATACGGTGGCTTAGCACAGCAGCTGATTAGCGAAGGTATCGTAAGCGAAGCGCATATGAA
AGCGGCGCAGACTGATTCGCAGCAGCAGCAAATAGGACTGGTCTCTTATCTGGTAGAGCACAAGCTTGCCAATGCCTATC
AATTGGCACAAATGCTGTCGCAAGCGTTTGGCGACCCTTTATTTGATCTCGATGCGCTCAGCTCAGAGGTTATCCCAAAA
GATTTGGTCGATGAAAAAATCGTGCGTAAGTTTAATGCCTTACCAATGTTTAAGCGCGGGCAACGCTTGTTTGTGGCACT
TAGTGATCCGACCCGTATCGATGCGATTGATGCGATTGCCTTCAACTCACGCTTGTCGATTGAAACAGTCGTGGTTGAAG
AAGACAAGCTTAAAAGGTGTATTGAAAACCTTTACGCTGATACCATGCAAAGTTTTGATAGCTTTTCTGATAGTGATTTA
AATATTGGCTTTGATGAGAGTGAAGAAGAGGACAGCGCGACCAAACTTTCTGATAGTGTCGATGAAGCACCGGTCGTCAA
GTTTGTTAATAAAATGCTGGTTGATGCCATTCGTATGGGTGCATCCGACTTACATTTTGAGCCCTATGAAAAAAGCTATC
GAGTGCGCTTTCGAGTTGACGGTGTGATGCAAAAGATGGCCAATCCGCCCGTGCAGCTTGCTGGAAAAATTGCCGCACGT
CTAAAAGTGATGTCACAAATGGACATCTCAGAGCGCCGTGTACCACAAGATGGGCGTATGAAGCTTAAGATATCTAAAGA
TAAGGCCATCGACTTTCGGGTAAGCTGCCTGCCAACCTTATTTGGCGAAAAGCTTGTTTTGCGTATCTTAGACCCTTCAT
CAGCCATGCTCGGTATTGAAGGATTAGGCTACGAGCCTGACCAACAAGATATGTTTTTAGAAGCGTTACATAAACCGCAA
GGGATGCTTCTTATCACAGGTCCTACTGGCTCTGGTAAAACCGTCTCACTTTATACTGGCATCAATATTCTGAATACTGG
TTCGACCAATATCTCAACCGCCGAAGACCCTGTCGAAATTAACCTTGAAGGCATTAATCAGGTCAACGTCAACCCAAAAG
TGGGATTGACCTTCTCTACTGCCCTCAAATCATTTTTACGCCAAGACCCTGATATCGTGATGGTTGGTGAAATTCGTGAC
CTTGAAACCGCTGAAATTGCGATTAAAGCAGCACAAACAGGGCATATGGTACTTTCAACCCTACATACCAACTCAGCGCC
TGAAACATTGACGCGGTTACTCAACATGGGTGTAGCTTCCTTTAATATTGCTACTTCCGTCAACTTGGTCATTGCCCAAC
GGTTAGCACGGCGCTTATGTAAAAGCTGTAAAAAATCCATCAATATTCCGCGTCAAAGTTTGCTTGAGCTGGGCTTTACC
GATGCTGACTTGGACAATACAGACAATATCATTTATGAGCCGGTCGGTTGTAATAAATGCCGTGAAGGCTATAAAGGGCG
TGTCGGTATTTATGAGGTTATGAAAATCACTCCCGACATTTCACGTATTATTATGGAAGATGGTAACGCGATAGATATCA
AAGATGCGGCACTCAAAAATGGTTTTAGAGACTTACGTCGTTCTGGTGTTTTAAAGGTGTTACAAGGCGTGACCAGTATT
CAAGAAATGATGCGCGTGACGTCTGGATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

67.018

100

0.671
  pilB Acinetobacter baylyi ADP1

63.781

99.473

0.634
  pilB Legionella pneumophila strain ERS1305867

51.646

100

0.524
  pilF Neisseria gonorrhoeae MS11

50.984

98.243

0.501
  pilB Vibrio cholerae strain A1552

50.28

94.025

0.473
  pilB Vibrio parahaemolyticus RIMD 2210633

48.983

95.079

0.466
  pilB Vibrio campbellii strain DS40M4

48.141

94.552

0.455
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.288

100

0.381
  pilB/pilB1 Synechocystis sp. PCC 6803

37.205

96.837

0.36

Multiple sequence alignment