Detailed information NTG0024338

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PSYR_RS15075
• Genome accession: NC_007005
• Coordinates: 3485827..3486996 (-)
• Length: 389 a.a.
• NCBI ID: WP_003400837.1
• Uniprot ID: -
• Organism: Pseudomonas syringae pv. syringae B728a
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3480827..3491996

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSYR_RS15060 (Psyr_2903)	-	3482113..3483177 (+)	1065	WP_003400842.1	microcin C ABC transporter permease YejB	-
PSYR_RS15065 (Psyr_2904)	-	3483177..3484199 (+)	1023	WP_003400841.1	ABC transporter permease	-
PSYR_RS15070 (Psyr_2905)	-	3484201..3485778 (+)	1578	WP_003400838.1	ABC transporter ATP-binding protein	-
PSYR_RS15075 (Psyr_2906)	pilU	3485827..3486996 (-)	1170	WP_003400837.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PSYR_RS15080 (Psyr_2907)	-	3487120..3487395 (+)	276	WP_003400836.1	peptidylprolyl isomerase	-
PSYR_RS15085 (Psyr_2908)	pgm	3487470..3489116 (-)	1647	WP_011268077.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
PSYR_RS15090 (Psyr_2909)	-	3489280..3491607 (-)	2328	WP_011268078.1	ATP-dependent DNA helicase	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42577.65 Da
• Isoelectric Point: 6.0641

>NTDB_id=24338 PSYR_RS15075 WP_003400837.1 3485827..3486996(-) (pilU) [Pseudomonas syringae pv. syringae B728a]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETFKPGEVAVIAQGLMDEEQKLEFQRELEMNLAVSMAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKRGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDSAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGAGSLTTPPPAPTGSSTASTAEWGLVDDDAPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=24338 PSYR_RS15075 WP_003400837.1 3485827..3486996(-) (pilU) [Pseudomonas syringae pv. syringae B728a]
ATGGATTTCCCGGCGCTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTGTACCTCTCCACCGGAGCGCCACCGTG
CGCCAAGTTCAACGGTGTGCTCAAGCCACTGGGCAGCGAAACCTTCAAGCCGGGGGAGGTCGCAGTCATTGCCCAGGGGC
TGATGGACGAAGAACAGAAGCTCGAGTTTCAACGCGAACTGGAGATGAACCTCGCCGTTTCGATGGCGGGCATCGGCCGG
TTCCGGATCAATATCTTTATGCAGCGCAACGAAGTGTCCATCGTCGCGCGGAATATCAAACTGGACATCCCGCGTTTCGA
AGACCTGTTCCTGCCGCCCGTGCTGCTCGACGTGATCATGGAAAAGCGCGGTCTGGTGCTGTTTGTCGGGGCCACGGGTT
CGGGCAAGTCGACCTCATTGGCGGCGCTGATCGACTACCGTAACCGCAACGCCAGCGGCCATATCATCACCATTGAGGAC
CCGGTCGAGTTCATCCATCGCCACAAGAAATCGATCGTCAACCAGCGCGAAGTCGGGGTCGATACCCGCAGCTTCCATGC
GGCATTGAAAAACACGCTGCGCCAGGCGCCGGATGTGATTCTGATCGGCGAGATTCGTGATCGCGAGACCATGGAGCACG
CGCTGGCGTTTGCCGACACCGGCCACCTGGCGATCTCGACCCTGCACGCCAACAACGCCAATCAGGCGCTGGACCGGATC
ATCAATTTCTTCCCGGAAGAGCGTCGCGCGCAGTTGCTGCATGACCTGGGCAACAACCTCAAGGCATTCGTCTCCCAGCG
ACTGGTCAGAACACCGGATGGCAAGCGCCGCGCGGCGGTGGAAGTGATGATGGGCACACCGACCATTCGCGACCTGATCC
AGCGCAATGAACTGACCGAACTCAAGGGCATCATGGAGAAGTCCGGCAGCCTCGGTATGCAGACGTTCGACTCTGCGCTA
TTCAATCTGGCCGTCGAAGGGGCGATCAGCGAAGAAGAAGCCCTCAAGAACGCCGACTCGCAGAACAACGTGCGCCTGCG
CCTCAAACTGCACAGTGAAGGCGGCGCCGGGAGCCTCACCACACCGCCGCCGGCCCCGACCGGCAGCAGCACGGCAAGCA
CCGCAGAGTGGGGGCTGGTCGATGATGACGCGCCGGGTCCGCAGGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.287	93.059	0.542
pilU	Vibrio cholerae strain A1552	51.105	93.059	0.476
pilU	Acinetobacter baylyi ADP1	51.541	91.774	0.473
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.164	98.458	0.386
pilT	Pseudomonas stutzeri DSM 10701	41.108	88.175	0.362
pilT	Pseudomonas aeruginosa PAK	41.108	88.175	0.362