Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CJ010_RS19950 Genome accession   NZ_CP022958
Coordinates   4325834..4327549 (+) Length   571 a.a.
NCBI ID   WP_141019671.1    Uniprot ID   -
Organism   Azoarcus sp. DD4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4320834..4332549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CJ010_RS19925 (CJ010_19870) - 4321205..4321990 (-) 786 WP_141019667.1 M48 family metallopeptidase -
  CJ010_RS19930 (CJ010_19875) - 4321972..4322721 (-) 750 WP_141019668.1 molybdopterin-binding protein -
  CJ010_RS19935 (CJ010_19880) - 4322718..4323500 (-) 783 WP_141019669.1 EI24 domain-containing protein -
  CJ010_RS19940 (CJ010_19885) ffh 4323503..4324861 (-) 1359 WP_141019670.1 signal recognition particle protein -
  CJ010_RS19945 (CJ010_19890) - 4324913..4325740 (+) 828 WP_141020791.1 inner membrane protein YpjD -
  CJ010_RS19950 (CJ010_19895) pilB 4325834..4327549 (+) 1716 WP_141019671.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CJ010_RS19955 (CJ010_19900) pilC 4327573..4328808 (+) 1236 WP_141019672.1 type II secretion system F family protein Machinery gene
  CJ010_RS19960 (CJ010_19905) pilD 4328855..4329706 (+) 852 WP_141019673.1 A24 family peptidase Machinery gene
  CJ010_RS19965 (CJ010_19910) - 4329863..4330123 (+) 261 WP_141019674.1 FmdB family zinc ribbon protein -
  CJ010_RS19970 (CJ010_19915) - 4330136..4330762 (+) 627 WP_141019675.1 DUF502 domain-containing protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62139.43 Da        Isoelectric Point: 5.3344

