Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VF_RS11380 Genome accession   NC_006840
Coordinates   2456927..2458615 (+) Length   562 a.a.
NCBI ID   WP_011262623.1    Uniprot ID   Q5E2R5
Organism   Aliivibrio fischeri ES114     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2451927..2463615
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VF_RS11360 (VF2182) pdhR 2453565..2454335 (-) 771 WP_005420885.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  VF_RS11365 (VF2183) ampD 2454622..2455173 (-) 552 WP_011262620.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VF_RS11370 (VF2184) nadC 2455287..2456174 (+) 888 WP_005420889.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VF_RS11375 (VF2185) pilA 2456516..2456920 (+) 405 WP_011262622.1 pilin Machinery gene
  VF_RS11380 (VF2186) pilB 2456927..2458615 (+) 1689 WP_011262623.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VF_RS11385 (VF2187) pilC 2458635..2459873 (+) 1239 WP_011262624.1 type II secretion system F family protein Machinery gene
  VF_RS11390 (VF2188) pilD 2459978..2460865 (+) 888 WP_011262625.1 prepilin peptidase Machinery gene
  VF_RS11395 (VF2189) coaE 2460862..2461484 (+) 623 Protein_2204 dephospho-CoA kinase -
  VF_RS11400 (VF2190) zapD 2461490..2462236 (+) 747 WP_005420900.1 cell division protein ZapD -
  VF_RS11405 (VF2191) yacG 2462244..2462453 (+) 210 WP_011262627.1 DNA gyrase inhibitor YacG -
  VF_RS11410 (VF2192) mutT 2462523..2462924 (-) 402 WP_011262628.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62244.87 Da        Isoelectric Point: 5.3872

>NTDB_id=24263 VF_RS11380 WP_011262623.1 2456927..2458615(+) (pilB) [Aliivibrio fischeri ES114]
MHTNLASILRQAELISATQESSIIESVQAETHTVPSALLAQGIFTPDELISHLAQIFGLDVINVTQFDYATTCQKLGLRD
LITRYQALPVSHDSCTIRLAVADPSIAQIEDDFRFTSGKQIELVLADQGQIQAAIRRVYGAGLSANDNQHKEITQDELAS
LVDVSLDEIRATEDLSQDDAPVTRFINQILLEAVRKGVSDIHFEPYEDNYRVRFRCDGILVENSRPSAHLSRRLSARLKI
MAKLDIAERRLPQDGRIKLKLNSTTSIDMRVSSLPTLWGEKIVLRLLDSSAANLNIDKLGYSDQQKALYLEALKRPQGMI
LMTGPTGSGKTVSLYSGLNILNTTEQNISTAEDPVEINLNGINQVQVNPKIGLTFAHALRSFLRQDPDIVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVVRLGNMGIENFNLASSLSLIIAQRLARRLCNLCKQEDHLSDELREEYNIAPSA
IIYQANKEGCNECTHGYSGRIGIYEVMPFTRELSVAILKGATVRQIEQIAIQQGMDNLKTSGIKRLEDGTTSLQELNRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=24263 VF_RS11380 WP_011262623.1 2456927..2458615(+) (pilB) [Aliivibrio fischeri ES114]
ATGCACACCAACCTCGCCTCTATCTTGCGTCAAGCTGAGTTAATCAGTGCGACGCAAGAAAGCAGTATCATAGAGTCAGT
TCAAGCAGAAACGCACACTGTGCCTTCTGCTCTTTTAGCTCAGGGGATATTTACTCCTGATGAACTCATAAGTCACCTTG
CTCAAATCTTTGGCCTTGATGTGATCAACGTGACTCAATTTGATTATGCCACCACTTGTCAAAAGCTGGGACTGCGCGAT
CTCATTACTCGCTATCAAGCTCTTCCTGTAAGTCACGACAGTTGCACGATTCGTCTTGCTGTAGCTGATCCAAGTATTGC
TCAAATAGAAGATGATTTTCGTTTTACTAGCGGAAAACAAATTGAATTAGTACTTGCAGATCAAGGACAAATCCAAGCAG
CCATTCGACGCGTCTACGGTGCAGGATTATCTGCAAATGACAATCAACATAAAGAGATAACCCAAGATGAGTTAGCTTCA
TTAGTGGATGTCTCACTTGATGAGATAAGAGCTACAGAAGATCTCAGCCAAGACGATGCCCCCGTCACTCGCTTTATTAA
CCAAATTTTATTAGAAGCGGTGCGTAAAGGAGTATCAGATATTCATTTTGAACCCTACGAAGATAATTATCGTGTGCGTT
TTCGCTGCGATGGGATCTTAGTTGAAAACAGCCGTCCATCCGCTCACTTAAGTCGTCGTTTATCTGCTCGCCTTAAAATC
ATGGCAAAATTAGACATTGCAGAACGTCGTCTACCACAAGATGGGCGTATTAAACTCAAATTAAACAGTACCACTTCGAT
TGATATGCGAGTTTCTTCACTGCCAACTTTATGGGGTGAGAAAATCGTACTGCGTTTATTAGACAGCAGTGCTGCTAATT
TAAATATTGATAAACTTGGCTATAGCGATCAACAAAAAGCCCTATATTTAGAAGCCCTAAAACGCCCTCAAGGCATGATA
TTAATGACAGGCCCTACAGGCAGTGGGAAAACCGTCTCTCTTTATTCTGGTCTTAATATTCTCAACACCACGGAACAAAA
CATTTCAACGGCGGAAGATCCAGTTGAGATCAACTTAAATGGGATCAACCAAGTTCAAGTTAACCCTAAAATCGGCCTTA
CTTTCGCTCACGCTTTACGATCTTTTTTACGCCAAGATCCAGATATCGTCATGGTAGGTGAAATACGAGATCTCGAAACC
GCTGAGATTGCTGTCAAAGCAGCTCAAACCGGGCACTTAGTTCTTTCTACCCTACATACAAATTCCGCAGCAGAAACCGT
CGTTCGACTAGGCAACATGGGGATTGAAAACTTTAACTTAGCTTCCTCTCTTAGTTTGATTATTGCGCAACGGCTGGCTC
GTCGTCTCTGCAATTTATGCAAGCAAGAAGATCATTTAAGTGATGAGTTGCGAGAAGAATATAACATTGCACCTTCAGCC
ATTATTTATCAAGCCAATAAAGAAGGTTGCAATGAGTGCACTCATGGTTACTCAGGGCGTATTGGTATTTATGAAGTGAT
GCCGTTCACCCGAGAACTGTCTGTTGCCATTTTAAAAGGTGCAACCGTTAGACAAATAGAACAAATCGCCATTCAACAAG
GTATGGATAACCTAAAAACCTCAGGAATTAAACGATTAGAAGATGGCACCACCAGCTTACAAGAACTAAACCGTGTGCTC
TATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5E2R5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

68.327

100

0.683
  pilB Vibrio parahaemolyticus RIMD 2210633

68.149

100

0.681
  pilB Vibrio campbellii strain DS40M4

67.26

100

0.673
  pilB Acinetobacter baumannii D1279779

48.043

100

0.48
  pilB Legionella pneumophila strain ERS1305867

47.069

100

0.472
  pilB Acinetobacter baylyi ADP1

50.098

90.925

0.456
  pilF Neisseria gonorrhoeae MS11

44.071

100

0.443

Multiple sequence alignment