Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   I3Y_RS20910 Genome accession   NZ_CP028379
Coordinates   4027056..4027523 (+) Length   155 a.a.
NCBI ID   WP_000381746.1    Uniprot ID   Q8X892
Organism   Escherichia coli O145 str. RM9872     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4022056..4032523
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I3Y_RS20860 (I3Y_21360) rplP 4022166..4022576 (-) 411 WP_000941212.1 50S ribosomal protein L16 -
  I3Y_RS20865 (I3Y_21365) rpsC 4022589..4023290 (-) 702 WP_000529945.1 30S ribosomal protein S3 -
  I3Y_RS20870 (I3Y_21370) rplV 4023308..4023640 (-) 333 WP_000447529.1 50S ribosomal protein L22 -
  I3Y_RS20875 (I3Y_21375) rpsS 4023655..4023933 (-) 279 WP_001138117.1 30S ribosomal protein S19 -
  I3Y_RS20880 (I3Y_21380) rplB 4023950..4024771 (-) 822 WP_000301864.1 50S ribosomal protein L2 -
  I3Y_RS20885 (I3Y_21385) rplW 4024789..4025091 (-) 303 WP_000617544.1 50S ribosomal protein L23 -
  I3Y_RS20890 (I3Y_21390) rplD 4025088..4025693 (-) 606 WP_000424395.1 50S ribosomal protein L4 -
  I3Y_RS20895 (I3Y_21395) rplC 4025704..4026333 (-) 630 WP_000579833.1 50S ribosomal protein L3 -
  I3Y_RS20900 (I3Y_21400) rpsJ 4026366..4026677 (-) 312 WP_001181004.1 30S ribosomal protein S10 -
  I3Y_RS20910 (I3Y_21410) pilD 4027056..4027523 (+) 468 WP_000381746.1 A24 family peptidase Machinery gene
  I3Y_RS20915 (I3Y_21415) bfr 4027520..4027996 (-) 477 WP_000675515.1 bacterioferritin -
  I3Y_RS20920 (I3Y_21420) bfd 4028069..4028263 (-) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  I3Y_RS20930 (I3Y_21430) tuf 4028446..4029630 (-) 1185 WP_000031783.1 elongation factor Tu -
  I3Y_RS20935 (I3Y_21435) fusA 4029701..4031815 (-) 2115 WP_000124700.1 elongation factor G -
  I3Y_RS20940 (I3Y_21440) rpsG 4031912..4032382 (-) 471 WP_001138043.1 30S ribosomal protein S7 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 17099.29 Da        Isoelectric Point: 8.1343

>NTDB_id=240981 I3Y_RS20910 WP_000381746.1 4027056..4027523(+) (pilD) [Escherichia coli O145 str. RM9872]
MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFYQVCHPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHSWAFLPRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=240981 I3Y_RS20910 WP_000381746.1 4027056..4027523(+) (pilD) [Escherichia coli O145 str. RM9872]
ATGGACACTACCCTTCCCTTTCTAATTTTGTACGCTTGTTTATCAGCCCTACTTTTTTTCTGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACGTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTATCAAGTTTGTCATCCTGATGGTTTAGCCG
ATGCGTTATGGGGGGCAATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGCCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGCGCCTGGCATTCATGGGCTTTCTTACCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTATTGGTCTGCTCATGAGAGGAAAAGAATCATTAAAAAACCCGCTGC
CTTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTATAGGCTGGGATAGTTTGCTGATAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(11-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8X892

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

43.478

89.032

0.387

Multiple sequence alignment