>NTDB_id=243722 CJ010_RS19950 WP_141019671.1 4325834..4327549(+) (pilB) [Azoarcus sp. DD4]
MAANPQTALSGLARALIQHGRLSEADVLSCSPKSTGSSNAFLLEIAQRGLMSTRDIARFAAETFGYPLLDIGAIDKASIP
RDAVDRKLMGKHQVVALGKRQNRLTLAVADPSNMRVLDEIRFQTGLQVDLIVVEVDKLCKLADTLAESTEQTLKDLTGEE
FDMDLLQQDTAGEQVSEDSSAEVDDAPVVKFIQKVLIDAINEGASDIHFEPYEKYYRIRVRTDGILREIAQPPLVLKEKI
AARIKVISRLDISEKRIPQDGRMKLVLSKNKAIDFRVSTLPTLHGEKIVMRILDPSSAMLGVDALGYEPEQKKALLDAVE
RPYGMILVTGPTGSGKTVSLYTCLNLLNKAGVNISTAEDPAEINLPGINQVNVNEKAGLTFAASLRAFLRQDPDVIMVGE
IRDLETAEISVKAAQTGHLVLSTLHTNDAPTTLERLKNMGVAPFNIASSVIMITAQRLARRLCSCKKPVDIPIEALLQAG
FSEEDLDGSWQPYGPVGCDRCKGSGYKGRVGIYQVMPVTEEIAHIIMTNGNSMDIAAQAKREGVSDLRQSGLLKVKQGVT
SLEEVLASTNE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=243722 CJ010_RS19950 WP_141019671.1 4325834..4327549(+) (pilB) [Azoarcus sp. DD4]
ATGGCAGCAAACCCCCAGACCGCACTGAGCGGGCTTGCCCGAGCACTGATCCAGCATGGTCGGCTGAGTGAGGCCGACGT
CCTGTCCTGTTCACCCAAAAGTACTGGATCAAGCAACGCCTTCCTGCTCGAGATCGCACAACGCGGTCTGATGAGCACAC
GCGACATCGCACGCTTCGCAGCCGAAACGTTCGGCTACCCGCTGCTGGACATCGGCGCCATCGACAAAGCCTCGATCCCG
CGCGACGCGGTCGACCGCAAGCTGATGGGCAAGCATCAGGTGGTCGCGCTCGGCAAGCGCCAGAACCGCCTCACCCTTGC
CGTCGCAGACCCATCGAACATGCGGGTGCTCGACGAAATCCGCTTCCAGACCGGGCTGCAGGTCGATCTCATCGTGGTGG
AGGTCGACAAGTTGTGCAAGCTTGCCGACACCCTTGCCGAGTCGACCGAGCAGACGCTCAAGGATCTGACCGGCGAAGAG
TTCGACATGGATCTCCTGCAGCAGGACACTGCGGGAGAACAGGTCAGCGAGGACAGCAGCGCAGAGGTCGACGACGCCCC
TGTCGTCAAGTTCATCCAGAAGGTACTGATAGACGCGATCAACGAAGGCGCGTCCGATATCCACTTCGAGCCCTACGAAA
AGTACTACCGGATCCGCGTGCGCACCGACGGCATACTGCGCGAGATCGCGCAGCCGCCACTGGTCCTGAAGGAAAAGATC
GCCGCCCGCATCAAGGTCATCTCGCGCCTGGACATCTCGGAAAAACGCATTCCGCAGGACGGCCGGATGAAACTGGTCCT
GTCCAAGAACAAGGCCATCGACTTCCGCGTTTCCACACTGCCGACCTTGCACGGCGAGAAGATCGTGATGCGTATTCTCG
ATCCTAGCTCTGCCATGCTCGGTGTCGATGCCCTCGGCTACGAGCCCGAGCAGAAAAAGGCCCTGCTCGACGCCGTCGAA
CGCCCATACGGCATGATCCTCGTCACCGGCCCGACCGGGTCGGGCAAAACTGTATCGCTCTACACCTGCCTCAACCTGCT
CAACAAGGCGGGCGTAAACATCTCCACCGCCGAGGATCCGGCCGAAATCAATTTGCCCGGCATCAACCAGGTCAACGTCA
ATGAAAAGGCCGGCCTCACCTTCGCCGCGTCGCTGCGCGCCTTCCTGCGCCAGGATCCGGACGTGATCATGGTCGGCGAA
ATCCGCGACCTGGAAACGGCCGAGATATCGGTCAAGGCAGCGCAGACCGGCCACCTGGTGCTATCCACGCTGCACACCAA
TGACGCCCCCACCACGCTGGAACGCCTGAAGAACATGGGGGTCGCCCCGTTCAACATCGCCTCGTCGGTCATCATGATCA
CTGCCCAACGGCTGGCCCGGCGCCTGTGCTCGTGCAAGAAGCCGGTGGACATCCCGATCGAGGCACTGTTGCAGGCCGGT
TTCAGCGAAGAAGACCTCGACGGCAGTTGGCAACCCTATGGCCCGGTCGGCTGCGATCGTTGCAAGGGCAGCGGCTACAA
GGGACGTGTAGGCATCTATCAGGTCATGCCGGTCACTGAAGAGATTGCACATATCATCATGACCAACGGCAACTCGATGG
ACATCGCAGCCCAAGCCAAGCGCGAGGGCGTCAGCGACCTGCGCCAGTCCGGCCTGCTGAAGGTCAAGCAGGGAGTCACT
TCGCTGGAAGAAGTCCTCGCCTCCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.712

99.65

0.555
  pilB Acinetobacter baylyi ADP1

55.124

99.124

0.546
  pilB Legionella pneumophila strain ERS1305867

53.616

99.299

0.532
  pilF Neisseria gonorrhoeae MS11

55.133

92.119

0.508
  pilB Vibrio cholerae strain A1552

50.943

92.82

0.473
  pilB Vibrio parahaemolyticus RIMD 2210633

48.659

91.419

0.445
  pilB Vibrio campbellii strain DS40M4

47.893

91.419

0.438
  pilF Thermus thermophilus HB27

39.706

95.271

0.378
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.925

92.995

0.371

Multiple sequence alignment